Phylogenetic relationships among Southern Balkan Rutilus species inferred from cytochrome b sequence analysis: Micro-geographic resolution and taxonomic implications

Phylogeny, phylogeography and hybridization of Caucasian barbels of the genusBarbus(Actinopterygii, Cyprinidae)

“…However, the divergence between Caucasian and West Balkan cave shrimp Troglocaris was estimated to occur earlier, at approximately 6-11 Mya based on COI and 16S markers (Zakšek et al, 2007). Earlier diverged lineages inside the Ponto- The present study confirmed the relatedness of two Balkanian barbel species that inhabited the Aegean Sea drainage to Caucasian barbels (Levin et al, 2012) Tsoumani et al, 2014).…”

Molecular data suggest a multispecies complex of Phoxinus (Cyprinidae) in the Western Balkan Peninsula

“…1). This considerably more structured phylogeographic 200 pattern than previously proposed may explain why taxonomists 201 have been unable to clearly distinguish putative P. lumarieul from 202 P. phoxinus (Bianco, 2014 in morphological (Talevski et al, 2009;Bogutskaya et al, 2012) and 216 molecular studies (Milosević et al, 2011;Tsoumani et al, 2014). 217 Moreover, we found three additional clades along the Adriatic river …”

“…According to the present study, P.lumaireul could coincide with Clade 1a (northern Adriatic: samples 271 from River Ticino, Po drainage), but the cyt b genetic distance from 272 neighbouring Clades 1b and 1c is only 2%. Several authors quote 273 cyt b differences of between 2% and 11% when trying to delimitate 274 species or evolutionary significant units(Gilles et al, 2010; Schönhuth et al, 2012;Tsoumani et al, 2014). Nevertheless, they 276 agree that a number of genetic markers (mitochondrial and 277 nuclear), as well as morphological and geographical characterisation, should be combined for final species or linage determination.Whereas the largest distances observed in the present study were 280 from 7% to 11% for Clades 6, 7 and 8, and the three clades are also281 supported by the network based on the nuclear RAG1 gene and 282 partially by their geographical distribution, taken together such 283 findings might be sufficient evidence for determination of a new 284 species.…”

Evaluating the genetic status of the IUCN vulnerable endemic Macedonian shad (Alosa macedonica, Vinciguerra, 1921) from Lake Volvi

“…Notably, a similar colonization route (i.e. from the Ponto-Caspian region) was also proposed for the Rutilus population inhabiting Lake Volvi (Tsoumani et al, 2014). The combination of both RFLP and sequence data sets yielded 11 composite genotypes (haplotypes), whose haplotypic relationships are shown in Fig.…”

“…Notably, a similar colonization route (i.e. from the Ponto‐Caspian region) was also proposed for the Rutilus population inhabiting Lake Volvi (Tsoumani et al., ).…”