PHYLOGENETIC POSITION OF BOTRYOCOCCUS BRAUNII (CHLOROPHYCEAE) BASED ON SMALL SUBUNIT RIBOSOMAL RNA SEQUENCE DATA¹

Abstract

A small subunit ribosomal RNA (16S‐like rRNA) in the hydrocarbon‐rich microalga Botryococcus braunii Kützing (Chlorophyceae) was amplified using RNA polymerase chain reaction, and its sequence was determined. The sequence data of B. braunii were analyzed with those of several other algae in order to determine phylogenetic relationships among these algae. Phylogenetic analysis indicated B. braunii to be a member of the Chlorophyta and possibly related to Characium vacuolatum and Dunaliella parva.

Liquid Fuel Production Using Microalgae

“…Analyses of small subunit ribosomal RNA (rRNA) sequence data have facilitated clarification of phylogenetic relationships among microalgae 15), 16) . The phylogenetic position of B. braunii has been determined using 16S-like rRNA sequence data 17) . The 16S-like rRNA in the hydrocarbon-rich microalga B. braunii was amplified using RNA polymerase chain reaction, and its sequence was determined.…”

Efect of Gamma 60Co Irradiation on The Growth, Lipid Content and Fatty Acid Composition of Botryococcus sp. Microalgae

“…is one of microalgae type included in the Chlorophyceae class with the highest lipid content compared to other types of microalgae, which amounted to 75% of the dry biomass weight (Sawayama et al, 1995;Chisti, 2007). Botryococcus has a main fatty acid profile such as oleic acid (C18: 1, 54.9%), palmitic acid (C16: 0, 12.2%), linolenic acid (C18: 3, 5.5%), stearic acid ( C18: 0, 3.9%) and linoleic acid (C18: 2, 5.5%), these fatty acids can be used for biodiesel feedstock ( Knothe, 2008).…”

Phylogenetic Position of the Phacotaceae Within the Chlamydophyceae as Revealed by Analysis of 18S rDNA and rbcL Sequences

“…Analysis based on morphological and/or molecular data contributed to the idea that a set of coccalorganized chlorophytes also belong to the Chlamydophyceae, i.e., Characium vacuolatum , and Botryococcus braunii (Sawayama et al 1995). Lewis et al (1992) compared morphological and molecular data and showed that chlorococcalean species that were characterized by a clockwise orientation of the basal bodies were also phylogenetically closely related to the Chlamydophyceae.…”