Phylogenetic relationship of non-typeable Haemophilus influenzae isolated in Malaysia

Abstract

The epidemiology of non-typeable Haemophilus influenzae (NTHi) remains poorly understood. We therefore sought to determine the genetic relationship of 25 NTHi isolated from various states in Malaysia using multilocus sequence typing (MLST). The majority of isolates were obtained from sputum. There were 24 novel sequence types (STs). Eight isolates were single-locus variants, the remainder being singletons. Clustering was not based on clinical site of isolation or geographical origin. Despite the limited number of isolates examined in this study, we demonstrate that NTHi isolates in Malaysia are diverse and warrant further investigation. A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT

Vaccinomics-driven proteome-wide screening of Haemophilus influenzae for the prediction of common putative vaccine candidates

“…Among the infections caused by this bacterium, community acquired pneumonia (CAP), conjunctivitis, epiglottitis, septicaemia, otitis media, meningitis, bronchitis and sinusitis are of major clinical importance Ulanova et al 2014) and particularly common in adults and children (Wroblewski, et al, 2013). Its infection has also been associated with cystic fibrosis and chronic obstructive pulmonary disease (Pinto et al 2018;Mohd-Zain et al 2015). Globally, respiratory diseases associated with H. influenzae cause an estimated 1.9 million deaths per year (Kumavath et al 2014).…”