Phylogenetically Diverse Aerobic Anoxygenic Phototrophic Bacteria Isolated from Epilithic Biofilms in Tama River, Japan

Hirose, Shouichi; Matsuura, Kiyotaka; Haruta, Shin

doi:10.1264/jsme2.me15209

Cited by 27 publications

(25 citation statements)

References 55 publications

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“…Some of the previous studies reported the presence of the particle-attached AAnPB. Hirose et al [24] detected the AAnPB species in the epilithic biofilm of Tama River. Most of first cultures of AAnPB species were isolated from the surface of seaweed [25].…”

Section: Determination Of Absolute Cell Abundances Of the Total Bactementioning

confidence: 99%

Abundance and Spatial Distribution of Aerobic Anoxygenic Phototrophic Bacteria in Tama River, Japan

Sato‐Takabe

Hirose

Hori

et al. 2020

Water

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Aerobic anoxygenic phototrophic bacteria (AAnPB) are widely distributed and regarded as key players driving the carbon cycle in surface water of global oceans, coastal and estuary areas and in other freshwater environments (e.g., ponds and lakes). However, the abundance and spatial distribution of AAnPB in rivers is much less well-known. Here we investigated the variation of the absolute cell abundances of the total bacteria, AAnPB and cyanobacteria, at four different sites in Tama River, Japan, and the spatial distribution (i.e., free-living or particle-attached existence form) of AAnPB at two out of the four sites using infra-red epifluorescence microscopy. Free-living cell abundances for the total bacteria, AAnPB and cyanobacteria were 1.6–3 × 105, 1.5–4.4 × 104 and <3.2 × 104 cells mL−1, respectively. The free-living AAnPB accounted for 6.1%–19.6% of the total bacterial abundance in the river. The peaks of the AAnPB and cyanobacteria abundances were found at the same site, suggesting that the AAnPB could potentially coexist with cyanobacteria. Meanwhile, the particle-attached AAnPB were observed at the two sites of the river, accounting for 52.2% of the total bacteria abundance in the particle. Our results showed the existence and aggregation form of AAnPB in the riverine environment.

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Section: Determination Of Absolute Cell Abundances Of the Total Bactementioning

confidence: 99%

Abundance and Spatial Distribution of Aerobic Anoxygenic Phototrophic Bacteria in Tama River, Japan

Sato‐Takabe

Hirose

Hori

et al. 2020

Water

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“…Due to their pivotal roles in the biogeochemical cycle ( 19 , 35 ), the study of lotic ecology has been attracting increasing interest ( 24 , 42 , 54 ). In attempts to model ecological mechanisms in lotic ecosystems, bacteria are essentially viewed as primary contributors ( 41 , 47 ). Thus, the dynamics of a bacterial community along a river have received increasing attention.…”

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confidence: 99%

Co-occurrence Network Reveals the Higher Fragmentation of the Bacterial Community in Kaidu River Than Its Tributaries in Northwestern China

Bai

Cai

et al. 2018

Microbes and environments

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Rivers and their tributaries sculpt the earth’s surface, and play an important role in substance circulation and energy flow. Bacteria are involved in most biogeochemical processes in the fluvial ecosystem; however, their pattern distribution in a river and its tributaries has not yet been investigated in detail. In the present study, high-throughput sequencing was employed to examine bacterial communities and their co-occurrence networks between Kaidu River and its nine tributaries in northwestern China. The results obtained demonstrated that both bacterial communities shared a similar dominant sub-community, mainly consisting of Actinobacteria, Bacteroidetes, and Proteobacteria, with Limnohabitans and Variovorax as the dominant genera. In spite of these commonalities, bacterial community structures still significantly differed between these two habitats, which may be related to the distance-related dispersal limitation. Their co-occurrence networks were generally both positively structured. The structural analysis showed that OTUs from the same phyla were more likely to co-occur. Although the keystone genera were taxonomically different between Kaidu River and its tributaries, they both shared common trophic properties in exploiting niches under oligotrophic conditions. We noted that their relative abundances were less than 1%, indicating the over-proportional roles of rare genera in the bacterial community. In addition, the inferred networks showed less nodes and edges, but higher modularity in Kaidu River than its tributaries, suggesting the higher fragmentation of the bacterial community in the mainstream.

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“…Polymorphobacter is capable of photosynthetic energy production (Hirose, Matsuura & Haruta, 2016). A previous study of bacterial communities in dust under different lighting conditions suggest that some bacteria can survive in enclosed systems, either as auxotrophs or by cannibalizing other members of the microbial community.…”

Section: Manuscript To Be Reviewedmentioning

confidence: 99%

Peer Review #1 of "Viable bacterial communities on hospital window components in patient rooms (v0.1)"

Ja¹

2020

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Previous studies demonstrate an exchange of bacteria between hospital room surfaces and patients, and a reduction in survival of microorganisms in dust inside buildings from sunlight exposure. While the transmission of microorganisms between humans and their local environment is a continuous exchange which generally does not raise cause for alarm, in a hospital setting with immunocompromised patients, these building-source microbial reservoirs may pose a risk. Window glass is often neglected during hospital disinfection protocols, and the microbial communities found there have not previously been examined. This pilot study examined whether living bacterial communities, and specifically the pathogens Methicillin-resistant Staphylococcus aureus (MRSA) and Clostridioides difficile (C. difficile), were present on window components of exterior-facing windows inside patient rooms, and whether relative light exposure (direct or indirect) impacts bacterial communities on those hospital surfaces. Environmental samples were collected from 30 patient rooms from a single ward at Oregon Health and Science University (OHSU) in Portland, Oregon, USA. Sampling locations within each room included the window glass surface, both sides of the window curtain, two surfaces of the window frame, and the air return grille. Viable bacterial abundances were quantified using qPCR, and community composition was assessed using Illumina MiSeq sequencing of the 16S rRNA gene V3/V4 region. Viable bacteria occupied all sampled locations, but was not associated with a specific hospital surface or relative sunlight exposure. Bacterial communities were similar between window glass and the rest of the room, but had significantly lower Shannon Diversity, theorized to be related to low nutrient density and resistance to bacterial attachment of glass compared to other surface materials. Rooms with windows that were facing west demonstrated a higher abundance of viable bacteria than those facing other directions, potentially because at the time of sampling (morning)

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Phylogenetically Diverse Aerobic Anoxygenic Phototrophic Bacteria Isolated from Epilithic Biofilms in Tama River, Japan

Cited by 27 publications

References 55 publications

Abundance and Spatial Distribution of Aerobic Anoxygenic Phototrophic Bacteria in Tama River, Japan

Abundance and Spatial Distribution of Aerobic Anoxygenic Phototrophic Bacteria in Tama River, Japan

Co-occurrence Network Reveals the Higher Fragmentation of the Bacterial Community in Kaidu River Than Its Tributaries in Northwestern China

Peer Review #1 of "Viable bacterial communities on hospital window components in patient rooms (v0.1)"

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