Phylogenetic relatedness of methicillin-resistant Staphylococcus aureus isolates from the host community and Syrian refugees in Duhok Governorate based on 16S rRNA

Rasheed, Narin A.; Abdulrahman, Rezheen F.; Hussein, Nawfal R

doi:10.1016/j.ijregi.2022.06.003

Cited by 3 publications

(2 citation statements)

References 31 publications

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“…According to a study, MRSA strains found in Syrian refugees in Duhok Governorate, KRG-Iraq, were subjected to amplification using 16S rRNA and were observed to exhibit genetic relatedness in sequence, indicating a shared ancestry from which they have evolved. The proximity of neighboring countries may have contributed to the observed similarity in MRSA strains between the two research groups, as it may have facilitated the transmission of MRSA strains across borders [24].…”

Section: Discussionmentioning

confidence: 99%

Genotyping of Methicillin Resistant Staphylococcus aureus Clinical Isolates in Sulaimani City using Pulsed -Field Gel Electrophoresis

Naji,

Arif

2024

Tikrit J. Pure Sci.

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Methicillin-resistant Staphylococcus aureus (MRSA) strains cause serious nosocomial infections. The objective of this study was to detect and find the epidemiological relatedness among MRSA isolates which was collected in Emergency burn-and-plastic surgery hospital in Sulaimani city using Pulsed-filed gel electrophoresis (PFGE). Routine methods were used for detection of Staphylococcus aureus, further molecular confirmation was done by PCR assays targeting 16S rRNA gene. The cefoxitin-disc diffusion assay was used for detection MRSA, which is subsequently confirmed by the presence of the mecA gene. PFGE was chosen to observe the genetic relatedness of 10 MRSA strains using SmaI restriction endonuclease. All the isolates (100%, 50/50) carried 16S rRNA gene specific for S.aureus and (42%, 21/50) exhibited resistance to cefoxitin and harboured the mecA gene, thereby being classified as MRSA. Among 10 MRSA isolates, the PFGE pattern were grouped into four clusters. Three isolates belonging to cluster (C3) were indistinguishable, 4 isolates belonging to cluster (C4) were closely related, 2 isolates of the second cluster (C2) were possibly related and the first cluster (C1) with 1 MRSA isolate was unrelated. This finding shows 30% of the samples had indistinguishable fingerprints with no changes in banding profiles, while 40% of the samples had closely related patterns. PFGE is a good discriminatory tool for typing MRSA strains.

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Section: Discussionmentioning

confidence: 99%

Genotyping of Methicillin Resistant Staphylococcus aureus Clinical Isolates in Sulaimani City using Pulsed -Field Gel Electrophoresis

Naji,

Arif

2024

Tikrit J. Pure Sci.

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“…Antibiotic sensitivity patterns have been studied in Kurdistan region of Iraq [21][22][23][24][25][26]. These studies showed that the antibiotic resistance rates are high [27][28][29]. Hence, this study is conducted to determine prevalence of Gram-positive uropathogens among UTI patients, and to assess their antimicrobial susceptibility pattern to commonly used antimicrobial agent to help with doctors to determine appropriate empirical treatment as well as to detect any possible trend of change in sensitivity during a 12 years period from 2010 to 2022 in Duhok Province, Kurdistan Region, Iraq…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Resistance Rates in Gram-positive Uropathogens in Duhok city, Kurdistan Region of Iraq

Mohamed

2023

Preprint

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BackgroundUrinary tract infections (UTIs) are among the most common infections world-wide. Antibiotic resistance is an important medical problem because there is an increasing trend of antibiotic resistance worldwide making it harder to eliminate uropathogens. Antibiotic resistance shows a geographical variation. Hence, local studies are necessary to determine prevalence of uroptahogens among UTI patients. Thus, this study was conducted to determine prevalence of uropathogens among UTI patients and their antimicrobial susceptibility pattern from the data of 12 years period from 2010 to 2022 in Duhok Province, Kurdistan Region, IraqMaterials and methodsThis study was conducted by retrieving 12-year laboratory records between 2010–2022. data were collected from Azadi teaching hospital in Duhok city in Kurdistan region in northern Iraq. uropathogen species were identified by routine laboratory methods. Antimicrobial sensitivity testing was performed manually and by Vitek-2 automated susceptibility system.ResultsThe results of gram-positive urine pathogens of 249 patients showed Staphylococcus haemolyticus (20.9%) was the most common isolated pathogen, followed by staphylococcus aureus (7.6%), Streptococcus Agalactiae (6.4%) and Enterococcus spp. (6%). S. haemolyticus had highest resistance to nitrofurantoin (61.5%) and lowest amikacin (34.3%). S. aureus showed highest resistance to penicillin 73.3% and highest sensitivity to amikacin (78.9%).in our study, Streptococcus agalactiae had the highest sensitivity to vancomycin (%87.5). 93.3% of enterococcus spp. were resistant to tetracycline.ConclusionUTIs are more common in female than in male and coagulase-negative Staph species (Staphylococcus spp) are the most commonly isolated pathogens. This study found antimicrobial resistance to commonly prescribed antibiotics are high. Hence, an urgent plan to control antimicrobial resistance is necessary in our area.

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Molecular distribution of biocide resistance genes and susceptibility to biocides among vancomycin resistant Staphylococcus aureus (VRSA) isolates from intensive care unit (ICU) of cardiac hospital- A first report from Pakistan

Asghar,

Ain,

Zaidi

et al. 2023

Heliyon

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Phylogenetic relatedness of methicillin-resistant Staphylococcus aureus isolates from the host community and Syrian refugees in Duhok Governorate based on 16S rRNA

Cited by 3 publications

References 31 publications

Genotyping of Methicillin Resistant Staphylococcus aureus Clinical Isolates in Sulaimani City using Pulsed -Field Gel Electrophoresis

Genotyping of Methicillin Resistant Staphylococcus aureus Clinical Isolates in Sulaimani City using Pulsed -Field Gel Electrophoresis

Antimicrobial Resistance Rates in Gram-positive Uropathogens in Duhok city, Kurdistan Region of Iraq

Molecular distribution of biocide resistance genes and susceptibility to biocides among vancomycin resistant Staphylococcus aureus (VRSA) isolates from intensive care unit (ICU) of cardiac hospital- A first report from Pakistan

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