Phylogeny of Macroptilium (Leguminosae): morphological, biochemical and molecular evidence

Abstract

Macroptilium (Benth.) Urban (Phaseoleae, Papilionoideae, Leguminosae) is an American genus of legumes, belonging to subtribe Phaseolinae along with other economically important genera, such as Vigna Savi and Phaseolus L. (the common bean genus). Cladistic analyses based on morphological, biochemical (storage seed proteins) and molecular (nuclear and plastid DNA sequences) data were performed on the 18 species currently ascribed to the genus, exploring several character weighting strategies. Equal weights, implied weighting and different transversion ⁄ transition costs were applied. The three data sets were first analyzed with separate partitions, and then combined into a single matrix. This study is the first one to analyze all the species of the genus from a cladistic point of view. In all the most parsimonious trees obtained, Macroptilium is monophyletic with excellent support values. Two monophyletic clades are recovered in almost all the analyses. Both are compound by nine species, and they constitute two sections of Macroptilium. Several interspecific relationships inside the genus are discussed.

Vigna (Leguminosae) sensu lato: The names and identities of the American segregate genera

“…Where paralogous ITS products are commonly amplified in legumes, such as in many mimosoids, they are readily identified as pseudogenes by the numerous small insertion‐deletion regions that occur even in the 5.8S region and by not being GC‐rich (e.g., Bailey et al, 2003; Hughes et al, 2003, 2006). With the exception of certain species of Macroptilium (Espert et al, 2007), we did not detect paralogs in this study, pseudogenes or otherwise. In the case of Macroptilium , we limited sampling to just a few of the common species for which putative ITS orthologs sequenced cleanly and directly from PCR templates (i.e., without polymorphisms or the hallmarks of pseudogenes).…”

Estudio palinológico del género Macroptilium (Benth.) Urb. (Leguminosae) en México y Centroamérica

“…Los dos tipos polínicos encontrados proporcionan elementos a la taxonomía de Macroptilium apoyando la existencia de las secciones anteriormente reconocidas por Drewes (1996) y Torres-Colín (2006), así como la subdivisión en dos secciones sugerida en la hipótesis filogenética propuesta por Espert et al (2007), confirmando la inclusión de Macroptilium gibbosifolium, M. pedatum y M. supinum dentro de la sección Microcochle y la pertenencia de M. atropurpureum, M. erythroloma, M. gracile, M. lathyroides y M. longipedunculatum en la sección Macroptilium.…”

“…A partir de que Macroptilium fue elevado a rango genérico por Urban en 1928, se han publicado más de una docena de nuevas combinaciones y sólo una nueva especie (Maréchal, 1974;Maréchal & Baudet, 1977;Maréchal et al, 1978a;Juárez & Pé-rez, 1986, 1987Drewes & Palacios, 1994Delgado-Salinas & Torres-Colín, 1995, 2004Delgado-Salinas & Lewis, 2008), aunque se ha considerado en algunas revisiones regionales de Brasil (Fevereiro, 1987), Argentina (Drewes, 1997(Drewes, , 2001), Cuba (Beyra & Reyes, 2005), México y Centroamérica (Torres-Colín, 2006), y recientemente en un estudio de datos morfológicos, bioquímicos y moleculares que sustentan la primera propuesta filogenética para el conjunto (Espert et al, 2007).…”

Phylogeny of Prosopis (Leguminosae) as shown by morphological and biochemical evidence

“…Biochemical and molecular analyses have greatly improved the knowledge of the relationships among species of Leguminosae (Burghardt 1996b;Bessega et al 2006;Landeras et al 2006;Espert et al 2007). These tools also show their utility in Prosopis.…”