Phylogeny and variability ofColletotrichum truncatumassociated with soybean anthracnose in Brazil

Abstract

The characterization and distribution of Colletotrichum species in soybean-producing regions in Brazil is fundamental for understanding the disease epidemiology and for ensuring effective control strategies against anthracnose.

Colletotrichumspp. from soybean cause disease on lupin but can also induce plant growth-promoting effects

“…In this study, the interaction of Colletotrichum spec . isolates originating from soybean or lupin were tested for the first time on soybean and lupin cultivars actually used in Germany Since, to our knowledge, so far soybean anthracnose has not yet been systematically investigated on soybean in Germany, we made use of isolates of C. truncatum and C. plurivorum coming from Brazil (Rogério et al 2017; Barbieri et al 2017).…”

“…To get an overview of the virulence spectrum of the different fungal isolates on both host plants, at first, an assay with a simple read-out, namely measurement of lesion lengths, was performed. This method using toothpicks colonized by the fungi was already established and successfully applied for studying Fusarium virguliforme and C. truncatum and C. plurivorum on soybean and applied here for the first time to lupin hypocotyls (Scandiani et al 2011; Rogério et al 2017; Barbieri et al 2017). This assay is very robust and the read-out in form of lesion length measurement is easily quantifiable.…”

“…C. truncatum isolates (see table 1) were provided by N.S. Massola Jr. and (University of São Paulo, ESALQ, Department of Plant Pathology and Nematology, Piracicaba/SP, Brazil) stored at the culture collection of Plant Pathology Department of ‘Luiz de Queiroz’ College of Agriculture, University of São Paulo, Brazil (Rogério et al 2017). Isolates of C. plurivorum (table 1) were also provided by N.S.…”

“…Recently the causal agents of anthracnose on soybean were systematically investigated in Brazil, describing C. truncatum as the major anthracnose-causing species but also identifying C. plurivorum as a novel species capable of causing anthracnose (Rogério et al 2017, 2019; Barbieri et al 2017; Dias et al 2018; Damm et al 2019).…”

Potencial da taxtomina parcialmente purificada no controle de >i<Colletotrichum truncatum>/i< e reação de cultivar de soja ao patógeno

“…Por sua vez, Pascholati et al (2009) (Rogério, 2017). Os fungos foram cultivados em meio de BDA (batata 20% + dextrose 2% + ágar 1,8% + 1000 mL de água destilada), sob fotoperíodo de 12/12 horas (luz/escuro) e temperatura de 23oC, durante 7 dias em câmara tipo B.O.D.…”