Phylogeny and pathogenicity of <i>Fusarium solani</i> species complex (FSSC) associated with potato tubers

Gherbawy, Youssuf A.; Hussein, Mohamed A.; Hassany, Nabila A.; Shebany, Yassmin M.; Hassan, Sabry; El‐Dawy, Eman G. A.

doi:10.1002/jobm.202100393

Cited by 8 publications

(9 citation statements)

References 45 publications

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“…The isolated strain was grown on a PDA medium and observed by microscope. On the PDA medium, it showed the same phenotype as previously isolated in potato tubers [11]. Aerial mycelia was white at the periphery of the plate.…”

Section: The Morphological Characterization Of the Wn-1 Isolationsupporting

confidence: 72%

“…Therefore, molecular analysis based on DNA information has been widely used to accurately identify and characterize specific pathogenetic species [27]. Previous studies indicated some molecular markers that have been used to identify Fusarium species [8,11,13,28]. For example, the ITS, TEF-1α and β-Tubulin gene sequences are the frequently used markers to identify F. solani isolates [29][30][31].…”

Section: Discussionmentioning

confidence: 99%

“…Pathogens can colonize on both plant living tissue and dead tissue, surviving in the soil as chlamydospores or mycelium [8]. Previous studies showed that many crops, including peas [9], tomatoes and peppers [10], potatoes [11], strawberries [12], Gastrodia elata [13], were heavily infected by F. solani. Some strains can even infect humans and animals [14][15][16][17].…”

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confidence: 99%

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Identification and screening of fungicides against Piper nigrum basal Fusarium wilt disease in Hainan, China

Liu

Chu

et al. 2023

J Basic Microbiol

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Fusarium wilt has occurred in the main Piper nigrum cultivation regions, which seriously affects the yield and quality of P. nigrum. To identify the pathogen of this disease, the diseased roots were collected from a demonstration base in Hainan Province. The pathogen was obtained by tissue isolation method and confirmed by pathogenicity test. Based on the morphological observation, sequence analyses of TEF1‐α nuclear gene, Fusarium solani was identified as the pathogen causing P. nigrum Fusarium wilt and induced symptoms on inoculated plants, including chlorosis, necrotic spots, wilt, drying, and root rot. The experiments for the antifungal activity showed that all the 11 fungicides selected in this study showed certain inhibitory effects on the colony growth of F. solani, where 2% kasugamycin AS, 45% prochloraz EW, 25 g·L−1 fludioxonil SC and 430 g·L−1 tebuconazole SC exhibited relative higher inhibitory effects with EC50 as 0.065, 0.205, 0.395, and 0.483 mg·L−1, respectively, and were selected to perform SEM analysis and test in seeds in vitro. The SEM analysis showed that kasugamycin, prochloraz, fludioxonil, and tebuconazole might have exerted their antifungal effect by damaging F. solani mycelia or microconidia. These preparations were applied as a seed coating of P. nigrum Reyin‐1. The kasugamycin treatment was most effective in reducing the harmful impact of F. solani on the seed germination. These results presented herein provide useful guidance for the effective control of P. nigrum Fusarium wilt.

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Section: The Morphological Characterization Of the Wn-1 Isolationsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Identification and screening of fungicides against Piper nigrum basal Fusarium wilt disease in Hainan, China

Liu

Chu

et al. 2023

J Basic Microbiol

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“…to potato plants varies significantly [ 12 , 19 , 20 ]. The most common pathogenicity assay is the tuber plugging method, which is widely applicable because it allows to obtain unambiguous information on the aggressiveness of strains and reveals the susceptibility of potato cultivars to fungal infection [ 1 , 2 , 3 , 12 , 17 , 21 , 22 , 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Identification and Pathogenicity of Fusarium Fungi Associated with Dry Rot of Potato Tubers

