Phylogeny and Taxonomical Investigation of<i>Trichoderma</i>spp. from Indian Region of Indo-Burma Biodiversity Hot Spot Region with Special Reference to Manipur

Kamala, Th.; Devi, Sarangthem Indira; Sharma, Kusum; Kennedy, Kevin

doi:10.1155/2015/285261

Cited by 43 publications

(26 citation statements)

References 47 publications

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“…Internal transcribed spacer (ITS) identification of Trichoderma spp. is important to verify morphological characterization, since use of morphological identification only had misleading results . Recently, molecular identification based on ITS sequencing has been highly trusted upon .…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and biocontrol efficacy of indigenous Trichoderma isolates against Fusarium wilt of pepper

2019

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Trichoderma species are recognized as biocontrol agents with great potential in inhibiting fungal pathogens that cause significant crop losses. In this study, 15 Trichoderma isolates were collected from various Egyptian locations. Internal transcribed spacer sequencing revealed four different Trichoderma species; Trichoderma harzianum, Trichoderma asperellum, Trichoderma longibrachiatum, and Trichoderma viride. The antagonistic activity of Trichoderma isolates against Fusarium oxysporum f. sp. capsici was evaluated in vitro. The effect of Trichoderma isolates on pepper growth plants in the presence of F. oxysporum was studied in planta. The inhibition of pathogen mycelial growth in vitro ranged between 35.71%and 85.75%. The isolates Ta3 and Tl had the highest antagonistic ability in vitro against F. oxysporum f. sp. capsici. However, Th7 and Th6 of T. harzianum isolates showed the highest values of disease severity reduction under greenhouse conditions. The genetic diversity of the Trichoderma isolates (Ta1, Ta2, Th1, Th2, Th3, Th4, Th5, and Tv) was investigated on the basis of ISSR and SCoT markers. SCoT primers generated a total of 28 bands, out of which 14 (50%) were polymorphic. ISSR primers gave 32 bands, and 11 of these bands (34.37%) were polymorphic. K E Y W O R D Santagonism, Fusarium, genetic diversity, pepper, Trichoderma

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Section: Introductionmentioning

confidence: 99%

Genetic diversity and biocontrol efficacy of indigenous Trichoderma isolates against Fusarium wilt of pepper

2019

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“…The colonial morphology of 5p isolate is white and turned to green at old culture. Because of its macroand microscopic morphology of mycelia and the molecular similarity within Trichoderma genus as described in (30) the isolated fungus can be identified as Trichoderma. harzainum (Fig.…”

Section: Identification and Characterization Of Fungal Isolatesmentioning

confidence: 99%

Bio-removal Efficiency of Glyphosate by Using Indigenous Laccase Producing Fungi

Swe¹,

Nandar²,

Htike³

et al. 2020

IJRASB

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This study was aimed to determine the degradation potential of indigenous fungi isolated from glyphosate contaminated soil. A total number of 10 fungal strains have been isolated and screened their tolerance level against various concentrations of glyphosate (200, 400, 600, 800 and 1000 ppm).These strains were also screened for laccase activity. Only two strains 5p and 6m showed laccase activity and they are characterized, identified and selected for mycoremediation experiment. Due to its macro-and microscopic morphology of mycelia and the molecular similarity, the isolate 5p was identified as Trichoderma harzianum and the isolate 6m as Aspergillus Aiger. 1000ppm of Glyphosate disappeared rapidly in mineral salt liquid media by T.harzianum 78.1% and A.niger 87.3%. Glyphosate almost disappeared by T. harzianum 67.1 % and A. niger 67.2% in the water medium. Based on present findings, these fungal strains can be recommended as potentially effective to protect the environment from the herbicide residues.

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“…could be identifies as T. harzianum T. brevicompactum, T. viride, T. ovalisporum and T. erinaceum H. orientalis and Pachybasium A (T. asperellum). Kamala et al (2015) assessed the genetic diversity of 193 Trichoderma strains from cultivated soils of nine districts of Manipur based on ITS sequences. Among 65 isolates of Trichoderma, 22 different types of Trichoderma spp.…”

Section: Random Amplified Polymorphic Dna (Rapd) Analysismentioning

confidence: 99%

Morphological and Molecular Characterization for Identification of Isolates of <I>Trichoderma</I> spp. from Rhizospheric Soils of Crops in Middle Gujarat

Jaisani¹,

Pandey²

2017

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Trichoderma spp. have gained immense importance as a component of biological control in integrated disease management. Three known isolates and five isolates of Trichoderma spp. collected from rhizospheric soils of tomato, brinjal, sorghum, tobacco and groundnut were characterized morphologically and by molecular tools. The isolates showed significant differences in terms of colony characters, sporulation, branching of conidiophores, phialospores colour and shape. RAPD and ITS analysis showed a comparable output grouping distinctly comprising of known isolate T. viride and isolates of T. viride from brinjal and groundnut in one cluster; known isolate T. harzianum and isolates of T. harzianum from tomato and tobacco in another cluster and known isolate T. virens and T. asperellum from sorghum in single cluster. RAPD analysis of eight isolates showed amplification of 391 bands. Analysis of phylogeny of isolates was done by sequencing the ITS region of ribosomal DNA using specific universal primers ITS 1 and ITS 4. Multiple nucleotide alignment of ITS 1, ITS 2 and 5.8s region depicted intra-specific and inter-specific variations in the ITS sequences among the three isolates of T. harzianum (known isolate), T. harzianum from tomato and tobacco which showed 97 and 83 per cent similarity. However, among the T. viride (known isolate) and T. viride isolates from brinjal and groundnut, per cent similarity was 84 and 95; while, known isolate of T. virens and T. asperellum from sorghum, it was 97 per cent. Thus the study was found useful for identification of Trichoderma spp. from rhizospheric soil of different crops.

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Phylogeny and Taxonomical Investigation ofTrichodermaspp. from Indian Region of Indo-Burma Biodiversity Hot Spot Region with Special Reference to Manipur

Cited by 43 publications

References 47 publications

Genetic diversity and biocontrol efficacy of indigenous Trichoderma isolates against Fusarium wilt of pepper

Genetic diversity and biocontrol efficacy of indigenous Trichoderma isolates against Fusarium wilt of pepper

Bio-removal Efficiency of Glyphosate by Using Indigenous Laccase Producing Fungi

Morphological and Molecular Characterization for Identification of Isolates of <I>Trichoderma</I> spp. from Rhizospheric Soils of Crops in Middle Gujarat

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