Phylogeny and morphology of Lasiodiplodia species associated with Magnolia forest plants

Silva, Nimali I. de; Phillips, Alan J. L.; Liu, Jian‐Kui; Lumyong, Saisamorn; Hyde, Kevin D.

doi:10.1038/s41598-019-50804-x

Cited by 35 publications

(32 citation statements)

References 39 publications

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“…[ 5 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 ]. L. pseudotheobromae was also isolated from the leaf and twig of Magnolia candolii as an endophyte and a saprobe [ 19 ]. It has been reported to be associated with citrus stem-end rot disease in Bangladesh and the postharvest fruit rot of lemon in Turkey [ 20 , 21 ].…”

Section: Discussionmentioning

confidence: 99%

“…Due to the wide host range of L. pseudotheobromae , the primary inocula of this disease might also come from other plants in the surrounding area. At the same time, since the fungus has also been found to be an endophyte in several tropical and subtropical trees [ 19 , 22 ], whether it could endogenously grow in citrus trees should also be investigated. Follow-up research is needed to clarify the primary inocula and the inoculum source of the disease.…”

Section: Discussionmentioning

confidence: 99%

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Identification of Lasiodiplodia pseudotheobromae Causing Fruit Rot of Citrus in China

Chen

Zhu

Fù

et al. 2021

Plants

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Considering the huge economic loss caused by postharvest diseases, the identification and prevention of citrus postharvest diseases is vital to the citrus industry. In 2018, 16 decayed citrus fruit from four citrus varieties—Satsuma mandarin (Citrus unshiu), Ponkan (Citrus reticulata Blanco cv. Ponkan), Nanfeng mandarin (Citrus reticulata cv. nanfengmiju), and Sugar orange (Citrus reticulata Blanco)—showing soft rot and sogginess on their surfaces and covered with white mycelia were collected from storage rooms in seven provinces. The pathogens were isolated and the pathogenicity of the isolates was tested. The fungal strains were identified as Lasiodiplodia pseudotheobromae based on their morphological characteristics and phylogenetic analyses using the internal transcribed spacer regions (ITS), translation elongation factor 1-α gene (TEF), and beta-tubulin (TUB) gene sequences. The strains could infect wounded citrus fruit and cause decay within two days post inoculation, but could not infect unwounded fruit. To our knowledge, this is the first report of citrus fruit decay caused by L. pseudotheobromae in China.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Identification of Lasiodiplodia pseudotheobromae Causing Fruit Rot of Citrus in China

Chen

Zhu

Fù

et al. 2021

Plants

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“…Although we could find no reports of these species as endophytes, their relatively recent description makes it likely that their full extent of occurrence has not been revealed. Sequencing phylogenetically informative loci for these endophytic Lasiodiplodia strains–e.g., EF1α and TUB2 ( de Silva et al, 2019 )–will be desirable in the future to confirm their identity with phylogenetic analysis.…”

Section: Discussionmentioning

confidence: 99%

Seed Banks as Incidental Fungi Banks: Fungal Endophyte Diversity in Stored Seeds of Banana Wild Relatives

et al. 2021

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Seed banks were first established to conserve crop genetic diversity, but seed banking has more recently been extended to wild plants, particularly crop wild relatives (CWRs) (e.g., by the Millennium Seed Bank (MSB), Royal Botanic Gardens Kew). CWRs have been recognised as potential reservoirs of beneficial traits for our domesticated crops, and with mounting evidence of the importance of the microbiome to organismal health, it follows that the microbial communities of wild relatives could also be a valuable resource for crop resilience to environmental and pathogenic threats. Endophytic fungi reside asymptomatically inside all plant tissues and have been found to confer advantages to their plant host. Preserving the natural microbial diversity of plants could therefore represent an important secondary conservation role of seed banks. At the same time, species that are reported as endophytes may also be latent pathogens. We explored the potential of the MSB as an incidental fungal endophyte bank by assessing diversity of fungi inside stored seeds. Using banana CWRs in the genus Musa as a case-study, we sequenced an extended ITS-LSU fragment in order to delimit operational taxonomic units (OTUs) and used a similarity and phylogenetics approach for classification. Fungi were successfully detected inside just under one third of the seeds, with a few genera accounting for most of the OTUs–primarily Lasiodiplodia, Fusarium, and Aspergillus–while a large variety of rare OTUs from across the Ascomycota were isolated only once. Fusarium species were notably abundant–of significance in light of Fusarium wilt, a disease threatening global banana crops–and so were targeted for additional sequencing with the marker EF1α in order to delimit species and place them in a phylogeny of the genus. Endophyte community composition, diversity and abundance was significantly different across habitats, and we explored the relationship between community differences and seed germination/viability. Our results show that there is a previously neglected invisible fungal dimension to seed banking that could well have implications for the seed collection and storage procedures, and that collections such as the MSB are indeed a novel source of potentially useful fungal strains.

