Phylogenetic Group of<i> Escherichia coli</i> Isolates from Broilers in Brazilian Poultry Slaughterhouse

Coura, Fernanda Morcatti; Diniz, S.A.; Silva, Marcos X.; Arcebismo, Thiago L. M.; Minharro, Sílvia; Feitosa, Adriana Carla Floresta; Lage, Andrey Pereira; Knöbl, Terezinha; Mussi, Jamili Maria Suhet; Heinemann, Marcos Bryan

doi:10.1155/2017/5898701

Cited by 21 publications

(23 citation statements)

References 33 publications

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“…Phylogenetic typing determines the genetic background or ancestry of an organism as well as differentiating between the pathogenic E. coli strains (B2 and D) from commensals (A and B1) [33,34]. Overall, phylogenetic analysis of APEC strains in this study revealed that majority belonged to Phylogenetic groups A and D. This is in agreement with several studies done elsewhere [20,28,35,36].…”

Section: Discussionsupporting

confidence: 91%

Bacteriophage activity against and characterisation of avian pathogenicEscherichia coliisolated from colibacillosis cases in Uganda

Kazibwe

Katami

Alinaitwe

et al. 2020

Preprint

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A laboratory-based study aimed at establishing a stock of avian pathogenic Escherichia coli (APEC) lytic bacteriophages, for future development of cocktail products for controlling colibacillosis as well as minimizing use of antimicrobial drugs in the poultry production systems in Uganda. Specifically, the study determined the antibiotic susceptibility; phylogenetic categories, occurrence of selected virulence genes among Escherichia coli stock isolates from cases of chicken colibacillosis; and isolation of specific bacteriophages. Fifty six isolates were confirmed as E. coli by standard phenotypic tests. All the 56 (100%) isolates were resistant to at least one antibiotic while 50 (89.3%) isolates were resistant to at least three classes of antimicrobial drugs and were therefore designated as multi-drug resistant. Phylogenetically, APEC isolates mainly belonged to phylogroups A and D which represented 44.6% and 39.3%, respectively. Virulence genes, ompT and iutA were the most frequent with 33 (58.9%) and 32 (57.1%) isolates respectively; while iroN least occurred in 23 (41.1%) isolates. Of the 56 isolates, 69.6% harbored at least one virulence gene, while 50% had at least four virulence genes; hence confirmed as APEC. None of the isolates belonged to the selected serotypes O1, O2 and O78. Seven specific bacteriophages were isolated and their host range, varied from 1.8% to 17.9% (n=56 APEC isolates), while the combined lytic spectrum of all the phages was 25%. Phage stability was negatively affected by increasing temperatures with both UPEC04 and UPEC10 phages becoming undetectable at 70°C; however activity was detected between pH 2 and 12. The high occurrence of APEC isolates with resistance against the commonly used antibiotics supports the need for alternative strategies of bacterial infections control in poultry. The low host range exhibited by the phages calls for search for more candidates before more in-depth studies are done for phage characterization and application.

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Section: Discussionsupporting

confidence: 91%

Bacteriophage activity against and characterisation of avian pathogenicEscherichia coliisolated from colibacillosis cases in Uganda

Kazibwe

Katami

Alinaitwe

et al. 2020

Preprint

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“…Half of investigated isolates likely belong to group D, and are of lesser virulence. Phylogroups A and D were most frequently identified among our MDR E. coli isolates, similar to previous studies (Cortés et al, 2010;Coura et al, 2017).…”

Section: Discussionsupporting

confidence: 90%

Genetic characterization of AmpC and extended-spectrum beta-lactamase (ESBL) phenotypes inEscherichia coliandSalmonellafrom Alberta poultry

