Phylogeny of the filamentous bacterium 'Nostocoida limicola' III from activated sludge.

Liu, Jian-Rong; McKenzie, Christine; Seviour, Elizabeth M.; Webb, Richard I.; Blackall, Linda L.; Saint, Christopher P.; Seviour, Robert J.

doi:10.1099/00207713-51-1-195

Cited by 41 publications

(37 citation statements)

References 30 publications

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“…have been found in foaming activated sludge, let alone Nostocoida Limicola III [17]. Recently, Liu et al [18] and Seviour et al [19] isolated and identified several strains obtained from different wastewater treatment plants in Australia, the Czech Republic, and Italy. They found that the phylogeny of the Nostocoida genus to be very diverse.…”

Section: Foaming Activated Sludge Identification By Non-cultured Basementioning

confidence: 97%

“…The N. limicola I, N. limicola II, and N. limicola III are low G + C content Gram-positive bacteria, high G + C content Gram-positive bacteria, and Planctomycetes, respectively. Liu et al [18] found that the isolates of N. limicola III was Gram-variable with high G + C content bacteria. The N. limicola sp.…”

Section: Foaming Activated Sludge Identification By Non-cultured Basementioning

confidence: 99%

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Filamentous bacteria in a foaming membrane bioreactor

You

Sue

2009

Journal of Membrane Science

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Section: Foaming Activated Sludge Identification By Non-cultured Basementioning

confidence: 97%

Section: Foaming Activated Sludge Identification By Non-cultured Basementioning

confidence: 99%

Filamentous bacteria in a foaming membrane bioreactor

You

Sue

2009

Journal of Membrane Science

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“…1c) (Giovannoni et al, 1987a, b;Lindsay et al, 2001;Liu et al, 2001;Wang et al, 2002;Fuerst, 2005;Ward et al, 2006). Two electron-dense layers separated by an electron-transparent layer were clearly visible in the ultrastructure of the cell wall.…”

mentioning

confidence: 99%

Singulisphaera acidiphila gen. nov., sp. nov., a non-filamentous, Isosphaera-like planctomycete from acidic northern wetlands

Kulichevskaya

Ivanova

Baulina

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

112

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Singulisphaera acidiphila gen. nov., sp. nov., a non-filamentous, Isosphaera-like planctomycete from acidic northern wetlands Four novel strains of budding bacteria, designated MOB10 T , PO2, MPL1015 and BG32, were isolated from acidic wetlands of northern Russia. Cells of these four strains were aerobic, non-motile spheres that occurred singly or in shapeless aggregates and attached to surfaces by means of a holdfast material. The isolates were moderately acidophilic, mesophilic organisms capable of growth between pH 4.2 and 7.5 (optimum growth at pH 5.0-6.2) and at temperatures between 4 and 33 6C (optimum growth at 20-26 6C). The strains possessed a complex intracellular membrane system that compartmentalized the cells. The major fatty acids were C 16 : 0 , C 18 : 1 v9c and C 18 : 2 v6c,12c. The major quinone was menaquinone-6 (MK-6). The G+C content of the DNA was 57.8-59.9 mol%. 16S rRNA gene sequence analysis showed that strains MOB10 T , PO2, MPL1015 and BG32 were members of the order Planctomycetales and belonged to a phylogenetic lineage defined by the genus Isosphaera, exhibiting 90 % sequence similarity to the type strain of the thermophilic planctomycete Isosphaera pallida and 95-95.5 % sequence similarity to a taxonomically uncharacterized group of filamentous bacteria from activated sludge, 'Nostocoida limicola' III. However, compared with 'Nostocoida limicola' III and Isosphaera pallida, the new isolates from acidic wetlands were non-filamentous, unpigmented bacteria, which possessed highly distinctive phospholipid fatty acid profiles and were capable of growth and of degrading several biopolymers under acidic, microaerobic and cold conditions. The data suggest that the four isolates should be considered as representing a novel species of a new genus of the order Planctomycetales, for which the name Singulisphaera acidiphila gen. nov., sp. nov. is proposed. The type strain of Singulisphaera acidiphila is MOB10 T (5ATCC BAA-1392 T 5VKM B-2454 T 5DSM 18658 T ).Members of the order Planctomycetales are ubiquitous in a wide range of terrestrial and aquatic environments with diverse conditions (Staley et al., 1992;Schlesner, 1994;Fuerst, 1995Fuerst, , 2004Ward et al., 2006). We recently showed that these micro-organisms are highly abundant in acidic Sphagnum-dominated northern wetlands Kulichevskaya et al., 2006;Ivanova & Dedysh, 2006). Bacterial cells revealed in Sphagnum peat by means of fluorescence in situ hybridization (FISH) with two phylumspecific 16S rRNA-targeted oligonucleotide probes for planctomycetes, PLA46 and PLA886 (Neef et al., 1998), had ellipsoid or spherical morphology. By using an effective combination of a traditional isolation technique with FISH-based monitoring of the enrichment procedure, we succeeded in isolating several peat-inhabiting, acidophilic planctomycetes in pure culture Kulichevskaya et al., 2006). Three of these isolates were Abbreviations: FISH, fluorescence in situ hybridization; PLFA, phospholipid fatty acid.

