Phylogenomic investigation of safflower (Carthamus tinctorius) and related species using genotyping-by-sequencing (GBS)

Sardouei-Nasab, Somayeh; Nemati, Zahra; Mohammadi-Nejad, Ghasem; Haghi, Reza; Blattner, Frank R.

doi:10.1038/s41598-023-33347-0

Cited by 5 publications

(2 citation statements)

References 47 publications

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“…The safflower genome is diploid and contains 24 chromosomes [15]. It was recently sequenced with 33,343 gene models predicted [16] and 82,916 gene products annotated [17].…”

Section: Introductionmentioning

confidence: 99%

“…It was recently sequenced with 33,343 gene models predicted [16] and 82,916 gene products annotated [17]. C. tinctorius is the closest relative in the wild, and its single progenitor was C. palaestinus, which was domesticated in the Levant region [15]. Its closest relative with a sequenced genome is the globe artichoke (Cynara cardunculus) [18].…”

Section: Introductionmentioning

confidence: 99%

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Integrated Proteomics and Metabolomics of Safflower Petal Wilting and Seed Development

Vincent,

Reddy,

Isenegger

2024

Biomolecules

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Safflower (Carthamus tinctorius L.) is an ancient oilseed crop of interest due to its diversity of end-use industrial and food products. Proteomic and metabolomic profiling of its organs during seed development, which can provide further insights on seed quality attributes to assist in variety and product development, has not yet been undertaken. In this study, an integrated proteome and metabolic analysis have shown a high complexity of lipophilic proteins and metabolites differentially expressed across organs and tissues during seed development and petal wilting. We demonstrated that these approaches successfully discriminated safflower reproductive organs and developmental stages with the identification of 2179 unique compounds and 3043 peptides matching 724 unique proteins. A comparison between cotyledon and husk tissues revealed the complementarity of using both technologies, with husks mostly featuring metabolites (99%), while cotyledons predominantly yielded peptides (90%). This provided a more complete picture of mechanisms discriminating the seed envelope from what it protected. Furthermore, we showed distinct molecular signatures of petal wilting and colour transition, seed growth, and maturation. We revealed the molecular makeup shift occurring during petal colour transition and wilting, as well as the importance of benzenoids, phenylpropanoids, flavonoids, and pigments. Finally, our study emphasizes that the biochemical mechanisms implicated in the growing and maturing of safflower seeds are complex and far-reaching, as evidenced by AraCyc, PaintOmics, and MetaboAnalyst mapping capabilities. This study provides a new resource for functional knowledge of safflower seed and potentially further enables the precision development of novel products and safflower varieties with biotechnology and molecular farming applications.

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“…The safflower genome is diploid and contains 24 chromosomes [15]. It was recently sequenced with 33,343 gene models predicted [16] and 82,916 gene products annotated [17].…”

Section: Introductionmentioning

confidence: 99%