Phylogenetic relationships of five Oriental Apanteles species-groups (Hymenoptera: Braconidae: Microgastrinae) by concatenating four molecular markers

Aman-Zuki, Ameyra; Mohammed, Muhamad Azmi; Zain, Badrul Munir Md.; Yaakop, Salmah

doi:10.1016/j.aspen.2019.01.012

Cited by 3 publications

(3 citation statements)

References 85 publications

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“…Similarly, molecular characters including COI, 16S rDNA, and 28S rDNA were used to study the evolutionary transitions in parasitic wasps belonging to Apocrita in recent times 47 . Aman-zuki and coworkers through their work also suggested the usefulness of molecular markers in resolving the phylogeny of species belonging to Microgastrine wasps 48 .…”

Section: Molecular Characterisationmentioning

confidence: 99%

Phylogenetic Analysis of the Economically Important Hymenopterans using cytochrome oxidase 1 Enzyme Sequences

Charul

Bandral

Shivalika

et al. 2023

Biosci., Biotech. Res. Asia

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Hymenoptera is the fourth diverse and the most economically important insect order comprising of bees, wasps, ants, sawflies etc. Being an important part of ecosystem, their conservation is of utmost importance. The first step towards conservation strategies is the identification of the species. The traditional morphological approach can sometimes lead to misidentification due to a lack of expertise. DNA barcoding using the small genomic fragments has been identified as an efficient tool in the identification as well as the phylogenetic analysis of the species. In the present study, we used the COI gene sequences as a tool for the characterization of Hymenoptera from different parts of the Jammu region. The collected samples were proceeded for the isolation of DNA, PCR for amplification of the COI gene, and then sequenced by Sanger dideoxy method. A total of 22 COI sequences belonging to 18 different species were successfully generated. Among which eight species sequences (Tachytes sp., Bombus trifasciatus, Rhynchium carnaticum, Ropalidia brevita, Lasioglossum marginatum, Camponotus pennsylvanicus, Tapinoma melanocephalum, Formica rufibarbis) are the novel contribution in the global database. NJ tree using the K2P model with 1000 bootstrap supporting values has been used to study the phylogeny of the species. Sequence analysis shows high AT content (67-77%) in the COI region of Hymenopterans. The generated COI sequence analysis also revealed less than 1% intra-specific divergence in the examined taxa, while the interspecific distances ranged between 8% to 38%. This study added significantly to the databases of DNA barcodes of Hymenopterans species from Jammu region.

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Section: Molecular Characterisationmentioning

confidence: 99%

Phylogenetic Analysis of the Economically Important Hymenopterans using cytochrome oxidase 1 Enzyme Sequences

Charul

Bandral

Shivalika

et al. 2023

Biosci., Biotech. Res. Asia

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“…A total of 25 ml PCR mixture consisted of 0.5 ml of 0.2mM dNTPs, 10 pmol of each primer, 1.25U of Taq polymerase, and 1 ml of 15mM MgCl 2 from Vivantis were prepared. PCR was performed using MyGene MG96G Thermalcycler or Thermocycler Perkin Elmer 240 under different conditions for each primer combination, starting with initial denaturation for 3 minutes at 94°C, followed by 39 cycles; denaturation for 1 min at 92°C, annealing for 1 min at 47°C, an extension for 1 min at 72°C and final extension for 5 mins at 72°C (Mohammed, Aman-Zuki, Yusof, S., Md-Zain, & Yaakop 2017;Halim et al, 2018;Aman-Zuki, Mohammed, Md Zain, & Yaakop 2019). Two sets of universal primers used were COI [Ron (5' GGA TCA CCT CAT ATA GCA TTC CC 3') (Forward); Nancy (5' CCC GGT AAA AAT TAA AAT ATA AAC TTC 3') (Reverse)] (Simon et al, 1994;Monteiro & Pierce, 2001) and Cytb [(CB-J-10933 5' TCT TTT TGA GGA GCW ACW GTW ATT AC 3'; CB-N-11367 5' AAT TGA ACG TAA AAT WGT RTA AGC AA 3'] (Smith, Kambhampati, Völkl, & Mackauer, 1999; to yield 600 bp and 560 bp fragments.…”

Section: Dna Extraction and Pcr Amplificationmentioning

confidence: 99%

Proof on the Divergence Times of Two Sympatric Species, Rhynchophorus ferrugineus and R. vulneratus (Coleoptera: Curculionidae) by Molecular Clock Analysis

Aman-Zuki

Ghazali

Badrulisham

et al. 2021

jers

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“…This is likely because a combination of three markers in this study has resulted in a total of 27 informative characters, compared to 3, 8 and 16 in COI, Cytb and 28S, respectively. In order to obtain more support values for the nodes on the phylogenetic trees (Wortley et al, 2005), combination markers are suggested and this was supported by Aman-Zuki et al (2019) who had proven that by combining mitochondrial and nuclear datasets, more robust phylogeny of the parasitic wasp, the Apanteles group was observed. In addition, Meemongkolkiat et al (2019) and Baird et al (2017) also presented more resolved phylogeography and phylogeny in both species of study by implementing nuclear and mtDNA.…”

Section: P R E S Smentioning

confidence: 99%

NEW INSIGHTS INTO THE PHYLOGEOGRAPHY OF THE OIL PALM PEST, Metisa plana TOWARDS ITS MANAGEMENT CONTROL

Badrulisham¹

2021

JOPR

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Metisa plana Walker, has contributed as an important pest in the palm oil industry. Even though various studies have been conducted on M. plana there is still insufficient information on the relationships among the populations to illustrate the distribution of this species. We aim to investigate the phylogeography of the M. plana populations by combining data of cytochrome c oxidase subunit I (COI), cytochrome b (Cytb), and 28S markers. The M. plana specimens have been sampled from 10 heavily infested oil palm plantations in Peninsular Malaysia. A total of 145 sequences of three markers were combined and implemented for the phylogenetic analyses, Neighbour-Joining (NJ) and Bayesian Inference (BI). Both phylogenetic trees showed mixing of individuals of the M. plana inter-populations, despite a very distinct geographical isolation.Based on findings from the haplotype analyses; haplotype diversity (Hd= 0.96089), haplotype number ( 27), haplotype network, and haplotype tree; all supported the genetic exchange, indicating the possibility of gene flow. The genetic exchange occurs probably due to the flying ability of the male moth or caused by the human activities between the various plantations that accidently resulted in the transportation and movement of the pest larvae. Interestingly, the haplotype network has also been visualised in estimating the origin of the infestation, which most probably originated from three different plantations, resulting in the rapid outbreaks of the M. plana infestation. These fundamental data are very crucial and informative in the effort to strategise the management control of the M. plana.

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Phylogenetic relationships of five Oriental Apanteles species-groups (Hymenoptera: Braconidae: Microgastrinae) by concatenating four molecular markers

Cited by 3 publications

References 85 publications

Phylogenetic Analysis of the Economically Important Hymenopterans using cytochrome oxidase 1 Enzyme Sequences

Phylogenetic Analysis of the Economically Important Hymenopterans using cytochrome oxidase 1 Enzyme Sequences

Proof on the Divergence Times of Two Sympatric Species, Rhynchophorus ferrugineus and R. vulneratus (Coleoptera: Curculionidae) by Molecular Clock Analysis

NEW INSIGHTS INTO THE PHYLOGEOGRAPHY OF THE OIL PALM PEST, Metisa plana TOWARDS ITS MANAGEMENT CONTROL

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