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“…Data from laboratory-scale studies repeatedly show that this organism possesses the phenotype expected of a PAO (Pereira et al, 1996;Mino et al, 1998;Hesselmann et al, 2000;Liu et al, 2001). A metabolic model was reconstructed for the dominant Accumulibacter strain (Clade IIA str.…”

Section: Introductionmentioning

confidence: 99%

A metabolic model for members of the genus Tetrasphaera involved in enhanced biological phosphorus removal

Kristiansen

Nguyen²,

Saunders³

et al. 2012

The ISME Journal

206

154

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Members of the genus Tetrasphaera are considered to be putative polyphosphate accumulating organisms (PAOs) in enhanced biological phosphorus removal (EBPR) from wastewater. Although abundant in Danish full-scale wastewater EBPR plants, how similar their ecophysiology is to 'Candidatus Accumulibacter phosphatis' is unclear, although they may occupy different ecological niches in EBPR communities. The genomes of four Tetrasphaera isolates (T. australiensis, T. japonica, T. elongata and T. jenkinsii) were sequenced and annotated, and the data used to construct metabolic models. These models incorporate central aspects of carbon and phosphorus metabolism critical to understanding their behavior under the alternating anaerobic/aerobic conditions encountered in EBPR systems. Key features of these metabolic pathways were investigated in pure cultures, although poor growth limited their analyses to T. japonica and T. elongata. Based on the models, we propose that under anaerobic conditions the Tetrasphaerarelated PAOs take up glucose and ferment this to succinate and other components. They also synthesize glycogen as a storage polymer, using energy generated from the degradation of stored polyphosphate and substrate fermentation. During the aerobic phase, the stored glycogen is catabolized to provide energy for growth and to replenish the intracellular polyphosphate reserves needed for subsequent anaerobic metabolism. They are also able to denitrify. This physiology is markedly different to that displayed by 'Candidatus Accumulibacter phosphatis', and reveals Tetrasphaera populations to be unusual and physiologically versatile PAOs carrying out denitrification, fermentation and polyphosphate accumulation.

show abstract

Phylogeny of the filamentous bacterium 'Nostocoida limicola' III from activated sludge.

Cited by 41 publications

References 30 publications

Filamentous bacteria in a foaming membrane bioreactor

Filamentous bacteria in a foaming membrane bioreactor

Singulisphaera acidiphila gen. nov., sp. nov., a non-filamentous, Isosphaera-like planctomycete from acidic northern wetlands

A metabolic model for members of the genus Tetrasphaera involved in enhanced biological phosphorus removal

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