Phylogenetic Group/Subgroups Distributions, Virulence Factors, and Antimicrobial Susceptibility of Escherichia coli Strains from Urinary Tract Infections in Hatay

Abstract

Introduction: Nosocomial and community acquired urinary tract infections (UTIs) are one of the most encountered infections in the world. Methods: This study aimed to determine the antibiotic susceptibility, phylogeny, and virulence genes of 153 Escherichia coli strains isolated from UTIs. Antimicrobial susceptibility of the isolates to different classes of antimicrobials was determined by the VITEK-2 automated system. Presence of virulence genes and phylogenetic groups were investigated by PCR. Results: Regarding susceptibility to antimicrobials, ampicillin resistance was most abundant (67.3%), followed by amoxicillin-clavulanic acid (50.9%); least abundant was resistance to amikacin (1.3%) and nitrofurantoin (1.3%). Multi drug resistance (MDR) was observed in 34.6% of the isolates, and all isolates were found to be susceptible to imipenem, meropenem and fosfomycine. The majority of the isolates belonged to the phylogenetic group B2 3 (35.9%), followed by A1 (20.9%), D1 (18.9%), D2 (12.4%), A0 (%5.9), B1 (3.9%) and B2 (1.9%). Among E. coli strains examined, 49% had iucD, 32.7% papE-F, 26.1% papC, 15% cnf2, 11.1% sfa, 7.8% cnf1, 1.3% afaE, 1.3% afaD, 1.3% hlyA, 0.7% f17a-A, 0.7% clpG and 0.7% eaeA genes. Conclusions: Our research demonstrated that virulence factors were distributed among different phylogroup/subgroups, which play a role in UTIs pathogenesis in humans. For this reason, complex and detailed studies are required to determine the relationship between virulence factors and specific E. coli strains that cause UTIs in humans. FIGURE 1: PCR amplification of E. coli specific 16S rRNA gene (401 bp).

Multidrug Resistance and Virulence Factors of Escherichia coli Harboring Plasmid-Mediated Colistin Resistance: mcr-1 and mcr-3 Genes in Contracted Pig Farms in Thailand

“…According to phylogenetic grouping, the majority of the isolates in our study were in phylogroups A or B1, predominantly related with commensal strains (27). On the other hand, for the virulent E. coli groups, phylogroup D was not detected in the current study and there was a low frequency of phylogroup B2.…”

Avian pathogenic Escherichia coli (APEC) and uropathogenic Escherichia coli (UPEC): characterization and comparison

“…In the literature, the distribution of APEC strains into phylogenetic groups has varied depending on several factors such as source of isolation and region or country [38][39][40] and has also been related to their in vivo pathogenicity. The distribution of our UPEC strains into phylogenetic groups was similar to those in previous reports [39,[41][42][43].…”

Detection of extended-spectrum beta-lactamase genes among Escherichia coli isolates from urinary tract infection in Mashhad

“…Our results revealed the highest resistance rate for [17,[23][24][25]. However, another study was recorded a lower resistance rate for ampicillin and cotrimoxazole, 67% and 45%, respectively [26]. On the other hand, the highest susceptibility was observed against amikacin, meropenem, nitrofurantoin, and imipenem.…”