Phylogeny of Polycladida (Platyhelminthes) based on mtDNA data

Aguado, M. Teresa; Noreña, Carolina; Alcaraz, Lourdes; Marquina, Daniel; Brusa, Francisco; Damborenéa, Cristina; Almón, Bruno; Bleidorn, Christoph; Grande, Cristina

doi:10.1007/s13127-017-0344-4

Cited by 17 publications

(16 citation statements)

References 36 publications

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“…S10,13). We have also used two different variants of MAFFT (Katoh and Standley 2013); previously, MAFFT E-INS-i was selected for the polyclad phylogeny based on mitochondrial sequences (Aguado et al 2017) and for an all-flatworm phylogeny working with the nearly complete nuclear ribosomal marker genes, 18S and 28S (Laumer and Giribet 2017). The other best-scoring 28Sshort6 tree according to our scoring in Table 2 is MAFFT E-INS-i aligned (Suppl.…”

Section: Discussionmentioning

confidence: 99%

“…In two polyclad phylogenies, both BI and ML analyses were run, and the trees show the same or Cotylea in our trees are displayed with blue and red background, respectively. Branches and nodes are given the same colour as their respective taxon topology in Rawlinson et al (2011) and are 'highly congruent' in a mitochondrial gene analysis with several switches within families, but not of the overall topology (Aguado et al 2017). Both models were used to resolve interrelationships within other flatworm orders, and reported with very similar or identical results using combined 18S and 28S datasets (Casu et al 2014;Tessens et al 2014;Janssen et al 2015;Scarpa et al 2015Scarpa et al , 2016Scarpa et al , 2017, in one case also including mitochondrial markers (Janssen et al 2015).…”

Section: Model Choice Is Importantmentioning

confidence: 92%

“…An overview of newly generated and published sequences is provided in Table 1. For most collected material, tissue was stored in 99% ethanol, and histological sections were made as described by Aguado et al (2017) and Dittmann et al (2019). Several published polyclad transcriptomes (Egger et al 2015, Laumer et al 2015 were searched for 18S and 28S sequences (see Table 1) using BLAST (Altschul et al 1990).…”

Section: Animal Collection Identification Of Species and Transcriptomentioning

confidence: 99%

“…In 2017, three large molecular phylogenies of polyclads were published, two with different stretches of the 28S marker gene (Bahia et al 2017;Tsunashima et al 2017), and one with mitochondrial genes (Aguado et al 2017). Of these studies, only Bahia et al dealt with the aforementioned problematic taxa, namely Pericelis, Cestoplana and Theama-all of them showing up as cotyleans in their tree (Bahia et al 2017).…”

Section: Introductionmentioning

confidence: 99%

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Polyclad phylogeny persists to be problematic

et al. 2019

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Two conflicting morphological approaches to polyclad systematics highlight the relevance of molecular data for resolving the interrelationships of Polycladida. In the present study, phylogenetic trees were reconstructed based on a short alignment of the 28S rDNA marker gene with 118 polyclad terminals (24 new) including 100 different polyclad species from 44 genera and 22 families, as well as on a combined dataset using 18S and 28S rDNA genes with 27 polyclad terminals (19 new) covering 26 different polyclad species. In both approaches, Theamatidae and Cestoplanidae were included, two families that have previously been shown to switch from Acotylea to Cotylea. Three different alignment methods were used, both with and without alignment curation by Gblocks, and all alignments were subjected to Bayesian inference and maximum likelihood tree calculations. Over all trees of the combined dataset, an extended majority-rule consensus tree had weak support for Theamatidae and Cestoplanidae as acotyleans, and also the cotylean genera Boninia, Chromyella and Pericelis appeared as acotyleans. With the most inclusive short 28S dataset, on the other hand, there is good support for the aforementioned taxa as cotyleans. Especially with the short 28S matrix, taxon sampling, outgroup selection, alignment method and curation, as well as model choice were all decisive for tree topology. Well-supported parts of the phylogeny over all trees include Pseudocerotoidea, Prosthiostomoidea, Stylochoidea, Leptoplanoidea and Cryptoceloidea, the latter three with new definitions. Unstable positions in the tree were found not only for Theamatidae, Cestoplanidae, Boninia, Chromyella and Pericelis, but also for Anonymus, Chromoplana and Cycloporus.

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Section: Discussionmentioning

confidence: 99%

Section: Model Choice Is Importantmentioning

confidence: 92%

Section: Animal Collection Identification Of Species and Transcriptomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Polyclad phylogeny persists to be problematic

et al. 2019

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“…A fragment of the COI (462 bp) was amplified with the primer pair Hoso_COI_F (5′-ATG GAC GTC CTT TGC GTG AT-3′) and Hoso_COI_R (5′-CAG GAT GTG TTC TAG GAG AGC C-3′). Primer3 online software (http://bioinfo.ut.ee/ primer3/) was used to design these primers, based on the COI sequence of Lurymare clavocapitata Marquina, Aguado, and Noreña, 2015 (GenBank MF371153) (Aguado et al 2017). Polymerase chain reaction (PCR) amplification conditions were 94°C for 5 min; 35 cycles of 94°C for 30 s, 50°C for 30 s, and 72°C for 1 min; and 72°C for 7 min.…”

Section: Methodsmentioning

confidence: 99%

A New Species of <i>Prosthiostomum</i> (Platyhelminthes: Polycladida) from Shirahama, Japan

2019

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We describe a new species of polyclad flatworm, Prosthiostomum torquatum sp. nov., from the rocky intertidal zone in Shirahama, on the Pacific coast of middle Honshu, Japan. Prosthiostomum torquatum is characterised by a unique dorsal colouration, which is comprised of i) numerous orange maculae and blue dots dispersed all over the dorsal surface, ii) a transverse dark-brown line in the anterior part of the body running slightly anterior to another transverse white line, both of which are slightly bent backwards at mid-point, and iii) an incomplete, mesh-like, median band made by dark-brown pigments, fading away posteriorly. By this dorsal colour pattern, the new species can be distinguished from similar congeners such as P. trilineatum Yeri and Kaburaki, 1920 and P. komaii Kato, 1944. We performed a molecular phylogenetic analysis based on 462-bp partial mitochondrial cytochrome c oxidase subunit I (COI) sequences of four species of Prosthiostomidae currently available in public databases in addition to that of P. torquatum. In the resulting tree, P. torquatum was sister to Lurymare clavocapitata Marquina, Aguado, and Noreña, 2015 originally described from Lizard Island, Australia. While these two share a similar dorsal colouration, P. torquatum can be distinguished from L. clavocapitata by i) the absence of a common muscle bulb/sheath enclosing the whole male copulatory apparatus, ii) the median mesh-like band comprised of dark-brown pigments, and iii) COI uncorrected p-distance being 0.094. As a result, our phylogenetic tree indicates the possibility that Prosthiostomum and Lurymare as currently diagnosed may not be monophyletic, and that the common muscle bulb enclosing the whole male copulatory apparatus may not be appropriate as a diagnostic character for Lurymare.

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