Phylogenetic relationships among Spanish Barbus species (Pisces, Cyprinidae) shown by RAPD markers

Abstract

Random amplified polymorphic DNA (RAPD) markers were used to estimate the population structure and phylogenetic relationships among the eight species of the genus Barbus that inhabit the Iberian Peninsula. Ten random oligodecamers were used to amplify DNA from 232 fish sampled from 15 populations. A total of 270 markers were detected that revealed low levels of genetic variability. The conclusions of cluster analysis indicate two main branches and three welldifferentiated groups: north-eastern, Mediterranean and

Genetic structure of Spanish white‐clawed crayfish (Austropotamobius pallipes) populations as determined by RAPD analysis: reasons for optimism

“…However, ancient historical events such as population fragmentation, recolonizations from refugia during the ice ages Gouin et al, 2003;Trontelj et al, 2005; Die´guez-Urebiondo et al, in press), or the formation of fluvial basins, may also have influenced their present structure. Such influences have been seen to operate on many animal and plant species (Taberlet et al, 1998;Callejas and Ochando, 2002).…”

Comparative Assessment of Genetic Variability in the Populations of Endemic and Endangered Yellow Catfish, Horabagrus brachysoma (Teleostei: Horabagridae), Based on Allozyme, RAPD, and Microsatellite Markers

“…Allozymes (Markert and Moller 1959), random amplified polymorphic DNA (Williams et al 1990;Welsh and McClelland 1990), and microsatellites (Tautz 1989) are the common genetic markers used for population genetics and pedigree analysis and for taxonomic discrimination of species (Klinbunga et al 2000;Piel and Nutt 2000;Callejas and Ochando 2002;Ma and Chen 2011;Sen et al 2010;Singh et al 2010;Chaturvedi et al 2011). Several authors have reported comparative assessments of these three markers in fishes (Cagigas et al 1999;Sun et al 1999;Mandal et al 2009).…”

“…Liu et al (1998), however, reported a slightly higher percentage of polymorphic RAPD loci (61.05%) in the channel catfish Ictalurus punctatus and I. furcatus. The higher percentage of polymorphism scored with RAPD markers in the present study is probably due to preferential amplification of noncoding repetitive regions of the genome that may elude natural selection (Callejas and Ochando 2002).…”

Molecular identification of five Indian sciaenids (pisces: perciformes, sciaenidae) using RAPD markers

“…The RAPD markers have been successfully used in genetic diversity studies in the past for several species (Williums et al, 1990;Callejas & Ochando, 2002;Govindaraju & Jayasankar, 2004;Das et al, 2005). Our study generated RAPD profiles with species specific bands in five sciaenid species through eight random primers for the first time.…”

“…Each individual was scored for the presence or absence of a particular amplified band. The RAPD profiles for the five species were classified as described by Callejas & Ochando (2002). The RAPD genotype data were analysed using software TFPGA (Ver.…”