Phylogenic Study of Twelve Species of Phyllanthus Originated from India through Molecular Markers for Conservation

Rout, Gyana Ranjan; Aparajita, Subhashree

doi:10.4236/ajps.2010.11005

Cited by 3 publications

(1 citation statement)

References 18 publications

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“…They require no prior knowledge of DNA sequence and can amplify a large number of DNA fragments in each reaction. RAPD markers have been successfully used to evaluate relationships at or above the section level in several genera, including Oryza [16], Medicago [17], Rosa [18], Lycopersicon [19], Beta [20], Barbus [21], Citrus [22][23][24], Allium [25], Phyllanthus [26], Asparagus [27], Melon [28] and Amaranth [29]. In each case, the RAPD-derived phylogenies have been found to be in good agreement with those produced using other methods, and most of these reports suggest that RAPD markers are reliable tools for assessment of genetic diversity, analysis of parentage and revealing phylogenetic relationships among various species.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis among some pome fruit trees of Rosaceae family using RAPD markers

Zarei

Erfani‐Moghadam

Mozaffari

2017

Biotechnology & Biotechnological Equipment

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To evaluate the phylogenetic relationships among subtribe Pyrinae of Rosaceae, 50 different genotypes and cultivars of pome fruit trees were collected from various locations in Iran, and analysed using random amplified polymorphic DNA (RAPD) markers. Altogether, 85 polymorphic fragments were produced by 11 random 10-mer primers. The number of produced bands varied from 4 to 12 for each primer, 7.72 on average. The lowest Jaccard's genetic similarity coefficient was scored between apple cv. 'Akan' and a wild Pyrus syriaca pear (0.04), and the highest similarity was observed between two genotypes of P. syriaca (0.97). Cluster analysis using the unweighted pair group method with arithmetic mean (UPGMA) properly separated the accessions and divided them into three major groups, including Malus, Pyrus, Mespilus and Crataegus. Members of Mespilus and Crataegus were further separated and formed two subgroups. Analysis of the genetic structure, using STRUCTURE and phylogenetic relationship using TreeView software confirmed the results of the UPGMA dendrogram and properly distinguished different genera. Among different genera studied in our experiment, Malus was the most distinct one and showed high genetic distance from other genera. Generally, the results from the present study provide valuable information about the genetic relationship among important pome fruit trees in Iran and indicate that, although Mespilus is genetically close to the Crataegus genus, it has relatively high similarity with Pyrus and Malus. Moreover, outcomes of this research confirmed that RAPD molecular markers are an appropriate means for evaluation of the genetic relationship among various genera of pome fruit trees.

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