Phylogenetic position of Vicia montbretii and Lens species in relation to Vicia subgenus Cracca (Fabaceae): morphological and isozyme evidence

Leht, Malle; Jaaska, Vello

doi:10.1002/fedr.201800019

Cited by 3 publications

(1 citation statement)

References 25 publications

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“…Some other taxa from close genera such as Vicia or Lathyrus have been assigned to Lens, for instance Vicia montbretii has been classified as L. montbretii, but there is a general agreement that they do not belong to Lens (Cubero et al 2009;Ladizinsky and Abbo 2015;Smykal et al 2015;Leht and Jaaska 2019).…”

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confidence: 99%

Tackling Lentil Biotic Stresses in the Genomic Era

Vega

Garcı́a

Gutiérrez-González

et al. 2022

Genomic Designing for Biotic Stress Resistant Pulse Crops

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Lentils are already one of the main pulses of the world, as they are one of the main sources of protein for humans. As a crop, they are also gaining momentum because the rusticity and tolerance to water scarcity of some varieties are a good fit with the current global warming trend and climate change in general. However, while the harvested area and overall production have drastically increased over the last decades, yield has only experienced very modest increments. The reasons are two-fold. First, pathogens are affecting the crop as never before, likely due to not only the changing climate but also to the expansion of lentil cultivation to new geographic areas. Second, genomics-aided breeding is far behind many other crops. This is in partly due to the lack of genomic tools currently available to researchers. Progress is being made to adopt high-throughput genomic methods, and researchers will be able to tackle lentil gene discovery and breeding for pathogen resistance and other biotic stresses more efficiently in the coming years. We outline the current situation, novel findings, and prospects of lentil research for biotic stresses. IntroductionLentil (Lens culinaris Medik. subsp. culinaris) is one of the first domesticated species in the Fertile Crescent and along with barley, emmer wheat and einkorn wheat, pea, chickpea, and flax was part of the set of crops that defined the beginnings of the Neolithic transition to agriculture in this part of the World. The origin of the cultivated form is the wild L. culinaris subsp. orientalis (Boiss.) Ponert (syn. L. orientalis Boiss.). A recent publication by Liber et al. (2021) suggests that phylogenetics, population structure, and archeological data coincide in a lentil domestication prolonged in time in Southwest Asia, with two different domesticated gene pools. From the

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confidence: 99%

Tackling Lentil Biotic Stresses in the Genomic Era

Vega

Garcı́a

Gutiérrez-González

et al. 2022

Genomic Designing for Biotic Stress Resistant Pulse Crops

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Interspecific variation of seed morphological and micro‐morphological traits in the genus Vicia (Fabaceae)

Han

Raveendar

Lee

et al. 2020

Microscopy Res & Technique

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Seed macro‐ and micro‐morphology were analyzed to evaluate their capacity to discriminate species in the genus Vicia (Fabaceae). To assess the interspecific variation of the taxa in the genus Vicia, 41 accessions were obtained from the USDA‐ARS germplasm collection in the USA and 19 accessions were collected from Korea. Seed morphological characteristics such as shape, color, mottling, finish, length, width, diameter, hilum shape, hilum color, hilum length, and lens distance from the hilum were examined under a stereomicroscope. Testa texture characteristics such as testa pattern, papillae type, density, height, ribbing, surface deposits, and peaks topped with wax were examined under scanning electron microscopy. Various gross‐morphological traits of seeds of Vicia species have been analyzed and compared. The present study revealed significant variation in testa traits. Testa were papillose and papillose with mounds, the latter being observed only in Vicia lathyroides. The present study revealed 20 key traits that could be used to diagnose Vicia species and classify them.

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Proteolytic degradation of seed proteins of vetch species (Vicia L. subgenus Vicia) of section Peregrinae Kupicha during SDS-electrophoresis and its prevention

Konarev,

Eggi,

Aleksandrova

2024

Biotehnologiâ i selekciâ rastenij

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Background. Due to its simplicity and good reproducibility, SDS-electrophoresis of seed proteins is widely used for investigating the gene pool of legumes and other plants, for species and varietal identification, analyzing the intraspecific variability, and registering collection material. The data obtained by this method agree well with the DNA analysis results complement them. Typically, legume seed proteins, including representatives of the genus Vicia L., show clear informative SDS electrophoretic profiles. When analyzing seed accessions of vetch species of the section Peregrinae Kupicha using standard approaches previously developed at VIR and approved by ISTA (the International Seed Testing Association), clear electrophoretic protein profiles could not be obtained for many accessions. This called into question the applicability of standard approaches to identifying vetch species in the section Peregrinae. The objective of the work was to clarify the nature of seed proteins degradation in representatives of the Peregrinae section and to find ways to prevent it to ensure the possibility of carrying out species identification and registration of all accessions in the vetch collection using a unified approach. Material and methods. Seed proteins of a number of vetch species Vicia L. from sections Bithynicae (B. Fedtsch.) Maxted, Hypechusa (Alef.) Aschers. et Graebner, Microcarinae Maxted and Peregrinae, members of the subgenus Vicia, were analyzed by SDS-electrophoresis using the standard method of protein extraction from flour with 0.025 M Tris-glycine buffer pH 8.3 at room temperature and its modifications, including heating the extract at 80°C or 100°C with or without the addition of 2-mercaptoethanol, as well as the addition of cysteine and serine protease inhibitors. Results and discussion. An analysis of seed proteins of representatives of most sections of the subgenus Vicia yielded informative species-specific protein profiles, whereas species of the section Peregrinae were characterized by the protein profiles, which indicated protein degradation, and species of this section differed in the frequency of such profile occurrence. While such profiles were obtained for all seeds of seven accessions of V. aintabensis Boiss & Hausskn. ex Boiss differing in geographical origin, year and place of regeneration, and 12 out of 13 of V. peregrine L. accessions demonstrated profiles of partially or completely degraded proteins, complete seed protein profiles were obtained for six out of nine V. michauxii Sprengel accessions. A change in conditions for protein isolation, namely replacement of their extraction from flour with Tris-glycine buffer pH 8.3 at room temperature with extraction in the same buffer by a short-term heating at 100°C in the presence of 2-mercaptoethanol, made it possible to obtain complete protein profiles for all accessions representing the section Peregrinae. The protein profiles of representatives of other vetch sections, as well as the profile of soybean proteins used as a standard for legume species identification, did not differ from the original ones under the modified conditions. Conclusions. The obtained results suggest that protein degradation in species of the Peregrinae section is associated with the abnormal activity of endogenous seed proteases under standard protein extraction conditions, and this trait is determined genotypically. A new modification of the method for isolating proteins from seeds makes it possible to apply the generally accepted approaches based on SDS-electrophoresis in the analysis of the gene pool of the Peregrinae section of the subgenus Vicia, as well as other vetch species.

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Phylogenetic position of Vicia montbretii and Lens species in relation to Vicia subgenus Cracca (Fabaceae): morphological and isozyme evidence

Cited by 3 publications

References 25 publications

Tackling Lentil Biotic Stresses in the Genomic Era

Tackling Lentil Biotic Stresses in the Genomic Era

Interspecific variation of seed morphological and micro‐morphological traits in the genus Vicia (Fabaceae)

Proteolytic degradation of seed proteins of vetch species (Vicia L. subgenus Vicia) of section Peregrinae Kupicha during SDS-electrophoresis and its prevention

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