Phylogenetic Grouping and Virulence Potential of Extended-Spectrum-β-Lactamase-Producing Escherichia coli Strains in Cattle

Valat, Charlotte; Auvray, Frédéric; Forest, Karine; Métayer, Véronique; Garam, Carine Peytavin de; Madec, Jean‐Yves; Haenni, Marisa

doi:10.1128/aem.00351-12

Cited by 62 publications

(31 citation statements)

References 43 publications

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“…A low prevalence (2/205 isolates, 0.97%) of ESBL-producing E. coli was found only in the cow samples which belonged to the phylogenetic group A and phylogenetic subgroup A1. Our results were in accordance with the results of Valat et al 45 who reported that ESBL-producing E. coli isolates from cattle belonged mainly to the phylogroup A. The low prevalence of ESBLproducing E. coli was similar to the results of Shiraki et al, 46 but it was very lower than that of some previous studies.…”

Section: Discussionsupporting

confidence: 70%

Phylogenetic Grouping and Phenotypic Detection of Extended-Spectrum β-Lactamases Among Escherichia coli From Calves and Dairy Cows in Khuzestan, Iran

Barzan¹,

Gharibi²,

Ghorbanpoor³

et al. 2017

Int J Enteric Pathog

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Background: Food-producing animals are under suspicion for the reservoir and colonization of ESBL (extended-spectrum beta-lactamase)-producing bacteria especially Enterobacteriaceae and therefore infection of the humans with them. The increasing reports on the ESBLs presence in the pathogenic and commensal Escherichia coli isolates have been a concern worldwide. These strains can be attributed to one of the main phylogenetic groups and subgroups. Several studies have shown the relationship between the phylogeny and antimicrobial resistance of E. coli strains. Objectives: The aim of this study was to analyze the phylogenetic group of ESBL-producing E. coli and detect its phenotype using the multiplex polymerase chain reaction (PCR) and combined disk method. Materials and Methods: Two hundred five E. coli fecal isolates were obtained from 103 calves (90 healthy and 13 diarrheic) and 102 dairy cows (healthy) from 8 farms in Khuzestan province, Iran. The triplex PCR method was used to allocate the E. coli isolates based on the presence or absence of 3 genes (chuA, yjaA, and tspE4.C2) to yield 4 definite phylogenetic groups and 7 subgroups. Phenotypic ESBL-producing E. coli was determined using the double disk diffusion method according to the manufacturer's instructions and Clinical & Laboratory Standards Institute (CLSI) guidelines. Results: A total of 65.04% and 22.3% of isolates from calves and 70.5% and 20.5% of isolates from dairy cows belonged to phylogroups B1 and A, respectively. In addition, no isolate from the diarrhoeic calves was found to belong to group B2 and subgroups D2 and A0. A low prevalence (2/205 isolates, 0.97%) of ESBL-producing E. coli was found only in the samples of dairy cows which belonged to the phylogenetic group A and phylogenetic subgroup A1. There was no statistically significant relationship between the phylogenetic group and the production of ESBLs (P = 0.11). There was also no difference between the E. coli isolates from calves and dairy cows in the production of ESBLs (P = 0.5). Conclusion: There was no statistically significant relationship between the phylogenetic group and the production of ESBLs (P = 0.11). There was also no difference between E. coli isolates from calves and dairy cows in the production of ESBLs (P = 0.5). Based on these results, there is a low prevalence of ESBL-producing E. coli in the dairy farms of Khuzestan province. However, further large scale investigations are necessary to control the antibiotic resistance in the human and animal foodstuff.

