Phylogenetic groups and cephalosporin resistance genes of Escherichia coli from diseased food-producing animals in Japan

Asai, Tetsuo; Masani, Kei; Sato, Chizuru; Hiki, Mototaka; Usui, Masaru; Baba, Kohtaro; Ozawa, Manao; Harada, Kazuki; Aoki, Hiroshi; Sawada, Takuo

doi:10.1186/1751-0147-53-52

Cited by 65 publications

(53 citation statements)

References 23 publications

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“…These results were almost similar to other studies which demonstrated that the isolates of phylogroup A were prevalent in the intestinal tracts of most animals 24,37 and phylogroup B1 was dominant among isolates from healthy cattle. 38 In accordance with the previous reports in Iran, 23,39 this study showed that the phylogroup B1 was dominant in calves. The phylogroup B2 had also the least frequency, both in calves and dairy cows, and these outcomes confirmed the previous investigations.…”

Section: Discussionsupporting

confidence: 80%

Phylogenetic Grouping and Phenotypic Detection of Extended-Spectrum β-Lactamases Among Escherichia coli From Calves and Dairy Cows in Khuzestan, Iran

Barzan¹,

Gharibi²,

Ghorbanpoor³

et al. 2017

Int J Enteric Pathog

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Background: Food-producing animals are under suspicion for the reservoir and colonization of ESBL (extended-spectrum beta-lactamase)-producing bacteria especially Enterobacteriaceae and therefore infection of the humans with them. The increasing reports on the ESBLs presence in the pathogenic and commensal Escherichia coli isolates have been a concern worldwide. These strains can be attributed to one of the main phylogenetic groups and subgroups. Several studies have shown the relationship between the phylogeny and antimicrobial resistance of E. coli strains. Objectives: The aim of this study was to analyze the phylogenetic group of ESBL-producing E. coli and detect its phenotype using the multiplex polymerase chain reaction (PCR) and combined disk method. Materials and Methods: Two hundred five E. coli fecal isolates were obtained from 103 calves (90 healthy and 13 diarrheic) and 102 dairy cows (healthy) from 8 farms in Khuzestan province, Iran. The triplex PCR method was used to allocate the E. coli isolates based on the presence or absence of 3 genes (chuA, yjaA, and tspE4.C2) to yield 4 definite phylogenetic groups and 7 subgroups. Phenotypic ESBL-producing E. coli was determined using the double disk diffusion method according to the manufacturer's instructions and Clinical & Laboratory Standards Institute (CLSI) guidelines. Results: A total of 65.04% and 22.3% of isolates from calves and 70.5% and 20.5% of isolates from dairy cows belonged to phylogroups B1 and A, respectively. In addition, no isolate from the diarrhoeic calves was found to belong to group B2 and subgroups D2 and A0. A low prevalence (2/205 isolates, 0.97%) of ESBL-producing E. coli was found only in the samples of dairy cows which belonged to the phylogenetic group A and phylogenetic subgroup A1. There was no statistically significant relationship between the phylogenetic group and the production of ESBLs (P = 0.11). There was also no difference between the E. coli isolates from calves and dairy cows in the production of ESBLs (P = 0.5). Conclusion: There was no statistically significant relationship between the phylogenetic group and the production of ESBLs (P = 0.11). There was also no difference between E. coli isolates from calves and dairy cows in the production of ESBLs (P = 0.5). Based on these results, there is a low prevalence of ESBL-producing E. coli in the dairy farms of Khuzestan province. However, further large scale investigations are necessary to control the antibiotic resistance in the human and animal foodstuff.

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Section: Discussionsupporting

confidence: 80%

Phylogenetic Grouping and Phenotypic Detection of Extended-Spectrum β-Lactamases Among Escherichia coli From Calves and Dairy Cows in Khuzestan, Iran

Barzan¹,

Gharibi²,

Ghorbanpoor³

et al. 2017

Int J Enteric Pathog

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“…A few articles are available on detection of ESBL-E. coli from healthy or diseased swine population in Asian countries such as China (Duan et al, 2006;Hu et al, 2013), South Korea (Tamang et al, 2013) and Japan (Asai et al, 2011). In India, mostly exotic breeds of pigs are reared by organized farming and indigenous or non-descript breeds are reared by backyard farming and garbage belts (Banik et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Approaches to characterize extended spectrum beta-lactamase/beta-lactamase producing Escherichia coli in healthy organized vis-a-vis backyard farmed pigs in India

Samanta

Joardar

Mahanti

et al. 2015

Infection, Genetics and Evolution

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“…Animal bacteria do not persist in human intestinal microflora mainly due to lack of host specificity [38]. In this context, ESBLproducing E. coli isolated from animals can be different from clinical isolates [39]. Moreover, mathematical models of antimicrobial usage in the farm industry and the emergence of drug resistance in humans strongly suggests that infection control and prudent medical antimicrobial usage rather than elimination of the use of antimicrobials in animals will more likely reduce the prevalence of antimicrobial resistance in hospitals.…”

Section: Veterinary Antimicrobial Therapies and Esbl Emergence In Hummentioning

confidence: 99%

An overview of extended-spectrum beta-lactamases in veterinary medicine and their public health consequences

Nobrega

Brocchi

2014

J Infect Dev Ctries

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Serious human and animal infections caused by bacteria are usually treated with beta-lactams. Extended-spectrum beta-lactamases (ESBLs) constitute the most clinically and economically important enzymes that are able to hydrolyze and inactivate beta-lactam antibiotics in veterinary medicine. The spread of ESBLs represents a serious threat to healthcare systems, drastically undermining therapeutic options. The relationship between drug usage and the emergence of resistance has been extensively reported. Nevertheless, the use of antimicrobials in veterinary medicine and the emergence of ESBLs in animals remains a matter of debate. Moreover, there is still controversy about whether antibiotic usage in farm animals poses a potential public health risk. This review will (i) deal with aspects related to the presence of ESBLs in veterinary medicine, (ii) its link with human medicine, and (iii) discuss strategies to be implemented to preserve antimicrobial effectiveness. New insights relative to old questions concerning antimicrobial use in domestic animals are also presented.

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Phylogenetic groups and cephalosporin resistance genes of Escherichia coli from diseased food-producing animals in Japan

Cited by 65 publications

References 23 publications

Phylogenetic Grouping and Phenotypic Detection of Extended-Spectrum β-Lactamases Among Escherichia coli From Calves and Dairy Cows in Khuzestan, Iran

Phylogenetic Grouping and Phenotypic Detection of Extended-Spectrum β-Lactamases Among Escherichia coli From Calves and Dairy Cows in Khuzestan, Iran

Approaches to characterize extended spectrum beta-lactamase/beta-lactamase producing Escherichia coli in healthy organized vis-a-vis backyard farmed pigs in India

An overview of extended-spectrum beta-lactamases in veterinary medicine and their public health consequences

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