Phylogeny of the order Scytosiphonales (Phaeophyceae) based on DNA sequences of <i>rbc</i>L, partial <i>rbc</i>S, and partial LSU nrDNA

Kogame, Kazuhiro; Horiguchi, Takeo; Masuda, Michio

doi:10.2216/i0031-8884-38-6-496.1

Cited by 117 publications

(110 citation statements)

References 12 publications

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“…still differs by only two bp from Korean P. latifolia. The two bp difference (-0.14%) can be due to isolation by distance and falls well within the intraspecific variation for this gene in brown algae (Kogame et al 1999, Cho et al 2003, Cho and Boo 2006. P. plantaginea (Roth) Greville (AF055410, Greenland) differs by 28 bp (1.91%) from both Hecatonema.…”

Section: Claveiformis Is Probablysupporting

confidence: 77%

Life history and morphological studies ofPunctaria tenuissima(Chordariaceae, Phaeophyceae), a new record for the Azores

Parente¹,

Fletcher²,

Neto³

et al. 2010

Botm

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Punctaria tenuissima (Chordariaceae, Phaeophyceae) is reported for the first time from the Azores. Erect thalli were collected on the Island of São Miguel; they were up to 3 cm long, flattened and often twisted. The plurilocular sporangia were formed from surface cells that were quadrate or rectangular in surface view. Unilocular sporangia were not observed in the field. In culture, the plurispores of P. tenuissima developed into Hecatonema-like, tufted, prostrate thalli that formed plurilocular sporangia. The plurispores of the prostrate thalli cultured at 158C with a long day (LD) photoperiod developed into new prostrate thalli, which in turn formed plurilocular sporangia. This cycle was repeated 5 times, resulting in several generations of reproductive prostrate thalli. When these cultures were cooled to 108C with a short day (SD) photoperiod, new erect thalli developed from the prostrate thalli resembling the thalli collected in the field. These erect blades produced unilocular and plurilocular sporangia on the same or on different thalli. The plurispores and unispores produced by the erect thalli at 108C under SD conditions once again developed into new prostrate thalli. This is the first report of unilocular sporangia formed in cultures of P. tenuissima. Sexual reproduction was not observed. The culture conditions, particularly temperature and daylength, appeared to influence the formation of erect blades. The Punctaria-like thalli were produced in 108C/SD conditions, whilst the Hecatonema-like thalli were produced under 108-158C/LD and SD conditions. These results are similar to those reported for P. tenuissima from other locations and suggest the occurrence of both direct and heteromorphic life histories in the Azorean algae.

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Section: Claveiformis Is Probablysupporting

confidence: 77%

Life history and morphological studies ofPunctaria tenuissima(Chordariaceae, Phaeophyceae), a new record for the Azores

Parente¹,

Fletcher²,

Neto³

et al. 2010

Botm

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“…The purified DNA was used as template DNA in a polymerase chain reaction (PCR) to amplify the ITS2 region, the partial rbcL-spacer-partial rbcS (rbcL-sp-S) region and the mitochondrion-encoded cox3 (cytochrome oxidase subunit 3) gene. Kogame et al, 1999; for reverse) for the rbcLsp-S region; CAF4A (5 0 -ATGTTTACTTGGTGRA GRGA-3 0 ; this study; for forward) and CAR4A (5 0 -CCCCACCARTAWATNGTNAG-3 0 ; this study; for reverse) for the cox3 gene. PCR and sequencing were carried out as described by Kogame et al (1999) except that the annealing temperature was 45 C for PCR of the cox3.…”

mentioning

confidence: 80%

“…Kogame et al, 1999; for reverse) for the rbcLsp-S region; CAF4A (5 0 -ATGTTTACTTGGTGRA GRGA-3 0 ; this study; for forward) and CAR4A (5 0 -CCCCACCARTAWATNGTNAG-3 0 ; this study; for reverse) for the cox3 gene. PCR and sequencing were carried out as described by Kogame et al (1999) except that the annealing temperature was 45 C for PCR of the cox3.…”

mentioning

confidence: 80%

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A study of sexual and asexual populations ofScytosiphon lomentaria(Scytosiphonaceae, Phaeophyceae) in Hokkaido, northern Japan, using molecular markers

Kogame

Uwai

Shimada

et al. 2005

European Journal of Phycology

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The presence or absence of gamete fusion in the dioecious and isogamous brown alga Scytosiphon lomentaria (Scytosiphonaceae, Phaeophyceae) was investigated for thalli collected from three localities, Oshoro, Asari and Muroran in Hokkaido, northern Japan. Both sexual and asexual thalli were found in each locality. These sexual (35 samples) and asexual thalli (15 samples) were used to establish unialgal cultures. ITS2 sequences were determined in all cultures, the rbcL-spacer-S in 14, and cox3 in 25 cultures. Thirteen haplotypes (A1-4, B, C1-4, D, E1-3) were found in ITS2 sequences (241-252 bp). Ten haplotypes (A1-4, B, C1-4 and D) were found in sexual samples: haplotypes A and B were found in Oshoro, C and D in Asari, and C in Muroran. Haplotypes E1-3 were found in asexual samples. Sequence divergence values (including gap information) were less than 4.03% among sexual samples (A, B, C and D types), 0.38-0.77% among asexual samples (E types) and 4.39-5.70% between sexual and asexual samples. In the mitochondrial cox3 gene region (543 bp), six haplotypes (K1-3, L, M and N) were obtained. Cox3-K types were found in samples of ITS2-A types, cox3-L type in ITS2-B type, cox3-M type in ITS2-C and D types, and cox3-N type in ITS2-E types. For cox3 sequences, nucleotide differences were 0.18-4.42% among sexual samples (K, L and M types), but 8.66-10.31% between sexual and asexual samples (N type). In partial rbcL (174 bp)-spacer (188 bp)-partial rbcS (90 bp) sequences, six haplotypes (R-W) were found although there were 1-4 bp nucleotide differences among these haplotypes. R, U and W types were found in sexual samples, and S, T and V types in asexual samples. Results of the sequence analyses suggest that our presumed asexual thalli are genetically different from sexual thalli and may be derived from populations that lack sexual reproduction.

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“…Culture conditions used were 5°C SD (short day, 8:16 h light:dark regime), 10°C SD, 10°C LD (long day, 16:8 h light:dark regime), 15°C SD, 15°C LD, 20°C SD, 20°C LD and 25°C LD, under 30-50 μmol m -2 s -1 . Total genomic DNA was extracted from cultured thalli and purified as previously described by Kogame et al (1999). The purified DNA was used as template DNA for PCR to amplify the rbcL and cox1 genes.…”

Section: Methodsmentioning

confidence: 99%

Two species of the genus Acinetospora (Ectocarpales, Phaeophyceae) from Japan: A. filamentosa comb. nov. and A. asiatica sp. nov.

et al. 2015

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Phylogeny of the order Scytosiphonales (Phaeophyceae) based on DNA sequences of rbcL, partial rbcS, and partial LSU nrDNA

Cited by 117 publications

References 12 publications

Life history and morphological studies ofPunctaria tenuissima(Chordariaceae, Phaeophyceae), a new record for the Azores

Life history and morphological studies ofPunctaria tenuissima(Chordariaceae, Phaeophyceae), a new record for the Azores

A study of sexual and asexual populations ofScytosiphon lomentaria(Scytosiphonaceae, Phaeophyceae) in Hokkaido, northern Japan, using molecular markers

Two species of the genus Acinetospora (Ectocarpales, Phaeophyceae) from Japan: A. filamentosa comb. nov. and A. asiatica sp. nov.

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