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Section: Discussionsupporting

confidence: 70%

Phylogenetic Grouping and Phenotypic Detection of Extended-Spectrum β-Lactamases Among Escherichia coli From Calves and Dairy Cows in Khuzestan, Iran

Barzan¹,

Gharibi²,

Ghorbanpoor³

et al. 2017

Int J Enteric Pathog

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“…The association between some phylogroups and antimicrobial resistance patterns is for now poorly understood. Nevertheless, several studies have already highlighted that phylogroup A E. coli are over‐represented within resistant strains isolated in France (Smati et al., 2013), including chromosomal AmpC β‐lactamase overproducers carried by humans (Corvec et al., 2007) as well as ESBL E. coli detected in cattle (Valat et al., 2012) and ampicillin‐resistant isolates from pigs (Bibbal, Dupouy, Prère, Toutain, & Bousquet‐Mélou, 2009). Further studies are still needed to determine if E. coli belonging to phylogroup A are more likely to acquire antimicrobial resistances and why.…”

Section: Discussionmentioning

confidence: 99%

VIM‐1 carbapenemase‐producing Escherichia coli in gulls from southern France

Vittecoq

Laurens

Brazier

et al. 2017

Ecology and Evolution

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Acquired carbapenemases currently pose one of the most worrying public health threats related to antimicrobial resistance. A NDM‐1‐producing Salmonella Corvallis was reported in 2013 in a wild raptor. Further research was needed to understand the role of wild birds in the transmission of bacteria resistant to carbapenems. Our aim was to investigate the presence of carbapenem‐resistant Escherichia coli in gulls from southern France. In 2012, we collected 158 cloacal swabs samples from two gull species: yellow‐legged gulls (Larus michahellis) that live in close contact with humans and slender‐billed gulls (Chroicocephalus genei) that feed at sea. We molecularly compared the carbapenem‐resistant bacteria we isolated through culture on selective media with the carbapenem‐susceptible strains sampled from both gull species and from stool samples of humans hospitalized in the study area. The genes coding for carbapenemases were tested by multiplex PCR. We isolated 22 carbapenem‐resistant E. coli strains from yellow‐legged gulls while none were isolated from slender‐billed gulls. All carbapenem‐resistant isolates were positive for bla VIM ‐1 gene. VIM‐1‐producing E. coli were closely related to carbapenem‐susceptible strains isolated from the two gull species but also to human strains. Our results are alarming enough to make it urgently necessary to determine the contamination source of the bacteria we identified. More generally, our work highlights the need to develop more bridges between studies focusing on wildlife and humans in order to improve our knowledge of resistant bacteria transmission routes.

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“…Most of the E. coli isolates had untypeable O and H antigens (OUT, HUT). Neither E. coli clone ST131 (O25:H4) nor enterohemorrhagic E. coli O157, O26, or O111 or other serotypes commonly isolated from human infections (8) were detected from the 13 isolates.…”

Section: Fig 2 Pfge Patterns and Cluster Analysis Of 13 Ctx-m-producimentioning

confidence: 99%

“…ince 2000, Escherichia coli and other Enterobacteriaceae species producing CTX-M-type extended-spectrum ␤-lactamases (ESBLs) (CTX-M) have been commonly isolated from community-acquired extraintestinal infections in humans and their companion animals (1, 2, 3, 4), from food-producing animals (3,5,6,7,8), and from retail meats, including chicken, beef, and pork (3), worldwide. The CTX-M-type genes are assumed to have been transferred separately to plasmids, including complex class 1 integrons and transposons (9), from chromosomes of different Kluyvera species (i.e., Kluyvera ascorbata, K. georgiana, and K. cryocrescens) that live in water, soil, and human and animal intestinal tracts; therefore, CTX-M has been divided into five clusters (CTX-M-1, CTX-M-2, CTX-M-8, CTX-M-9, and CTX-M-25) from base sequence homology (1, 9).…”

