Phylogeny and taxonomy of Cladosporium-like hyphomycetes, including Davidiella gen. nov., the teleomorph of Cladosporium s. str.

Braun, Uwe; Crous, Pedro W.; Dugan, Frank M.; Groenewald, J.Z.; Hoog, G.S. de

doi:10.1007/s11557-006-0039-2

Cited by 157 publications

(102 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It should be noted that in a previous analysis of three Cladosporium isolates, the ITS region was not sufficient to identify the species under examination. Braun et al (2003) indicated that assessment of ITS sequences alone does not permit the molecular delimitation of Cladosporium species. Schubert et al (2007) carried out molecular and morphological analyses of the C. herbarum complex, demonstrating that a multilocus approach based on five regions (rDNA ITS, actin, calmodulin, TEF-1α, and histone H3) results in greatly improved resolution, appropriate for species-level identifications.…”

Section: Discussionmentioning

confidence: 99%

Morphological and molecular characterization of Cladosporium cladosporioides species complex causing pecan tree leaf spot

et al. 2016

View full text Add to dashboard Cite

ABSTRACT. The objective of this study was to characterize species of the Cladosporium cladosporioides complex isolated from pecan trees (Carya illinoinensis) with symptoms of leaf spot, based on morphological and molecular approaches. Morphological attributes were assessed using monosporic cultures on potato dextrose agar medium, which were examined for mycelial growth, sporulation, color, and conidia and ramoconidia size. Molecular characterization comprised isolation of DNA and subsequent amplification of the translation elongation factor 1α (TEF-1α) region. Three species of the C. cladosporioides complex were identified: C. cladosporioides, Cladosporium pseudocladosporioides, and Cladosporium subuliforme. Sporulation was the most important characteristic differentiating 2 C. Walker et al. Genetics and Molecular Research 15 (3): gmr.15038714 species of this genus. However, morphological features must be considered together with molecular analysis, as certain characters are indistinguishable between species. TEF-1α can be effectively used to identify and group isolates belonging to the C. cladosporioides complex. The present study provides an important example of a methodology to ascertain similarity between isolates of this complex causing leaf spot in pecan trees, which should facilitate future pathogenicity studies.

show abstract

Section: Discussionmentioning

confidence: 99%

Morphological and molecular characterization of Cladosporium cladosporioides species complex causing pecan tree leaf spot

et al. 2016

View full text Add to dashboard Cite

show abstract

“…of eucalypts with high reproducibility and reliability, a wellcharacterized DNA region which is likely to be highly stable must be targeted. There are more than 30 species of Mycosphaerella on Eucalyptus spp., and related anamorphs increase this number to over 50 (5,6,15,17,18,49). In wheat, the M. graminicola-specific PCR amplifies a fragment of the β-tubulin gene (27).…”

mentioning

confidence: 99%

Development of Nested Polymerase Chain Reaction Detection ofMycosphaerellaspp. and Its Application to the Study of Leaf Disease inEucalyptusPlantations

Glen¹,

Smith²,

Langrell³

et al. 2007

Phytopathology®

View full text Add to dashboard Cite

Glen, M., Smith, A. H., Langrell, S. R. H., and Mohammed, C. L. 2007. Development of nested polymerase chain reaction detection of Mycosphaerella spp. and its application to the study of leaf disease in Eucalyptus plantations. Phytopathology 97:132-144.Mycosphaerella leaf disease (MLD) is a serious disease of two of the major eucalypt species grown in temperate regions worldwide, Eucalyptus globulus and E. nitens. More than 30 species of Mycosphaerella have been reported on eucalypts worldwide. Accurate, rapid, and early discrimination of Mycosphaerella spp. causing crown damage to E. globulus and E. nitens will assist the development of sustainable management strategies. This study describes the development, and incorporation in a nested polymerase chain reaction (PCR) approach, of specific primers for the detection and identification of Mycosphaerella spp. commonly reported from leaf lesions of E. globulus and E. nitens in Australia. Primer design was assisted by sequence alignment and phylogenetic analysis of 165 nonredundant sequences from the nuclear ribosomal DNA internal transcribed spacer regions of Mycosphaerella and related species. Phylogenetic analysis revealed very high sequence similarity for two taxon groups, Mycosphaerella grandis and M. parva, and M. vespa, M. ambiphylla, and M. molleriana, and primers were designed to differentiate each of the two groups. Three other species, M. cryptica, M. nubilosa, and M. tasmaniensis, were distinct and distinguished by species-specific primers. In double-blind trials, the detection test accurately and rapidly identified Mycosphaerella spp. in cultures and discriminated against other pathogens that co-occur in or on Eucalyptus leaves, thereby verifying its reliability. The detection test has an internal amplification control in the first-round PCR with fungal-specific primers to raise confidence in test results, particularly to highlight negative results due to PCR inhibition. When applied to DNA extracted from leaf or stem samples either as multiple or single lesions, it detected and identified up to five Mycosphaerella spp. or taxon groups in both positively identified and in young (putative) MLD lesions. The samples were 20 mm 2 or larger in surface area and were collected while undertaking disease rating assessments in an experimental investigation of Eucalyptus plantations and regrowth forest. Using nested PCR detection, Mycosphaerella spp. were positively identified in 2 days, 1 to 5 months earlier than by classical methods, demonstrating the potential application of this detection test to the early discrimination of MLD components in ecological, epidemiological, and genetic investigations.

