Phylogenetic Framework and Biosurfactant Gene Expression Analysis of Marine <i>Bacillus</i> spp. of Eastern Coastal Plain of Tamil Nadu

Swaathy, Sreethar; Varadharajan, Kavitha; Pravin, A. Sahaya; Sekaran, G.; Mandal, Asit Baran; Gnanamani, A.

doi:10.1155/2014/860491

Cited by 13 publications

(10 citation statements)

References 44 publications

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“…The sfp gene considered as an essential component of peptide synthesis systems and plays a major role in the regulation of surfactinbiosynthesis and gene expression. Srf gene amplification is at 268 bp whereas the expression of the sfp gene amplified at 675 bp [13]. The peptide synthesise for an amino acid moiety of surfactin is encoded by four ORFs in the srfA operon namely SrfAA, SrfAB, SrfAC and SrfAD /SrfA-TE and also contains comS gene lying within the out-of-frame with the srfB [14].…”

Section: 6metabolism Of Biosurfactant Producing Genesmentioning

confidence: 99%

Whole Genome Sequence of Biosurfactant Producing Bacillus Tequilensis

Nayarisseri

Singh

2021

Preprint

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Background: Bioremediation is crucial for recuperate polluted water and soil. By expanding the surface area of substrates, biosurfactants play a vital role in bioremediation. Bioremediation is crucial for recuperate polluted water and soil. By expanding the surface area of substrates, biosurfactants play a vital role in bioremediation. Biosurfactant producing microbes release certain biosurfactant compounds, which are promoted for oil spill remediation. In the present investigation, a biosurfactant producing bacterium Bacillus tequilensis was isolated from Chilika lake, Odisha, India (latitude and longitude: 19.8450 N 85.4788 E). Whole Genome Sequencing (WGS) of Bacillus tequilensis was carried out using Illumina NextSeq 500.Results: The whole genome sequence is 4.47 MB consisting of 4,478,749 base pairs forming a circular chromosome with 528 scaffolds, 4492 protein-encoding genes(ORFs), 81 tRNA genes, and 114 ribosomal RNA transcription units. The total number of raw reads was 4209415, and processed reads were 4058238 with predicted genes of 4492. The whole-genome obtained from the present investigation was used for genome annotation, variant calling, variant annotation and comparative genome analysis with other existing Bacillus species. In this study, a pathway was constructed which describe the biosurfactant metabolism of Bacillus tequilensis. The study identified genes such as SrfAD, SrfAC, SrfAA, SrfAB which are involved in biosurfactant synthesis. Conclusion: The sequence of the genes SrfAD, SrfAC, SrfAA, SrfAB was deposited in Genbank database with accession MUG02427.1, MUG02428.1, MUG02429.1, MUG03515.1 respectively. The whole-genome sequence was submitted to Genbank with an accession RMVO00000000 and the raw reads can be obtained from SRA, NCBI repository using accession: SRX5023292.

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Section: 6metabolism Of Biosurfactant Producing Genesmentioning

confidence: 99%

Whole Genome Sequence of Biosurfactant Producing Bacillus Tequilensis

Nayarisseri

Singh

2021

Preprint

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“…In this respect, Swaathy et al had reported that four bacteria among five isolated cases from marine samples (namely, Bacillus licheniformis [B.licheniformis] ATCC 14580, B. licheniformis DSM13, B. spp. JS chromosome, and B. subtilis BEST 7613) had excellent oil displacement although time had not been stated [27].…”

Section: Drop-collapse Testmentioning

confidence: 99%

A Study on Larvicidal Activity and Phylogenetic Analysis of <i>Staphylococcus epidermidis</i> as a Biosurfactant-Producing Bacterium

Fazaeli¹,

Bahador²,

Hesami³

2021

Pol. J. Environ. Stud.