Gavrilova,

Orina,

Trubin

et al. 2024

Microorganisms

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Dry rot of potato tubers is a harmful disease caused by species of the Fusarium genus. Studies on the composition and features of Fusarium spp. that cause the disease in Russia are limited. Thirty-one Fusarium strains belonging to the F. sambucinum species complex (FSAMSC) and F. solani species complex (FSSC) were accurately identified using multilocus phylogenetic analysis of the tef and rpb2 loci, and their physiological characteristics were studied in detail. As a result, 21 strains of F. sambucinum s. str. and 1 strain of F. venenatum within the FSAMSC were identified. Among the analyzed strains within the FSSC, one strain of F. mori, four strains of F. noneumartii, and two strains of both F. stercicola and F. vanettenii species were identified. This is the first record of F. mori on potato as a novel host plant, and the first detection of F. noneumartii and F. stercicola species in Russia. The clear optimal temperature for the growth of the strains belonging to FSAMSC was noted to be 25 °C, with a growth rate of 11.6–15.0 mm/day, whereas, for the strains belonging to FSSC, the optimal temperature range was between 25 and 30 °C, with a growth rate of 5.5–14.1 mm/day. The distinctive ability of F. sambucinum strains to grow at 5 °C has been demonstrated. All analyzed Fusarium strains were pathogenic to potato cv. Gala and caused extensive damage of the tuber tissue at an incubation temperature of 23 °C for one month. Among the fungi belonging to the FSAMSC, the F. sambucinum strains were more aggressive and caused 23.9 ± 2.2 mm of necrosis in the tubers on average compared to the F. venenatum strain—17.7 ± 1.2 mm. Among the fungi belonging to the FSSC, the F. noneumartii strains were the most aggressive and caused 32.2 ± 0.8 mm of necrosis on average. The aggressiveness of the F. mori, F. stercicola, and especially the F. vanettenii strains was significantly lower: the average sizes of damage were 17.5 ± 0.5 mm, 17.2 ± 0.2 mm, and 12.5 ± 1.7 mm, respectively. At an incubation temperature of 5 °C, only the F. sambucinum strains caused tuber necroses in the range of 6.7 ± 0.5–15.9 ± 0.8 mm.

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“…The Fusarium solani species complex (FSSC) comprises 11 clades of fungal species that infect crop roots and cause Fusarium root rot (FRR; Coleman, 2016 ). Species within the FSSC infect a wide range of hosts, including potato, melon, zucchini, soybean, pea, and common bean (Coleman, 2016 ; Medeiros Araújo et al, 2020 ; Roth et al, 2020 ; Díaz‐Nájera et al, 2021 ; Gherbawy et al, 2021 ). Common or dry bean ( Phaseolus vulgaris L.) is an agronomically important staple food for many cultures around the world.…”

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confidence: 99%

An image‐based technique for automated root disease severity assessment using PlantCV

et al. 2023

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Premise Plant disease severity assessments are used to quantify plant–pathogen interactions and identify disease‐resistant lines. One common method for disease assessment involves scoring tissue manually using a semi‐quantitative scale. Automating assessments would provide fast, unbiased, and quantitative measurements of root disease severity, allowing for improved consistency within and across large data sets. However, using traditional Root System Markup Language (RSML) software in the study of root responses to pathogens presents additional challenges; these include the removal of necrotic tissue during the thresholding process, which results in inaccurate image analysis. Methods Using PlantCV, we developed a Python‐based pipeline, herein called RootDS, with two main objectives: (1) improving disease severity phenotyping and (2) generating binary images as inputs for RSML software. We tested the pipeline in common bean inoculated with Fusarium root rot. Results Quantitative disease scores and root area generated by this pipeline had a strong correlation with manually curated values (R2 = 0.92 and 0.90, respectively) and provided a broader capture of variation than manual disease scores. Compared to traditional manual thresholding, images generated using our pipeline did not affect RSML output. Discussion Overall, the RootDS pipeline provides greater functionality in disease score data sets and provides an alternative method for generating image sets for use in available RSML software.

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Phylogeny and pathogenicity of Fusarium solani species complex (FSSC) associated with potato tubers

Cited by 8 publications

References 45 publications

Identification and screening of fungicides against Piper nigrum basal Fusarium wilt disease in Hainan, China

Identification and screening of fungicides against Piper nigrum basal Fusarium wilt disease in Hainan, China

Identification and Pathogenicity of Fusarium Fungi Associated with Dry Rot of Potato Tubers

An image‐based technique for automated root disease severity assessment using PlantCV

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