show abstract

“…1 Symptoms on Prunus nepalensis leaves showing the progressive stages of leaf blight from initial necrosis at the margins (a) to widespread (b) and then complete necrosis (c) Schubert 2005). Separation of this genus based on these criteria has also been supported by molecular evidence Braun et al 2003). The genus Davidiella was proposed as a teleomorph for Cladosporium anamorphs which were earlier placed in Mycosphaerella sensu lato (Braun et al 2003).…”

mentioning

confidence: 87%

“…Separation of this genus based on these criteria has also been supported by molecular evidence Braun et al 2003). The genus Davidiella was proposed as a teleomorph for Cladosporium anamorphs which were earlier placed in Mycosphaerella sensu lato (Braun et al 2003).…”

mentioning

confidence: 87%

Cladosporium oxysporum on Prunus nepalensis in India

Baiswar

Chandra

Bag

et al. 2011

Australasian Plant Dis. Notes

View full text Add to dashboard Cite

Prunus nepalensis is grown as a fruit crop in Northeast India. Severe leaf blight was observed on almost 40% of seedlings grown in a polyhouse in Meghalaya, India. Light and scanning electron microscopy revealed the presence of Cladosporium oxysporum, which was confirmed by a pathogenicity test. To our knowledge this is the first report of leaf blight on P. nepalensis caused by C. oxysporum in India Keywords Cladosporium oxysporum . Prunus nepalensis Prunus nepalensis is an important fruit crop grown in Northeast India (Patel et al. 2008). Severe leaf blight symptoms were observed on 6-7 months old plants which were growing in a secondary nursery (polybags filled with 1:1:1 soil: sand: farm-yard manure) in a polyhouse at the Horticulture Research Farm of ICAR Research Complex for NEH Region, Umiam, Meghalaya, India. These seedlings were first grown in a primary nursery then moved to a secondary nursery after a month. In most cases, symptoms started at the leaf margins and extended towards the midrib. In the advanced stages of the disease whole leaves turned necrotic and heavy sporulation was evident on the lower surface of the leaves (Fig. 1). Sporulation was rarely seen on the upper leaf surfaces. The necrotic lesions were usually surrounded by a yellow halo (Fig. 2). Heavy defoliation of infected seedlings was also observed. Symptoms were more severe on immature leaves. Seedlings and grafted plants (rootstock-P. nepalensis) were housed in the same polyhouse and grafted plants appeared to be more susceptible. Voucher specimens were deposited in the herbarium collection of MACS Agharkar Research Institute, Pune and ICAR Research Complex for NEH Region, Meghalaya, India (AMH No. 9332, ICARHNEH 138).For pathogen isolation, leaf pieces taken at the edge of lesions with both infected and healthy portions were surface-sterilized using a 4% NaOCl solution for 30 seconds. The sections were washed twice in sterile distilled water and placed onto PDA amended with streptomycin (100 ppm). Four day old isolation cultures were subcultured on Synthetic Nutrient Agar (SNA) at room temperature (21±2°C) and diffused light conditions. Seven days old SNA cultures were used for morphological examination. Fungal structures produced on SNA were removed from the surface of the culture using a strip of Sellotape and mounted in 3% KOH for light microscopy (Correll et al. 1987). A dissecting microscope was used to select suitable areas for scanning electron microscopy (SEM) observations. Leaf areas with abundant sporulation were cut into 5 mm size and placed on double sided Sellotape. Using a JFC-1100 fine coat ion sputter device (JEOL, Tokyo, Japan), samples were sputter coated with gold. Gold-coated samples were then placed on aluminum stubs in a JEOL JSM 6360 scanning electron microscope (JEOL, Tokyo, Japan).For detailed microscopic observations, terms such as primary and secondary ramoconidia, intercalary and terminal conidia were used as described in . Conidiophores were macro-as well as micronematous, also branched in a fe...

show abstract

Phylogeny and taxonomy of Cladosporium-like hyphomycetes, including Davidiella gen. nov., the teleomorph of Cladosporium s. str.

Cited by 157 publications

References 22 publications

Morphological and molecular characterization of Cladosporium cladosporioides species complex causing pecan tree leaf spot

Morphological and molecular characterization of Cladosporium cladosporioides species complex causing pecan tree leaf spot

Development of Nested Polymerase Chain Reaction Detection ofMycosphaerellaspp. and Its Application to the Study of Leaf Disease inEucalyptusPlantations

Cladosporium oxysporum on Prunus nepalensis in India

Contact Info

Product

Resources

About