Phylogenetic Patterns of Human Respiratory Picornavirus Species, Including the Newly Identified Group C Rhinoviruses, during a 1-Year Surveillance of a Hospitalized Patient Population in Italy

Piralla, Antonio; Baldanti, Fausto; Gerna, Giuseppe

doi:10.1128/jcm.01814-10

Cited by 50 publications

(55 citation statements)

References 23 publications

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“…Previous reports suggested that HRV-C is associated with more severe illness than HRV-A or HRV-B (19,32,43,47). More recent studies show that HRV-A as well as HRV-C are the most prevalent HRVs identified in hospitalized children (7,27,37,41,49), while HRV-C infection is associated with an increase in hospitalization events in atopic children (4). The role of HRVs in respiratory disease is currently an area of intense research, and the emerging data indicate a significant role for HRV-C in lower respiratory tract disease, particularly in children (10,21,22,26,30,38,48).…”

mentioning

confidence: 99%

Infection and Propagation of Human Rhinovirus C in Human Airway Epithelial Cells

Hao¹,

Bernard²,

Patel³

et al. 2012

J Virol

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Human rhinovirus species C (HRV-C) was recently discovered using molecular diagnostic techniques and is associated with lower respiratory tract disease, particularly in children. HRV-C cannot be propagated in immortalized cell lines, and currently sinus organ culture is the only system described that is permissive to HRV-C infection ex vivo. However, the utility of organ culture for studying HRV-C biology is limited. Here, we report that a previously described HRV-C derived from an infectious cDNA, HRV-C15, infects and propagates in fully differentiated human airway epithelial cells but not in undifferentiated cells. We demonstrate that this differentiated epithelial cell culture system supports infection and replication of a second virus generated from a cDNA clone, HRV-C11. We show that HRV-C15 virions preferentially bind fully differentiated airway epithelial cells, suggesting that the block to replication in undifferentiated cells is at the step of viral entry. Consistent with previous reports, HRV-C15 utilizes a cellular receptor other than ICAM-1 or LDLR for infection of differentiated epithelial cells. Furthermore, we demonstrate that HRV-C15 replication can be inhibited by an HRV 3C protease inhibitor (rupintrivir) but not an HRV capsid inhibitor previously under clinical development (pleconaril). The HRV-C cell culture system described here provides a powerful tool for studying the biology of HRV-C and the discovery and development of HRV-C inhibitors.

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mentioning

confidence: 99%

Infection and Propagation of Human Rhinovirus C in Human Airway Epithelial Cells

Hao¹,

Bernard²,

Patel³

et al. 2012

J Virol

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“…These children, however, had no neurological symptoms, unlike those in reports from Colorado and California (20,21). Additionally, EV-D68 infection in Thailand paralleled peak influenza outbreak during the rainy season, which differs from the EV-D68 pattern observed in the summer and autumn elsewhere (26,27).…”

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confidence: 46%

Prevalence and Phylogenetic Characterization of Enterovirus D68 in Pediatric Patients with Acute Respiratory Tract Infection in Thailand

et al. 2016

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SUMMARY: Enterovirus D68 (EV-D68) is associated with severe lower respiratory tract infection and neurological abnormalities including acute myelitis and cranial nerve dysfunction. To determine whether an increased incidence of EV-D68 occurs in Southeast Asia, we retrospectively tested specimens collected from Thai pediatric patients who were less than 5 years of age and presented with acute respiratory tract infections between 2012 and 2014. Reverse transcription-polymerase chain reaction and nucleotide sequencing of the 5 ? -UTR/VP2 region were used to identify EV-D68. We also examined the epidemiological pattern of EV-D68 since 2009, when it was first identified in Thailand, and compiled records of clinical manifestations in children with confirmed EV-D68 infection. From 837 samples, 5 samples (0.6z) tested positive for EV-D68. All patients presented with viral pneumonia and required hospitalization. Phylogenetic analysis of the VP4/VP2 regions revealed that EV-D68 strains circulating in Thailand between 2012 and 2014 were closely related to strains reported in Japan, United Kingdom, China, and France. Continued surveillance of probable EV-D68-associated severe respiratory tract infection and the development of a rapid diagnostic test for EV-D68 are essential in supporting awareness and facilitating disease prevention and control.Enterovirus D68 (EV-D68) is a subtype of Enterovirus enterovirus D within the family Picornaviridae. It was first isolated in the United States in 1962 from respiratory specimens obtained from 4 pediatric patients with pneumonia and bronchiolitis (1). Since then, EV-D68 infections have occurred in small numbers and accounted for only 26 reports among enteroviruses detected in the United States during the 36-year surveillance period from 1970-2005 (2). Between 2008-2010, clusters of EV-D68 emerged in the Philippines, Japan, the Netherlands, and the United States (3). EV-D68 infections have

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“…After the first isolation in 1962 from four children with respiratory illness, EV68 has been isolated rarely. 3,4,[7][8][9][10][11][12] Detailed clinical and epidemiological data are mostly limited or even lacking.…”

Section: Discussionmentioning

confidence: 99%

Upsurge of human enterovirus 68 infections in patients with severe respiratory tract infections

Rahamat‐Langendoen

Riezebos‐Brilman

Borger

et al. 2011

Journal of Clinical Virology

105

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Phylogenetic Patterns of Human Respiratory Picornavirus Species, Including the Newly Identified Group C Rhinoviruses, during a 1-Year Surveillance of a Hospitalized Patient Population in Italy

Cited by 50 publications

References 23 publications

Infection and Propagation of Human Rhinovirus C in Human Airway Epithelial Cells

Infection and Propagation of Human Rhinovirus C in Human Airway Epithelial Cells

Prevalence and Phylogenetic Characterization of Enterovirus D68 in Pediatric Patients with Acute Respiratory Tract Infection in Thailand

Upsurge of human enterovirus 68 infections in patients with severe respiratory tract infections

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