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“…Botryosphaeriaceae fungi (Ascomycetes, Dothideomycetes) are worldwide distributed as endophytes but mostly as pathogens affecting various crops and forest plants [ 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 ]. As endophytes, they asymptomatically colonise all plant tissues and can be found as saprotrophs on dead plant material.…”

Section: Introductionmentioning

confidence: 99%

“…This endophytic phase may be indeed a latent phase from which many Botryosphaeriaceae species instantly become pathogenic, causing symptoms such as leaf lesions, fruit and root rot, dieback and cankers [ 27 ]. One of the widespread and most studied genera is Lasiodiplodia , comprising many plant-pathogenic species causing yield loss to diverse tropical and even temperate crops [ 20 , 22 , 24 , 25 , 26 , 28 ]. Two species of this genus, L. theobromae and L. pseudotheobromae , are also implicated in human opportunistic infections as keratitis, subcutaneous infections, sinusitis and pneumonia [ 29 , 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Palm Foliage as Pathways of Pathogenic Botryosphaeriaceae Fungi and Host of New Lasiodiplodia Species from Mexico

Douanla‐Meli

Scharnhorst

2021

Pathogens

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Tropical palm foliage is increasingly imported to satisfy the steady growing demand in European floristry. This palm foliage presumably carries along diverse fungi whose taxonomic and functional diversity have not been addressed so far. The present study investigated Botryosphaeriaceae fungi associated with the foliage of palm species Chamaedorea elegans, C. metallica, C. seifrizii, Dypsis lutescens and Lodoicea maldivica imported from Mexico. Five species were identified using combined morphological characterisation and multilocus phylogenetic analyses based on ITS, TEF-1α, TUB2 and RPB2. In addition to Endomelanconiopsis endophytica, Lasiodiplodia brasiliensis and L. euphorbicola, two new species, namely, L. lodoiceae sp. nov. and L. mexicanensis sp. nov, are proposed. Apart from E. endophytica, mostly known as endophyte, L. brasiliensis and L. euphorbicola are responsible for different rot diseases and the dieback of important tropical crop plants. In pathogenicity tests on the temperate pome fruits apple (Malus domestica) and pear (Pyrus communis), all six Botryosphaeriaceae species induced necrotic lesions at different degrees of severity, with highest the aggressiveness from L. euphorbicola and L. mexicanensis on apple and from L. mexicanensis on pear. The results indicate that tropical palm foliage can be a pathway of potentially pathogenic fungi that may give rise to concerns with regard to plant health in the destination countries.

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Phylogeny and morphology of Lasiodiplodia species associated with Magnolia forest plants

Cited by 35 publications

References 39 publications

Identification of Lasiodiplodia pseudotheobromae Causing Fruit Rot of Citrus in China

Identification of Lasiodiplodia pseudotheobromae Causing Fruit Rot of Citrus in China

Seed Banks as Incidental Fungi Banks: Fungal Endophyte Diversity in Stored Seeds of Banana Wild Relatives

Palm Foliage as Pathways of Pathogenic Botryosphaeriaceae Fungi and Host of New Lasiodiplodia Species from Mexico

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