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Biosurfactants, produced by microorganisms, are surface-active compounds with abundant applications in miscellaneous industries like food, cosmetics, bioremediation, agriculture, and so on. The primary objective of this study was to investigate the larvicidal activity of biosurfactants produced by Staphylococcus epidermidis (S. epidermidis) isolated from petrochemical wastes. As the secondary objective, this study was to perform a phylogenetic analysis of this bacterium. To this end, the type of the extracted biosurfactant was characterized using the thin-layer chromatography (TLC) and the gas chromatography/mass spectrometry (GC-MS). The isolated bacterium was then identified through molecular tests based on 16S ribosomal ribonucleic acid (16S rRNA) and serum response factor (srf ) genes. Likewise, the phylogenetic tree of the bacterium was deduced via the Molecular Evolutionary Genetics Analysis (MEGA-X) software. Besides, the larvicidal activity of the biosurfactant on the larvae of flour beetles, Tribolium castaneum (T. castaneum), was examined. The mean mortality rate and the lethal dose (LD50) were further calculated using the Statistical Analysis System (SAS) and the Probit Analysis-MSChart software. Totally, nine diverse types of colonies were isolated and three of them were found with potential activity to produce biosurfactants. The best bacterium was accordingly selected for subsequent experiments recruiting the oil displacement process and the emulsification index (E24). The extracted biosurfactant was then characterized as a lipopeptide. Moreover, biochemical tests showed that the bacterium was Gram-positive cocci of gamma species. Hemolysis and molecular tests also confirmed that the bacterium was S. epidermidis. As well, the presence of the SRF gene in the isolation was established via the polymerase chain reaction (PCR). The mean mortality rate revealed that the mentioned bacterial biosurfactant at the concentration of 10000 µg/g and on the ninth day of the treatment had the highest larval mortality rate (63.33%). In addition, the LD50 equal to 25001.7 μg/g was calculated for S. epidermidis on the ninth day. The results demonstrated that the biosurfactant

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“…The sfp gene considered as an essential component of peptide synthesis systems and plays a major role in the regulation of surfactinbiosynthesis and gene expression. Srf gene ampli cation is at 268 bp whereas the expression of the sfp gene ampli ed at 675 bp [13]. The peptide synthesise for an amino acid moiety of surfactin is encoded by four ORFs in the srfA operon namely SrfAA, SrfAB, SrfAC and SrfAD /SrfA-TE and also contains comS gene lying within the out-of-frame with the srfB [14].…”

Section: Metabolism Of Biosurfactant Producing Genesmentioning

confidence: 99%

Whole Genome Sequence of biosurfactant producing Bacillus tequilensis

Nayarisseri

2020

Preprint

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We announce the complete genome sequence of Bacillus tequilensis, a biosurfactant producing bacterium isolated from Chilika lake, Odisha, India(latitude and longitude: 19.8450 N 85.4788 E). The genome sequence is 4.47 MB consisting of 4,478,749 base pairs forming a circular chromosome with 528 scaffolds, 4492 protein-encoding genes(ORFs), 81 tRNA genes, and 114 ribosomal RNA transcription units. The total number of raw reads was 4209415 and processed reads were 4058238 with predicted genes of 4492. The whole-genome obtained from the present investigation was used for genome annotation, variant calling, variant annotation and comparative genome analysis with other existing Bacillus species. In this study we constructed a pathway which describe the biosurfactant metabolism of Bacillus tequilensis and identified the genes such as SrfAD, SrfAC, SrfAA which are involved in biosurfactant synthesis. The sequence of the same was deposited in Genbank database with accession MUG02427.1, MUG02428.1, MUG02429.1, MUG03515.1 respectively. The whole-genome sequence was submitted to Genbank with an accession RMVO00000000 and the raw reads can be obtained from SRA, NCBI repository using accession: SRX5023292.

show abstract

Phylogenetic Framework and Biosurfactant Gene Expression Analysis of Marine Bacillus spp. of Eastern Coastal Plain of Tamil Nadu

Cited by 13 publications

References 44 publications

Whole Genome Sequence of Biosurfactant Producing Bacillus Tequilensis

Whole Genome Sequence of Biosurfactant Producing Bacillus Tequilensis

A Study on Larvicidal Activity and Phylogenetic Analysis of <i>Staphylococcus epidermidis</i> as a Biosurfactant-Producing Bacterium

Whole Genome Sequence of biosurfactant producing Bacillus tequilensis

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