Phylogenetic position and virulence apparatus of the pear flower necrosis pathogen Erwinia piriflorinigrans CFBP 5888T as assessed by comparative genomics

Smits, Theo H. M.; Rezzonico, Fabio; López, Marı́a M.; Blom, Jochen; Goesmann, Alexander; Frey, Jürg E.; Duffy, Brion

doi:10.1016/j.syapm.2013.04.003

Cited by 18 publications

(12 citation statements)

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“…It was observed that there is genetic variation of the T6SS gene clusters detected for E. amylovora strains infecting Spiraeoideae compared to E. amylovora strains infecting Rubus [ 64 ], which supports the hypothesis that the T6SSs could represent a potential role in host-specificity. Additionally, the closely related pear pathogens Erwinia pyrifoliae DSM 12163 T and Erwinia pyriflorinigrans CFBP 5888 T and the epiphyte Erwinia tasmaniensis Et1/99 possess only the two homologous T6SS gene clusters 1 and 2, while the third cluster is absent from their genome sequences [ 35 , 65 , 66 ].…”

Section: Discussionmentioning

confidence: 99%

Role of the type VI secretion systems during disease interactions of Erwinia amylovora with its plant host

et al. 2017

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BackgroundType VI secretion systems (T6SS) are widespread among Gram-negative bacteria and have a potential role as essential virulence factors or to maintain symbiotic interactions. Three T6SS gene clusters were identified in the genome of E. amylovora CFBP 1430, of which T6SS-1 and T6SS-3 represent complete T6SS machineries, while T6SS-2 is reduced in its gene content.ResultsTo assess the contribution of T6SSs to virulence and potential transcriptomic changes of E. amylovora CFBP 1430, single and double mutants in two structural genes were generated for T6SS-1 and T6SS-3. Plant assays showed that mutants in T6SS-3 were slightly more virulent in apple shoots while inducing less disease symptoms on apple flowers, indicating that T6SSs have only a minor effect on virulence of E. amylovora CFBP 1430. The mutations led under in vitro conditions to the differential expression of type III secretion systems, iron acquisition, chemotaxis, flagellar, and fimbrial genes. Comparison of the in planta and in vitro transcriptome data sets revealed a common differential expression of three processes and a set of chemotaxis and motility genes. Additional experiments proved that T6SS mutants are impaired in their motility.ConclusionThese results suggest that the deletion of T6SSs alters metabolic and motility processes. Nevertheless, the difference in lesion development in apple shoots and flower necrosis of T6SS mutants was indicative that T6SSs influences the disease progression and the establishment of the pathogen on host plants.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-4010-1) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Role of the type VI secretion systems during disease interactions of Erwinia amylovora with its plant host

et al. 2017

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“…Furthermore, the overall position of Pantoea within the Enterobacteriaceae has not been conclusively established. The genus Tatumella is commonly regarded as its sister taxon (Brady et al, 2008, Brady et al, 2010a, Brady et al, 2012, although other intergeneric relationships have also been reported (Brady et al, 2008, Brady et al, 2010b, Brady et al, 2012, Kamber et al, 2012, Smits et al, 2013, Glaeser and Kämpfer, 2015, Gueule et al, 2015. We hypothesize that these inconsistent inter-and intrageneric relationships are mainly due to the small datasets often being used for phylogenetic inference (Brady et al, 2008, Brady et al, 2010b, Glaeser and Kämpfer, 2015.…”

Section: Introductionmentioning

confidence: 89%

Phylogenomic resolution of the bacterial genus Pantoea and its relationship with Erwinia and Tatumella

Palmer

Steenkamp

Coetzee

et al. 2017

Antonie van Leeuwenhoek

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Investigation of the evolutionary relationships between related bacterial species and genera with a variety of lifestyles have gained popularity in recent years. For analysing the evolution of specific traits, however, a robust phylogeny is essential. In this study we examined the evolutionary relationships among the closely related genera Erwinia, Tatumella and Pantoea, and also attempted to resolve the species relationships within Pantoea. To accomplish this, we used the whole genome sequence data for 35 different strains belonging to these three genera, as well as nine outgroup taxa. Multigene datasets consisting of the 1039 genes shared by these 44 strains were then generated and subjected to maximum likelihood phylogenetic analyses, after which the results were compared to those using conventional multi-locus sequence analysis (MLSA) and ribosomal MLSA (rMLSA) approaches. The robustness of the respective phylogenies was then explored by considering the factors typically responsible for destabilizing phylogenetic trees. We found that the nucleotide datasets employed in the MLSA, rMLSA and 1039-gene datasets contained significant levels of homoplasy, substitution saturation and differential codon usage, all of which likely gave rise to the observed lineage specific rate heterogeneity. The effects of these factors were much less pronounced in the amino acid dataset for the 1039 genes, which allowed reconstruction of a fully supported and resolved phylogeny. The robustness of this amino acid tree was also supported by different subsets of the 1039 genes. In contrast to the smaller datasets (MLSA and rMLSA), the 1039 amino acid tree was also not as sensitive to long-branch attraction. The robust and well-supported evolutionary hypothesis for the three genera, which confidently resolved their various inter- and intrageneric relationships, represents a valuable resource for future studies. It will form the basis for studies aiming to understand the forces driving the divergence and maintenance of lineages, species and biological traits in this important group of bacteria.

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“…Unlike Erwinia amylovora, the causal agent of fire blight, E. piriflorinigrans attacks only blossoms and no other parts of plants are apparently affected (Roselló et al 2006). Other usual fire blight hosts in the same area did not display any symptoms of infection with this Erwinia species (Smits et al 2013).…”

Section: Discussionmentioning

confidence: 86%

“…The phytopathogen E. amylovora enters hosts through natural openings such as nectarthodes and wounds. The disease develops as blossom, shoot, trunk, or root stock blight depending of the plant tissue affected by the organism (Smits et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of Erwinia piriflorinigrans causal agent flower necrosis of red poppy

Amirabad

Khodakaramian

2017

Australasian Plant Pathol.

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In 2016, necrosis symptoms was observed on stem and flower of red poppy (Papaver rhoeas) in Hamadan province of Iran. Symptomatic samples were collected and suspicious bacterial agent was isolated on nutrient agar medium. The phenotypic features of the bacterial strains were characterized and some molecular traits were examined. The bacterial strains phenotypically showed a high similarity to the members of Enterobacteriaceae, in particular Erwinia piriflorinigrans. All tested strains were pathogenic on red poppy and blossoms of pear under greenhouse condition. The phylogenetic analysis based on 16S rRNA and atpD genes sequences showed that representative strains were very similar to Erwinia piriflorinigrans. This is the first report of the presence of Erwinia piriflorinigrans as a causal agent of flower necrosis of red poppy worldwide.

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Phylogenetic position and virulence apparatus of the pear flower necrosis pathogen Erwinia piriflorinigrans CFBP 5888T as assessed by comparative genomics

Cited by 18 publications

References 61 publications

Role of the type VI secretion systems during disease interactions of Erwinia amylovora with its plant host

Role of the type VI secretion systems during disease interactions of Erwinia amylovora with its plant host

Phylogenomic resolution of the bacterial genus Pantoea and its relationship with Erwinia and Tatumella

Isolation and characterization of Erwinia piriflorinigrans causal agent flower necrosis of red poppy

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