Phylogeny of Lens Taxa based on morphological and AFLP markers

Yildizdogan, Zeynep; İkten, Cengiz; Mutlu, Nedim; Toker, Cengiz

doi:10.1016/j.jbiotec.2015.06.081

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“…As it is quite difficult to elucidate the origins of the classical cultivars from old records, molecular biological techniques have been applied to overcome the problem. Amplified fragment length polymorphism (AFLP) analysis has the advantage of both restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD), and can easily detect genetic polymorphisms in the whole genome among accessions, as shown in coconut palm [10], persimmon [11], Hordeum [12,13], Lens [14] and Spiraea [15]. In this study, we conducted AFLP analysis on wabisuke camellias in the two groups and relative Camellia species that have been implicated in the establishment of wabisuke camellias, and investigated the genetic variation and phylogeny of wabisuke camellias.…”

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confidence: 99%

Genetic Variation and Phylogeny of Wabisuke Camellias by Amplified Fragment Length Polymorphism (AFLP) Analysis

Kim

Koike

Nakashima³

et al. 2021

Agronomy

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Amplified fragment length polymorphism (AFLP) analysis was conducted on the wabisuke camellia and its relative camellia species. Genetic polymorphism was identified among the ‘Uraku’ camellia, its offspring ‘Tosa-uraku’ and Camellia japonica, whereas the two accessions of the old ‘Uraku’ showed monomorphism in all the fragments. The results suggested that the two old ‘Uraku’ trees are asexually-propagated clonal strains. The genetic distance between wabisuke cultivars and Chinese camellias and between wabisuke camellias and C. sinensis was much further than that between wabisuke cultivars and Camellia japonica. It has also been suggested that wabisuke camellias can be classified into two subgroups, I-1 and I-2, and that Subgroup I-2 originated from C. japonica, while Subgroup I-1, including ‘Uraku’ (synonym: ‘Tarokaja’), was developed by the repeated hybridization of C. japonica to interspecific hybrids with the Chinese camellias, e.g., C. pitardii var. pitardii, or by the involvement of related species not investigated in this study.

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