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Genetic Characteristics of CTX-M-Type Extended-Spectrum-β-Lactamase (ESBL)-Producing Enterobacteriaceae Involved in Mastitis Cases on Japanese Dairy Farms, 2007 to 2011

et al. 2013

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h Sixty-five CTX-M-2/15/14 extended-spectrum-␤-lactamase-producing Enterobacteriaceae were isolated from 258,888 mastitic milk samples from Japanese dairy farms between 2007 and 2011. CTX-M-2-producing Klebsiella pneumoniae and CTX-M-15-producing Escherichia coli were the predominant strains isolated. There was no predominant clonal type, and clonal diversity was found even in strains isolated from a single farm. Since 2000, Escherichia coli and other Enterobacteriaceae species producing CTX-M-type extended-spectrum ␤-lactamases (ESBLs) (CTX-M) have been commonly isolated from community-acquired extraintestinal infections in humans and their companion animals (1, 2, 3, 4), from food-producing animals (3,5,6,7,8), and from retail meats, including chicken, beef, and pork (3), worldwide. The CTX-M-type genes are assumed to have been transferred separately to plasmids, including complex class 1 integrons and transposons (9), from chromosomes of different Kluyvera species (i.e., Kluyvera ascorbata, K. georgiana, and K. cryocrescens) that live in water, soil, and human and animal intestinal tracts; therefore, CTX-M has been divided into five clusters (CTX-M-1, CTX-M-2, CTX-M-8, CTX-M-9, and CTX-M-25) from base sequence homology (1, 9). CTX-M confers resistance against penicillins, oxyimino-cephalosporins, and monobactams (1, 4). Recently, the CTX-M-15-producing E. coli ST131 (O25: H4) clone has emerged as a multidrug-resistant pandemic strain affecting humans worldwide (4).Bovine mastitis is the most common disease affecting dairy cattle (10). Both E. coli and Klebsiella pneumoniae often cause life-threatening clinical mastitis (5, 11). The incidence of bovine mastitis has been reported to be higher in Japan (30 to 35 cases per 100 cow-years at risk) (12) than in North America, Europe, and New Zealand (10 to 30 cases per 100 cow-years) (10). Only a few classes of antimicrobials are approved for the treatment of mastitis in Japan; however, large amounts of antimicrobials are used for mastitis treatment, creating selective pressure for drug-resistant organisms (13). In our previous report, we showed that Japanese dairy cattle might be a source of CTX-M-15/2/14-and CMY-2-producing Enterobacteriaceae (7). However, few studies have reported the prevalence of Enterobacteriaceae producing CTX-M in bovine mastitis (5). The aims of this study were to determine the genetic characteristics, antimicrobial susceptibility, and genetic relatedness of ESBL-and plasmid-mediated AmpC ␤-lactamaseproducing Enterobacteriaceae isolated from bovine mastitis cases.Screening of ESBLs. Bacterial cultures were carried out using standard procedures on a total of milk samples from 258,888 quarters obtained from 176,808 cows affected by (mainly clinical) mastitis on 1,000 dairy farms in the Nemuro Subprefecture of Hokkaido Prefecture, Japan, between February 2007 and April 2011 (14). Streptococcus spp. (mainly Streptococcus uberis) and Enterococcus spp., coagulase-negative staphylococci, Staphylococcus aureus, E. coli, and Klebsiella spp. were the...

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Phylogenetic Grouping and Virulence Potential of Extended-Spectrum-β-Lactamase-Producing Escherichia coli Strains in Cattle

Cited by 62 publications

References 43 publications

Phylogenetic Grouping and Phenotypic Detection of Extended-Spectrum β-Lactamases Among Escherichia coli From Calves and Dairy Cows in Khuzestan, Iran

Phylogenetic Grouping and Phenotypic Detection of Extended-Spectrum β-Lactamases Among Escherichia coli From Calves and Dairy Cows in Khuzestan, Iran

VIM‐1 carbapenemase‐producing Escherichia coli in gulls from southern France

Genetic Characteristics of CTX-M-Type Extended-Spectrum-β-Lactamase (ESBL)-Producing Enterobacteriaceae Involved in Mastitis Cases on Japanese Dairy Farms, 2007 to 2011

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