Phylogeny of Endemic Marine Sponge Hyattella Intestinalis and Characterization of Associated Bacteria

Abstract

Objective: The main objective of this study was to assess the diversity of culturable bacteria associated with marine water sponges. Methods: The marine sponge of Hyattella intestinalis was collected from Sethubavachatram, a village panchayat in the Thanjavur district of Tamil Nadu state, India. One gram of the sponge sample was cut into small pieces and serially diluted. spread on Zobell 2216E marine agar medium and incubated at room temperate (RT, 24±2oC) for 48 h. Based on morphological features, colonies were randomly picked and purified using streak plates. The linked bacteria strains were given names based on the initials of the sponge from which they were recovered. Results: The HIB1, HIB2, and HIB3 were isolated from Hyattella intestinalis. Among the three, HIB1 colonies were found dominant, and further, the biochemical characterization and 16S rRNA sequence was studied. It revealed that the marine sponge Lysinibacillus sp. associated bacteria with HIB1 code. Conclusion: The HIB1 was closely related to Lysinibacillus macroides with 97.81% homology. The NCBI accession number was MZ695813.