Phylogeny of the a genomes of wild and cultivated wheat species

Golovnina, Kseniya; Kondratenko, E. Ya.; Блинов, А. Г.; Гончаров, Н. П.

doi:10.1134/s1022795409110106

Cited by 23 publications

(17 citation statements)

References 19 publications

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“…According to the current concept, the A u and A b genomes diverged approximately one million years ago ( Huang et al 2002 ). Morphologically Triticum urartu and Triticum boeoticum are very similar ( Filatenko et al 2002 ), and differ distinctly only in the leaf pubescence pattern (velvety vs. bristly), controlled by allelic genes ( Golovnina et al 2009 ). These wild species have overlapping distribution ranges, and in some cases accessions belonging to either one of the species are identified incorrectly.…”

Section: Introductionmentioning

confidence: 99%

(GAA)n microsatellite as an indicator of the A genome reorganization during wheat evolution and domestication

Адонина¹,

Гончаров²,

Бадаева³

et al. 2015

CCG

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Although the wheat A genomes have been intensively studied over past decades, many questions concerning the mechanisms of their divergence and evolution still remain unsolved. In the present study we performed comparative analysis of the A genome chromosomes in diploid (Triticum urartu Tumanian ex Gandilyan, 1972, Triticum boeoticum Boissier, 1874 and Triticum monococcum Linnaeus, 1753) and polyploid wheat species representing two evolutionary lineages, Timopheevi (Triticum timopheevii (Zhukovsky) Zhukovsky, 1934 and Triticum zhukovskyi Menabde & Ericzjan, 1960) and Emmer (Triticum dicoccoides (Körnicke ex Ascherson & Graebner) Schweinfurth, 1908, Triticum durum Desfontaines, 1798, and Triticum aestivum Linnaeus, 1753) using a new cytogenetic marker – the pTm30 probe cloned from Triticum monococcum genome and containing (GAA)56 microsatellite sequence. Up to four pTm30 sites located on 1AS, 5AS, 2AS, and 4AL chromosomes have been revealed in the wild diploid species, although most accessions contained one–two (GAA)n sites. The domesticated diploid species Triticum monococcum differs from the wild diploid species by almost complete lack of polymorphism in the distribution of (GAA)n site. Only one (GAA)n site in the 4AL chromosome has been found in Triticum monococcum. Among three wild emmer (Triticum dicoccoides) accessions we detected 4 conserved and 9 polymorphic (GAA)n sites in the A genome. The (GAA)n loci on chromosomes 2AS, 4AL, and 5AL found in of Triticum dicoccoides were retained in Triticum durum and Triticum aestivum. In species of the Timopheevi lineage, the only one, large (GAA)n site has been detected in the short arm of 6At chromosome. (GAA)n site observed in Triticum monococcum are undetectable in the Ab genome of Triticum zhukovskyi, this site could be eliminated over the course of amphiploidization, while the species was established. We also demonstrated that changes in the distribution of (GAA)n sequence on the A-genome chromosomes of diploid and polyploid wheats are associated with chromosomal rearrangements/ modifications, involving mainly the NOR (nucleolus organizer region)-bearing chromosomes, that took place during the evolution of wild and domesticated species.

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Section: Introductionmentioning

confidence: 99%

(GAA)n microsatellite as an indicator of the A genome reorganization during wheat evolution and domestication

Адонина¹,

Гончаров²,

Бадаева³

et al. 2015

CCG

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“…The nuclear genes Acc1 and Pgk1 are predominantly single copy in grasses and encode plastid acetyl-CoA carboxylase and plastid phosphoglycerate kinase, respectively. These two genes, together or separately, have been frequently used for phylogenetic analyses in wheat and other Poaceae (Huang et al 2002a, b;Kilian et al 2007a;Chalupska et al 2008;Golovnina et al 2007Golovnina et al , 2009Fan et al 2007Fan et al , 2009Fan et al , 2012Chen et al 2013;Adderley and Sun 2014).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic relationships among the Iranian Triticum diploid gene pool as inferred from the loci Acc1 and Pgk1

