Phylogenetic lineage of GII.17 norovirus identified among children in South-South, Nigeria

Abstract

Objectives Norovirus is a major cause of diarrhea among children worldwide. This present report highlight’s the genetic homology patterns of GII.17 noroviruses detected among children under-5 years of age with diarrhea in the South-South, region of Nigeria. Stool specimens were collected from 300 children with diarrhea and analyzed for norovirus using conventional reverse transcriptase-Polymerase Chain Reaction. Sequencing of the capsid region was performed to genotype the strains Results 36/300 (12.0%) of patients were positive for norovirus by RT-PCR. 7/36 (19.4%) (5 GI.3 and 2 GI.5) were GI others where typed to be GII. All GII.17 norovirus identified in this study, 3/29 (10.3%) where typed to belong to the recently discovered GII.17 Kawasaki strain. This study report for the first time the detection of norovirus GII.17 Kawasaki strain in South-South, region of Nigeria.

Molecular Detection of Human Norovirus among Children (0-5 Years) Attending Selected Hospitals in Kano Metropolis, Kano State, Nigeria

“…This indicates that NoV genogroup II are the most circulating among the study participants, and possibly in Kano state and the country at large. This finding is consistent with other results from Nigeria including that of Osazuwa et al 32 in South-south and Ayolabi et al 28 from Lagos state, and also from other countries like Indonesia 35 and The Democratic Republic of Congo 37 . Interestingly, our result is in agreement with the global findings, indicating genogroup II as the most prevalent genogroup in humans.…”

Human rotavirus VP4 and VP7 genetic diversity and detection of GII norovirus in Ibadan as Nigeria introduces rotavirus vaccine

“… 23 However, norovirus GII.17 is rapidly emerging as the most dominant strain in some parts of the world. 24 Osazuwa and Grobler 25 reported the presence of norovirus GII.17 Kawasaki among Nigerian children younger than 5 with diarrhea. Although the molecular epidemiology of these viruses in developed countries has been extensively described, the exact role of noroviruses in acute gastroenteritis in children in sub-Saharan Africa is unclear.…”

Development of a Recombinase-Aided Amplification Assay for Rapid Detection of Human Norovirus GII.4

“…Established methods for detecting norovirus, such as conventional RT-PCR (Osazuwa and Grobler et al, 2020), droplet digital PCR (Persson and Eriksson et al, 2018), TaqMan-based real-time RT-PCR (Gao and Wang et al, 2019), recombinase polymerase ampli cation (RPA) assays (Moore and Jaykus, 2017), and loop-mediated isothermal ampli cation (LAMP) assays (Yaren and Bradley et al, 2016), have multiple limitations.…”