Phylogenetic positions and taxonomic assignments of the systematically controversial genera,Spirotrachelostyla,UroleptopsisandTunicothrix(Protozoa, Ciliophora, Stichotrichia) based on small subunit rRNA gene sequences

Huang, Jie; Yi, Zhenzhen; Al‐Farraj, Saleh A.; Song, Weibo

doi:10.1080/14772000.2010.508502

Cited by 22 publications

(26 citation statements)

References 34 publications

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“…2009a,b; Paiva & Silva‐Neto 2009, Paiva et al. 2009; Huang et al. 2010) have shown that analyses combining morphological and morphogenetic characters with molecular characters are more likely to yield reliable results, especially in some taxonomically confused groups.…”

Section: Introductionmentioning

confidence: 99%

Phylogeny and systematic revision of the family Pseudokeronopsidae (Protista, Ciliophora, Hypotricha), with description of a new estuarine species of Pseudokeronopsis

Chen

Clamp²,

Song

2011

Zoologica Scripta

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Chen, X., Clamp, J. C. & Song, W. (2011). Phylogeny and systematic revision of the family Pseudokeronopsidae (Protista, Ciliophora, Hypotricha), with description of a new estuarine species of Pseudokeronopsis. —Zoologica Scripta, 40, 659–671. The family Pseudokeronopsidae is a taxon of hypotrich ciliates with a history of uncertainty regarding its systematic and phylogenetic relationships to other members of the order Urostylida. Phylogenetic analyses of pseudokeronopsids were made using all available molecular and morphological information, and the patterns of morphogenesis of the group were reinvestigated. Results clearly demonstrated that the genera Thigmokeronopsis and Apokeronopsis are not confamilial with Pseudokeronopsis and Nothoholosticha but, instead, must be transferred to the family Urostylidae. A new estuarine species, Pseudokeronopsis erythrina sp. n. was discovered and described using a combination of morphological and molecular characters. Species of Pseudokeronopsis are difficult to characterize but can be distinguished from one another by a combination of morphological and morphogenetic characters. Pseudokeronopsis similis is a freshwater species that has significant morphogenetic differences with other members of the genus and may not be congeneric with them.

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Section: Introductionmentioning

confidence: 99%

Phylogeny and systematic revision of the family Pseudokeronopsidae (Protista, Ciliophora, Hypotricha), with description of a new estuarine species of Pseudokeronopsis

Chen

Clamp²,

Song

2011

Zoologica Scripta

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“…Their phylogenetic placement results in polyphyly of traditional urostylids in such analyses (e.g. Schmidt et al, 2007;Paiva et al, 2009;Huang et al, 2010;Li et al, 2010a (Berger, 2006), but nevertheless showing the retroextendians to be polyphyletic. This is basically due to the position of Apokeronopsis spp.+Thigmokeronopsis stoecki Shao et al, 2008, away from Pseudokeronopsis spp., a phylogenetic pattern that is consistent with previous studies (e.g.…”

Section: Comparison With Related Speciesmentioning

confidence: 99%

Morphology and 18S rDNA phylogeny of Hemicycliostyla sphagni (Ciliophora, Hypotricha) from Brazil with redefinition of the genus Hemicycliostyla

Paiva

Borges

Silva‐Neto

et al. 2012

International Journal of Systematic and Evolutionary Microbiology

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Morphology of the urostylid ciliate Hemicycliostyla sphagni Stokes, 1886, the type of Hemicycliostyla Stokes, 1886, is investigated based on live and protargol-impregnated specimens from a Brazilian population. The absence of transverse cirri, which has been considered the main diagnostic feature of Hemicycliostyla, separating it from Pseudourostyla Borror, 1972, was found to vary within the studied population, with 50 % of the specimens exhibiting inconspicuous and/or rudimentary transverse cirri. A redefinition of Hemicycliostyla was possible based on combined features of interphase and divisional morphogenesis: Retroextendia Berger, 2006, with bi- or multicoronal frontal cirral pattern; fronto-terminal cirri present; multiple left and right marginal cirral rows that replicate independently via within-row development, each parental row producing one primordium per divider; caudal cirri lacking; and presence/absence of transverse cirri may be intrapopulationally variable. Phylogenetic analyses of the 18S rDNA marker unambiguously placed H. sphagni as sister group of Pseudourostyla franzi Foissner, 1987, which is herein transferred to Hemicycliostyla as Hemicycliostyla franzi comb. nov.

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“…To minimize sequence errors, the Ex Taq (TaKaRa, Japan) which shows 4-fold higher fidelity compared with the conventional Taq, with an error rate of about 2.2 × 10 −6 , was used for PCR amplification. The purification of PCR products, cloning and sequencing were performed according to previous reports (Huang et al 2010;Zhang et al 2010). Multiple positive bacterial clones were sequenced for each species.…”

Section: Dna Extraction Amplification and Sequencingmentioning

confidence: 99%

Variations and evolution of polyubiquitin genes from ciliates

Liu

Shi

Gong

2013

European Journal of Protistology

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Polyubiquitin genes from seven ciliate species were amplified, cloned and sequenced. It is estimated that Strombidium sulcatum, Euplotes vannus, E. rariseta and Anteholosticha manca have a polyubiquitin gene of 3 repeats, and A. parawarreni, Paramecium caudatum and Pseudokeronopsis flava 4 repeats. The newly obtained ubiquitins mostly differ from that of humans by 1-5 residues in amino acid sequences. A neighbor-joining tree constructed based on monomeric ubiquitin genes supports the monophyly of an assemblage comprising the litostomateans and some oligohymenophoreans, but not the class Spirotrichea. The monomers from the same species are generally placed together and highly supported for the class Litostomatea, the genera Paramecium and Ichthyophthirius, but not for other species. The non-synonymous/synonymous rate ratio (dN/dS) at the protein level are less than 1, and the synonymous nucleotide differences per synonymous site (p S ) from intraspecific comparisons are fairly high (0.02-0.72). These results indicate that ciliates have not only the conserved, but also some quite divergent, polyubiquitin genes and confirm that the polyubiquitin genes in ciliates evolve according to the birth-and-death mode of evolution under strong purifying selection.

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Phylogenetic positions and taxonomic assignments of the systematically controversial genera,Spirotrachelostyla,UroleptopsisandTunicothrix(Protozoa, Ciliophora, Stichotrichia) based on small subunit rRNA gene sequences

Cited by 22 publications

References 34 publications

Phylogeny and systematic revision of the family Pseudokeronopsidae (Protista, Ciliophora, Hypotricha), with description of a new estuarine species of Pseudokeronopsis

Phylogeny and systematic revision of the family Pseudokeronopsidae (Protista, Ciliophora, Hypotricha), with description of a new estuarine species of Pseudokeronopsis

Morphology and 18S rDNA phylogeny of Hemicycliostyla sphagni (Ciliophora, Hypotricha) from Brazil with redefinition of the genus Hemicycliostyla

Variations and evolution of polyubiquitin genes from ciliates

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