Phylogeny, topology, structure and functions of membrane-bound class III peroxidases in vascular plants

Lüthje, Sabine; Meisrimler, Claudia-Nicole; Hopff, David; Möller, Benjamin

doi:10.1016/j.phytochem.2010.11.023

Cited by 35 publications

(38 citation statements)

References 101 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The role of gene 19544 was analyzed in detail, as its expression might affect the quality and quantity of phenolics. (Valério et al, 2004;Mathé et al, 2010;Lüthje et al, 2011). Therefore, it is important to determine the expression pattern of PRX27 to understand the physiological roles and characteristics.…”

Section: Microarray Analysismentioning

confidence: 99%

“…As a consequence of the large number of genes and the two possible catalytic cycles, it is assumed that class III plant peroxidases are involved in a broad range of physiological processes like cell elongation, cell wall differentiation, or defense. They function in cell wall metabolism (Marjamaa et al, 2009), wound healing, auxin catabolism, removal of H 2 O 2 , oxidation of toxic reductants, defense against pathogen and insect attack, as well as symbiosis and normal cell growth (Cosio and Dunand, 2009;Lüthje et al, 2011). Their roles in the modification of cell wall structures may include suberin polymerization, cross linking of the structural nonenzymatic proteins such as expansins, catalyzing the formation of diferulic acid linkages between polysaccharide-bound lignin or ferulic acid residues in polysaccharides, and the production of hydroxyl radicals with the ability to cleave cell wall polymers (Marjamaa et al, 2009).…”

Section: Faprx27mentioning

confidence: 99%

“…The basic protein (calculated pI of 8.47) harbors a predicted signal peptide for the secretory pathway (amino acids 1-26), a predicted transmembrane region (amino acids 7-29), six predicted N-glycosylation sites (amino acids 82-87, 95-100, 151-156, 168-173, 209-214, and 216-221), and assumed phosphorylation sites (http://elm.eu.org/search/; Dinkel et al, 2012). Most of the putative membrane-bound PRXs were predicted as secretory proteins (Lüthje et al, 2011). Besides cleavable N-terminal signal peptides, transmembrane domains have been calculated by several prediction programs for a number of class III peroxidases.…”

Section: Faprx27mentioning

confidence: 99%

See 2 more Smart Citations

Metabolic Interaction between Anthocyanin and Lignin Biosynthesis Is Associated with Peroxidase FaPRX27 in Strawberry Fruit

et al. 2013

View full text Add to dashboard Cite

Plant phenolics have drawn increasing attention due to their potential nutritional benefits. Although the basic reactions of the phenolics biosynthetic pathways in plants have been intensively analyzed, the regulation of their accumulation and flux through the pathway is not that well established. The aim of this study was to use a strawberry (Fragaria × ananassa) microarray to investigate gene expression patterns associated with the accumulation of phenylpropanoids, flavonoids, and anthocyanins in strawberry fruit. An examination of the transcriptome, coupled with metabolite profiling data from different commercial varieties, was undertaken to identify genes whose expression correlated with altered phenolics composition. Seventeen comparative microarray analyses revealed 15 genes that were differentially (more than 200-fold) expressed in phenolics-rich versus phenolics-poor varieties. The results were validated by heterologous expression of the peroxidase FaPRX27 gene, which showed the highest altered expression level (more than 900-fold). The encoded protein was functionally characterized and is assumed to be involved in lignin formation during strawberry fruit ripening. Quantitative trait locus analysis indicated that the genomic region of FaPRX27 is associated with the fruit color trait. Down-regulation of the CHALCONE SYNTHASE gene and concomitant induction of FaPRX27 expression diverted the flux from anthocyanins to lignin. The results highlight the competition of the different phenolics pathways for their common precursors. The list of the 15 candidates provides new genes that are likely to impact polyphenol accumulation in strawberry fruit and could be used to develop molecular markers to select phenolics-rich germplasm.

show abstract

Section: Microarray Analysismentioning

confidence: 99%

Section: Faprx27mentioning

confidence: 99%

Section: Faprx27mentioning

confidence: 99%

See 1 more Smart Citation

Metabolic Interaction between Anthocyanin and Lignin Biosynthesis Is Associated with Peroxidase FaPRX27 in Strawberry Fruit

et al. 2013

View full text Add to dashboard Cite

show abstract

“…The major peroxidases present in plant ECS are of class III, for which more than 100 genes were found in plant genomes. By phylogenetic analysis several class III peroxidases can be identified, as well as their promoters [90,91,92,93]. While producing information on specific functions and localizations, it is difficult to define specific functions for each form due to their low substrate specificity in the in vitro assays [94].…”

Section: The Biological Processes Detected In the Ecsmentioning

confidence: 99%

Protein Dynamics in the Plant Extracellular Space

et al. 2016

View full text Add to dashboard Cite

The extracellular space (ECS or apoplast) is the plant cell compartment external to the plasma membrane, which includes the cell walls, the intercellular space and the apoplastic fluid (APF). The present review is focused on APF proteomics papers and intends to draw information on the metabolic processes occurring in the ECS under abiotic and biotic stresses, as well as under non-challenged conditions. The large majority of the proteins detected are involved in “cell wall organization and biogenesis”, “response to stimulus” and “protein metabolism”. It becomes apparent that some proteins are always detected, irrespective of the experimental conditions, although with different relative contribution. This fact suggests that non-challenged plants have intrinsic constitutive metabolic processes of stress/defense in the ECS. In addition to the multiple functions ascribed to the ECS proteins, should be considered the interactions established between themselves and with the plasma membrane and its components. These interactions are crucial in connecting exterior and interior of the cell, and even simple protein actions in the ECS can have profound effects on plant performance. The proteins of the ECS are permanently contributing to the high dynamic nature of this plant compartment, which seems fundamental to plant development and adaptation to the environmental conditions.

show abstract

“…According to the NCBI data base, 13) these values and peptides are typical of the class III plant secretory peroxidase family. 14,15) Not surprisingly, polypeptides found to be common to the Aloe and Arabidopsis peroxidases include sequences (A-L-RL-FHDCFV, TG-GEIR) (Fig. 3) that are conserved in most plant peroxidases.…”

Section: Proteomic Characterizationmentioning

confidence: 99%

Proteomic Identification of a Basic Peroxidase Stabilized within Acetylated Polymannan Polysaccharide of <i>Aloe barbadensis</i>

Vittori¹,

Martı́n

Sabater

2012

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

Phylogeny, topology, structure and functions of membrane-bound class III peroxidases in vascular plants

Cited by 35 publications

References 101 publications

Metabolic Interaction between Anthocyanin and Lignin Biosynthesis Is Associated with Peroxidase FaPRX27 in Strawberry Fruit

Metabolic Interaction between Anthocyanin and Lignin Biosynthesis Is Associated with Peroxidase FaPRX27 in Strawberry Fruit

Protein Dynamics in the Plant Extracellular Space

Proteomic Identification of a Basic Peroxidase Stabilized within Acetylated Polymannan Polysaccharide of <i>Aloe barbadensis</i>

Contact Info

Product

Resources

About