Phylogenetic diversity of Bradyrhizobium strains isolated from root nodules of Lupinus angustifolius grown wild in the North East of Algeria

Mellal, Hanane; Benhizia, Yacine; Boukaous, Leila; Khouni, Sabra; Benguedouar, Ammar; Castellano‐Hinojosa, Antonio; Bedmar, Eulogio J.

doi:10.1016/j.syapm.2019.01.003

Cited by 13 publications

(11 citation statements)

References 35 publications

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“…; Mellal et al . ); with the exception of B. valentinum which nodulates L. mariae‐josephae in alkaline soils in Eastern Spain (Duran et al . ).…”

Section: Discussionmentioning

confidence: 99%

“…The Microvirga LUP strains were isolated form L. cosentinii grown in alkaline soils (pH 7Á5) of the Maamora forest, which is in line with previous results on Microvirga strains recovered from L. micranthus and L. luteus growing in Tunisian alkalinized soils (Msaddak et al 2017a(Msaddak et al , 2017bRejili et al 2019) and suggests that the soil pH is a key factor for determination of the biogeography of the Microvirga species. In contrast to Microvirga, Bradyrhizobium appears to be dominant in acidic to neutral soils (Stepkowski et al 2007;Cardinale et al 2008;Vel azquez et al 2010;S anchez-Cañizares et al 2011;Mellal et al 2019); with the exception of B. valentinum which nodulates L. mariae-josephae in alkaline soils in Eastern Spain (Duran et al 2014).…”

Section: Discussionmentioning

confidence: 99%

“…; Mellal et al . ). Also, fast‐growing strains of the species Ochrobactrum lupini isolated from Lotus honoratus (Trujillo et al .…”

Section: Introductionmentioning

confidence: 97%

“…Lupinus species have been shown to be nodulated by slow-growing rhizobia of the genus Bradyrhizobium, including B. japonicum, B. lupini, B. canariense, B. diazoefficiens and B. elkanii species (Peix et al 2015;Shamseldin et al 2017;Vel azquez et al 2017;Ste z pkowski et al 2018;Mellal et al 2019). Also, fast-growing strains of the species Ochrobactrum lupini isolated from Lotus honoratus (Trujillo et al 2005), of the genus Microvirga from L. texensis (Ardley et al 2012), L. micranthus (Msaddak et al 2017a(Msaddak et al , 2017b and L. subcarnosus (Beligala et al 2017) and Burkholderia have been isolated from the later plant species.…”

Section: Introductionmentioning

confidence: 99%

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Microvirgasp. symbiovar mediterranense nodulatesLupinus cosentiniigrown wild in Morocco

et al. 2019

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Aim To analyse the diversity of nodule‐forming bacteria isolated from Lupinus cosentinii naturally grown in the Maamora cork oak forest (Rabat, Morocco). Methods and Results Of the 31 bacterial strains, four were selected based on their REP‐PCR fingerprinting that were studied by sequencing and phylogenetic analysis of their 16S rRNA, gyrB, dnaK, recA and rpoB housekeeping genes as well as the nodC symbiotic gene. The nearly complete 16S rRNA gene sequence of the four representative strains showed that they are related to Tunisian strains of genus Microvirga isolated from L. micranthus with nucleotide identity values ranging from 98·67 to 97·13%. The single and concatenated sequences of the 16S rRNA, gyrB, dnaK, recA and rpoB housekeeping genes indicated that the L. cosentinii‐isolated strains had 99·2–99·9% similarities with the Tunisian L. micranthus microsymbionts. The nodC gene phylogeny revealed that the Moroccan strains clustered in the newly described mediterranense symbiovar, and nodulation tests showed that they nodulated not only L. cosentinii but also L. angustifolius, L. luteus and L. albus. Conclusions To the best of our knowledge, this is the first report concerning the isolation, molecular identification and phylogenetic diversity of L. cosentinii nodule‐forming endosymbionts and of their description as members of the Microvirga genus. Significance and Impact of the Study In this work, we show that Microvirga sp. can be isolated from root nodules of wild‐grown L. cosentinii in Northeast Africa, that selected strains also nodulate L. angustifolius, L. luteus and L. albus, and that they belong to symbiovar mediterranense. In addition, our data support that the ability of Microvirga to nodulate lupines could be related to the soil pH, its geographical distribution being more widespread than expected.

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“…; Mellal et al . ); with the exception of B. valentinum which nodulates L. mariae‐josephae in alkaline soils in Eastern Spain (Duran et al . ).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…; Mellal et al . ). Also, fast‐growing strains of the species Ochrobactrum lupini isolated from Lotus honoratus (Trujillo et al .…”

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Microvirgasp. symbiovar mediterranense nodulatesLupinus cosentiniigrown wild in Morocco

et al. 2019

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“…Bacteria from a variety of genera, such as Microvirga [6][7][8], Phyllobacterium [8,9] and Bradyrhizobium [6][7][8][9][10][11][12][13][14][15][16] associate symbiotically to lupins. Among these, species from the genus Bradyrhizobium are the most frequently described lupin micro-symbionts.…”

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confidence: 99%

Bradyrhizobium hipponense sp. nov., isolated from Lupinus angustifolius growing in the northern region of Tunisia

Rejili

Off

Brachmann

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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Strain aSej3T was isolated from a root nodule of a Lupinus angustifolius plant growing in Bizerte, Tunisia. 16S rRNA gene analysis placed this strain within the genus Bradyrhizobium . Multilocus sequence analysis (MLSA) including three housekeeping genes (glnII, gyrB and recA) grouped aSej3T together with Bradyrhizobium rifense CTAW71T, Bradyrhizobium cytisi CTAW11T, Bradyrhizobium ganzhouense RITF806T, Bradyrhizobium lupini USDA 3051T and Bradyrhizobium canariense BTA-1T. MLSA with five housekeeping genes (dnaK, glnII, gyrB, recA and rpoB) revealed that this strain shares less than 93.5 % nucleotide identity with other type strains. Genome sequencing and inspection revealed a genome size of 8.83 Mbp with a G+C content of 62.8 mol%. Genome-wide average nucleotide identity and digital DNA–DNA hybridization values were below 87.5 and 36.2 %, respectively, when compared to described Bradyrhizobium species. Strain aSej3T nodulated L. angustifolius plants under axenic conditions and its nodC gene clustered within the genistearum symbiovar. Altogether, the phylogenetic data and the chemotaxonomic characteristics of this strain support that aSej3T represents a new species for which we propose the name Bradyrhizobium hipponense sp. nov. with the type strain aSej3T (=DSM 108913T=LMG 31020T).

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Phylogenetic diversity of indigenous Rhizobium trapped from the natural habitat of Pisum sativum L. in eastern and central Algeria

Gaci

Benhizia

Dems³

et al. 2021

Plant Soil

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Phylogenetic diversity of Bradyrhizobium strains isolated from root nodules of Lupinus angustifolius grown wild in the North East of Algeria

Cited by 13 publications

References 35 publications

Microvirgasp. symbiovar mediterranense nodulatesLupinus cosentiniigrown wild in Morocco

Microvirgasp. symbiovar mediterranense nodulatesLupinus cosentiniigrown wild in Morocco

Bradyrhizobium hipponense sp. nov., isolated from Lupinus angustifolius growing in the northern region of Tunisia

Phylogenetic diversity of indigenous Rhizobium trapped from the natural habitat of Pisum sativum L. in eastern and central Algeria

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