Phylogeny of Agaricus subgenus Pseudochitonia with the description of a new section and a new species from Oman

Hussain, Shah; Al-Kharousi, Moza; A., Al-Muharabi, Marwa; Al-Maqbali, Dua’a; Al-Shabibi, Zahra; Al-Balushi, Abdullah H.; Al-Yahya’ei, Mohamed N.; Al, Saady, Nadiya; Abdel‐Jalil, Raid J.; Velazhahan, R.; Al-Sadi, Abdullah M.

doi:10.1007/s11557-022-01819-8

Cited by 7 publications

(5 citation statements)

References 33 publications

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“…Several species of Agaricaceae were recently reported from the Dhofar Region ( Al-Kharousi et al 2022a , 2022b ; Hussain et al 2022 ). It is evident that the area is rich in the diversity of Agaricaceae , including the genus Hymenagaricus .…”

Section: Discussionmentioning

confidence: 99%

“…For ITS amplification, the PCR conditions were optimised as: initial denaturation at 95 °C for 5 min, followed by 35 cycles of denaturation at 95 °C for 30 s, annealing at 54 °C for 45 s and extension at 72 °C for 1 min. For the 28S and EF-1α regions, only the annealing temperature was optimised, 52 °C for 28S and 60 °C EF-1α, respectively ( Hussain et al 2022 ). The PCR products were purified and then sequenced from Macrogen Inc. © (Seoul, Republic of Korea) bidirectionally using the same primers.…”

Section: Materials and Methodsmentioning

confidence: 99%

“…Four species of Agaricaceae , namely Agaricus arabiensis S. Hussain & Al-Sadi, Micropsalliota ventricocystidiata Al-Sadi & S. Hussain, Xanthagaricus appendiculatus Al-Sadi & S. Hussain and X. omanicus Al‐Kharousi, Al‐Sadi & S. Hussain have recently been described from Dhofar Region, Oman ( Al-Kharousi et al 2022a , 2022b ; Hussain et al 2022 ). However, no Hymenagaricus species has been reported from the country.…”

Section: Introductionmentioning

confidence: 99%

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Two new species of Hymenagaricus (Agaricales, Agaricaceae) from Oman, based on morphology and molecular phylogeny

Hussain,

Al-Kharousi,

Al-Maqbali

et al. 2024

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Hymenagaricus has small to medium-sized mushrooms and the cap surface with squamulose pellicles, consisting of hymeniform or pseudoparenchymatous cells and yellowish-brown basidiospores. The species of Hymenagaricus are very similar to those of Xanthagaricus and it is extremely difficult to differentiate the species of both genera in the field. However, phylogenetically, both the genera are clearly distinct. In this study, we describe two new species of Hymenagaricus, i.e. H. wadijarzeezicus and H. parvulus from the southern part of Oman. Species descriptions are based on a combination of morphological characteristics of basidiomata and phylogenetic analyses of three gene regions: internal transcribed spacer (ITS1-5.8S-ITS2 = ITS), the large subunit of nuclear ribosomal DNA (28S) and translation elongation factor one alpha (EF-1α). Full descriptions, micrographs and illustration of anatomical features, basidiomata photos and phylogenetic analyses results of the new taxa are provided. Morphological comparisons of new taxa with similar species and a key to species included in the phylogenetic analyses are also provided.

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Section: Discussionmentioning

confidence: 99%

Section: Materials and Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Two new species of Hymenagaricus (Agaricales, Agaricaceae) from Oman, based on morphology and molecular phylogeny

Hussain,

Al-Kharousi,

Al-Maqbali

et al. 2024

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“…Additionally, Zhao et al (2016) proposed a new taxonomic system that classified Agaricus into five subgenera and 20 sections based on divergence times [2]. In subsequent studies, 2 of 22 new subgenera and sections were established based on the new taxonomic system;presently, Agaricus comprises six subgenera and 27 sections [6,[10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Six New Species of Agaricus (Agaricaceae, Agaricales) from Northeast China

Wang,

Bau

2024

JoF

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Agaricus belongs to Agaricaceae and is one of the most well-known macrofungi, with many edible species. More than 300 Agaricus specimens were collected during a three-year macrofungal resource field investigation in Northeast China. Based on morphological observations and multi-gene (ITS + nrLSU + tef1-a) phylogenetic analyses, six new Agaricus species, i.e., Agaricus aurantipileatus, A. daqinggouensis, A. floccularis, A. griseopileatus, A. sinoagrocyboides, and A. velutinosus, were discovered. These new species belong to four sections within different subgenera: A. (subg. Agaricus) sect. Agaricus, A. (subg. Flavoagaricus) sect. Arvenses, A. (subg. Minores) sect. Minores, and A. (subg. Pseudochitonia) sect. Xanthodermatei. Morphological descriptions, line illustrations, and basidiomata photographs of these new species are provided, and their differences from similar species are discussed.