Nasernakhaei¹,

Rahiminejad²,

Saeidi³

et al. 2015

Phytotaxa

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In this study, 129 individuals belonging to the Iranian wild diploid Triticum gene pool were screened using PCR-SSCP based on Acc1 and Pgk1 loci. Sequencing of the representatives of different SSCP patterns allowed for detection of three haplotypes for each of the loci. The phylogenetic relationships among the Iranian wild diploid Triticum haplotypes and related genera were analyzed using DNA sequences from these two single copy loci. Despite previous reports that recommended the usefulness of nuclear single copy genes for phylogenetic inference, the Bayesian and parsimony analyses carried out in this study showed that these two loci were not applicable tools in the phylogeny reconstruction of diploid Triticum gene pool. Thus, our findings confirm that phylogenetic relationships among recently diverged species are often difficult to resolve due to insufficient phylogenetic signal in available markers and/or conflict among gene trees.

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“…3. Alignment of the Pgk-1 gene sequences (from Golovnina et al 2009). Only significant variable sites are shown.…”

Section: Resultsmentioning

confidence: 99%

“…Unfortunately, a single molecular marker suitable for distinguishing each of the diploid species was not found. An investigation of the inheritance of morphological traits also failed to identify species-specific characters in the three diploid wheats (Golovnina et al 2009), except for hairy leaf type which has been previously described (Dorofeev et al 1979).…”

Section: Nuclear Data Setsmentioning

confidence: 99%

“…In our study, available sequences of Acc-1 (plastid acetyl-CoA carboxylase) and Pgk-1 (plastid 3-phosphoglycerate kinase) genes and promoter sequences of the Vrn-1 (MADS-box transcription factor, vernalization gene) gene belonging to different genomes and wheat species were compiled from GenBank and aligned. A number of B, G, and A genome-specific primers pairs were developed based on genome-specific indels (Golovnina et al 2007, 2009, Goncharov et al 2008. One of the A genome-specific primer pairs, Acc-1T(2T) sense/Acc-2T antisense, and the annealing sites are represented in Fig.…”

Section: Nuclear Data Setsmentioning

confidence: 99%

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Taxonomy and molecular phylogeny of natural and artificial wheat species

2009

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The effective use of wheat biodiversity in breeding programs is dependent on a sound conservation strategy for sources of biodiversity, and on appropriate techniques of incorporation into modern cultivars. Producing artificial wheat amphiploids using genomes of related species is an effective way to increase the available gene pool. However, artificial amphiploids should be given botanic names and positions within genus Triticum classification to ensure effective collection and preservation in gene banks. In this review, an attempt to integrate the results of molecular-genetic analyses of natural and artificial wheats with their taxonomy has been made. The correspondence of earlier evolutionary and taxonomic specifications to phylogenetic relationships within Triticum has been estimated using chloroplast and nuclear DNA sequence data. The results indicated close relationships between all artificial and natural species. Based on the data, all wild and cultivated diploid wheat species were united in a separate section, Monococcon. Different variants of nuclear Acc-1, Pgk-1, and Vrn-1 genes have been detected in diploid A genome species. Detailed analysis of the genes showed that one of these variants was a progenitor for all A genomes of polyploid wheats except for that in Triticum zhukovskyi and some of the artificial amphiploids.

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Phylogeny of the a genomes of wild and cultivated wheat species

Cited by 23 publications

References 19 publications

(GAA)n microsatellite as an indicator of the A genome reorganization during wheat evolution and domestication

(GAA)n microsatellite as an indicator of the A genome reorganization during wheat evolution and domestication

Phylogenetic relationships among the Iranian Triticum diploid gene pool as inferred from the loci Acc1 and Pgk1

Taxonomy and molecular phylogeny of natural and artificial wheat species

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