Tran

Checkley

Caffrey

et al. 2020

Preprint

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Horizontal gene transfer is an important mechanism which facilitates bacterial populations in overcoming antimicrobial treatment. In this study, a total of 120 Escherichia coli and 62 Salmonella enterica subsp. enterica isolates were isolated from poultry farms in Alberta. Fourteen serovars were identified among Salmonella isolates. Thirty one percent of E. coli isolates were multiclass drug resistant (resistant to ≥ 3 drug classes), while only about 16% of Salmonella isolates were multiclass drug resistant. Among those, eight E. coli isolates had an AmpC-type phenotype, and one Salmonella isolate had an extended-spectrum beta-lactamase (ESBL)-type β-lactamase phenotype. We identified both AmpC-type (blaCMY-2) and ESBL-type (blaTEM) genes in both E. coli and Salmonella isolates. Plasmids from eight of nine E. coli and Salmonella isolates were transferred to recipient strain E. coli J53 through conjugation. Transferable plasmids in above total eight E. coli and Salmonella isolates were also transferred into a lab-made sodium azide-resistant Salmonella recipient through conjugation. The class 1 integrase gene, int1, was detected on plasmids from two E. coli isolates. Further investigation of class 1 integron cassette regions revealed the presence of an aadA gene encoding streptomycin 3''-adenylyltransferase, an aadA1a/aadA2 gene encoding aminoglycoside 3''-O-adenyltransferase, and a putative adenylyltransferase gene. This study provides some insight into potential horizontal gene transfer events of antimicrobial resistance genes between E. coli and Salmonella in poultry production.

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“…Phylogenetic group A isolates were predominate in our study which considered healthy chicken. More often, phylogenetic group A E. coli isolates are commensal organisms, associated with healthy chicken, and rarely cause disease [ 20 , 21 ]. Our findings were in agreement with other workers [ 21 , 22 ].…”

Section: Discussionmentioning

confidence: 99%

“…More often, phylogenetic group A E. coli isolates are commensal organisms, associated with healthy chicken, and rarely cause disease [ 20 , 21 ]. Our findings were in agreement with other workers [ 21 , 22 ]. Conversely, in birds with colibacillosis a majority of E. coli belongs to phylogenetic groups B2 [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic Groups and Antimicrobial Susceptibility Patterns of Escherichia coli from Healthy Chicken in Eastern and Central Uganda

Kabiswa

Nanteza

Majalija

2018

Journal of Veterinary Medicine

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Antimicrobial resistance is an emerging problem in both humans and animals due to misuse and excessive use of drugs. Resistance in commensal E. coli isolates can be used to predict emergence of resistance in other gut microflora. The aim of this study is to determine the phylogenetic groups and antimicrobial resistance patterns of E. coli from healthy chickens in Uganda. The phylogenetic grouping of 120 fecal E. coli isolates from eastern and central Uganda was derived using the triplex PCR assay and their susceptibility patterns determined by agar disc diffusion method to 5 antimicrobial drugs. Most E. coli is segregated into phylogenetic group A comprising 84%, while 12% and 4% were in groups D and B1, respectively. Similarly most E. coli from central (87%) and eastern Uganda (82%) belonged to group A. Overall, 85 (70%) of E. coli were resistant to antimicrobial drugs, of which 72/101 (70%) are in PG A, 10 of 14 (71.4%) in PG D, and 3 of 5 (60%) in PG B1. Significantly, most of the isolates in PG A from both central (66.7%) and (60.6%) eastern Uganda were resistant to one antimicrobial. Resistance to tetracycline alone or in combination with other drugs for central and eastern Uganda in PG A is 51% and 55%, respectively. Multidrug resistance to tetracycline and ciprofloxacin or nalidixic acid was 10% and 18% in isolates from central and 10% and 12% in isolates from eastern region, respectively. Phylogenetic group A accounts for most of the E. coli in chicken from Uganda. No difference in the resistance rates between the phylogenetic groups of E. coli has been observed. The high prevalence of resistant E. coli strains from different phylogenetic groups in healthy chickens suggests antimicrobial drug selection pressure due to excessive drug in the rearing layer chickens.

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Phylogenetic Group of Escherichia coli Isolates from Broilers in Brazilian Poultry Slaughterhouse

Cited by 21 publications

References 33 publications

Bacteriophage activity against and characterisation of avian pathogenicEscherichia coliisolated from colibacillosis cases in Uganda

Bacteriophage activity against and characterisation of avian pathogenicEscherichia coliisolated from colibacillosis cases in Uganda

Genetic characterization of AmpC and extended-spectrum beta-lactamase (ESBL) phenotypes inEscherichia coliandSalmonellafrom Alberta poultry

Phylogenetic Groups and Antimicrobial Susceptibility Patterns of Escherichia coli from Healthy Chicken in Eastern and Central Uganda

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