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“…Sin embargo, hoy en día, los productores de hongos emplean el método de fermentación sólida, la cual es muy lenta, con un alto costo de producción y un bajo rendimiento de biomasa, lo que constituye una de las principales dificultades para su producción a gran escala [1,16]. El desarrollo de procesos de adquisición de micelio en estado sólido está dictado por las propiedades inherentes de estos sistemas heterogéneos, y no es posible tomar muestras durante el desarrollo para determinar las concentraciones correspondientes de biomasa, sustrato y producto [9,15]. Este hecho se refleja de manera particularmente aguda cuando se requieren estudios cinéticos del proceso.…”

Section: Introductionunclassified

Construction of Bioreactors for Obtaining Mycelium from Agaricus Brunnescens and Pleurotus Ostreatus Mushrooms Using the Honey Tek Technique

Ortiz-Bastidas,

Altamirano-Cabay,

Suárez-Tapia

2023

espoch

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Mycelium cultivation plays an important role in mushroom production and in various biotechnological studies. Submerged fermentation has received much attention as a promising alternative for the efficient production of mushroom biomass and active metabolites. For the following research, bioreactors were constructed to obtain pure mycelium from Pleurotus ostreatus and Agaricus brunnescens mushrooms using the Honey technique. This method is based on using honey as the main nutrient for the growth of microorganisms on an industrial scale. In the beginning, spore impressions were extracted by the dry chamber method and inoculated into the honey broth. The bioreactors were dimensioned with a 2.5 L culture chamber, 2 L production, controlled temperature of 28∘C, pH = 4, and agitation of 120 revolutions per minute. Conidia/milliliter was quantified every three days with a Neubauer chamber, and the Kjeldahl method was used to identify protein as the main bioactive metabolite of the mycelium. Finally, the mycelium obtained was evaluated on three types of substrates comparing their colonization time. The use of bioreactors in mycelium cultivation is efficient as better mycelium quality, higher biomass yield, and more dispersion on substrates are obtained. It also has the benefits of much better oxygen mass transfer and culture homogeneity. With this technique, physical, chemical, and biological factors can be controlled to produce mushrooms in a much shorter time. Keywords: bioreactor, honey technic, mycelial biomass, Agaricus brunnescens, Pleurotus ostreatus. Resumen El cultivo de micelio juega un papel importante en la producción de hongos y en diversos estudios biotecnológicos, el usar la fermentación sumergida ha recibido mucha atención como alternativa prometedora para la producción eficiente de la biomasa de hongos y metabolitos activos. Para la siguiente investigación se construyeron biorreactores con el objetivo de obtener micelio puro de hongos Pleurotus ostreatus y Agaricus brunnescens usando la técnica Honey. El método Honey se basa en aprovechar la miel como nutriente principal para el crecimiento de microorganismos a escala industrial. En principio se extraen impresiones de esporas mediante el método de cámara seca y se inoculan en caldo honey. Los biorreactores se dimensionaron con una cámara de cultivo de 2,5 litros, producción de dos litros, temperatura controlada de 28 grados Celsius, pH igual a cuatro y agitación de 120 revoluciones por minuto. Se cuantificaron los conidios/mililitro cada tres días con una cámara de Neubauer y se usó el método Kjeldahl para identificar la proteína como principal metabolito bioactivo del micelio. Finalmente, el micelio obtenido fue evaluado sobre tres tipos de sustratos comparando su tiempo de colonización. El uso de biorreactores en el cultivo de micelio es eficiente debido a que se obtiene mejor calidad de micelio, mayor rendimiento de biomasa y más dispersión sobre sustratos. Así mismo, posee los beneficios de una transferencia de masa de oxígeno y una homogeneidad de cultivo mucho mejores. Con esta técnica se pueden controlar factores físicos, químicos y biológicos para producir setas en tiempos mucho más cortos. Palabras Clave: biorreactor, honey tek, biomasa micelial, Agaricus brunnescens, Pleurotus ostreatus.

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Phylogeny of Agaricus subgenus Pseudochitonia with the description of a new section and a new species from Oman

Cited by 7 publications

References 33 publications

Two new species of Hymenagaricus (Agaricales, Agaricaceae) from Oman, based on morphology and molecular phylogeny

Two new species of Hymenagaricus (Agaricales, Agaricaceae) from Oman, based on morphology and molecular phylogeny

Six New Species of Agaricus (Agaricaceae, Agaricales) from Northeast China

Construction of Bioreactors for Obtaining Mycelium from Agaricus Brunnescens and Pleurotus Ostreatus Mushrooms Using the Honey Tek Technique

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