Phylogeographic analysis reveals northerly refugia for the riverine amphibian<i>Triturus dobrogicus</i>(Caudata: Salamandridae)

Vörös, Judit; Mikulíček, Peter; Major, Ágnes; Recuero, Ernesto; Arntzen, Jan W.

doi:10.1111/bij.12866

Cited by 34 publications

(36 citation statements)

References 88 publications

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“…The size of the symbols is proportional to the observed haplotype frequency in Triturus cristatus (red), T. dobrogicus (purple), and T. carnifex (orange). Numbers indicate either the ID of individuals sequenced in this study or GenBank entries (JNxx) originally published by Vörös et al ()…”

Section: Resultsmentioning

confidence: 99%

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Species‐specific habitat preferences do not shape the structure of a crested newt hybrid zone (Triturus cristatus x T. carnifex)

Mačát

Rulı́k

Jablonski

et al. 2019

Ecology and Evolution

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Reproductive isolation barriers maintain the integrity of species by preventing interspecific gene flow. They involve temporal, habitat or behavioral isolation acting before fertilization, and postzygotic isolation manifested as hybrid mortality or sterility. One of the approaches of how to study reproductive isolation barriers is through the analysis of hybrid zones. In this paper, we describe the structure of a hybrid zone between two crested newt species (Triturus cristatus and T. carnifex) in the southern part of the Czech Republic using morphological, microsatellite, and mitochondrial (mtDNA) markers. Specifically, we tested the hypothesis that the structure of the hybrid zone is maintained by species‐specific habitat preferences. Comparing the genetic structure of populations with geographical and ecological parameters, we found that the hybrid zone was structured primarily geographically, with T. cristatus‐like populations occurring in the northeast and T. carnifex‐like populations in the southwest. Despite T. cristatus tending to occur in deeper ponds and T. carnifex on localities with more shading, the effect of both ecological parameters on the structure of the zone was minimal. Next, we corroborated that T. carnifex individuals and some hybrids possess mtDNA of T. dobrogicus, whose nuclear background was not detected in the studied hybrid zone. Hybridization between T. carnifex and T. dobrogicus (resulting in unidirectional mtDNA introgression) had to predate subsequent formation of the hybrid zone between T. cristatus and T. carnifex. Populations of crested newts in the southern part of the Czech Republic thus represent a genetic mosaic of nuclear and mitochondrial genomes of three species.

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Section: Resultsmentioning

confidence: 99%

“…In order to find out the origin of T. dobrogicus mtDNA, we compared haplotypes from the Znojmo region with haplotypes from the whole range of T. dobrogicus (Vörös, Mikulíček, Major, Recuero, & Arntzen, ). Haplotypes of T .…”

Section: Resultsmentioning

confidence: 99%

Species‐specific habitat preferences do not shape the structure of a crested newt hybrid zone (Triturus cristatus x T. carnifex)

Mačát

Rulı́k

Jablonski

et al. 2019

Ecology and Evolution

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“…3,000‐km‐long hybrid zone coiled over central Europe, with B. bombina in the lowlands and B. variegata in hills and mountains. Strong biogeographical evidence exists for a scenario of species displacement, in which B. bombina superseded B. variegata over large parts of central Europe, until a stationary position was encountered at the transition of lowlands and mountain foothills with habitat‐dependent species performance as the driving force for hybrid zone movement (Arntzen, ; Vörös, Mikulíček, Major, Recuero, & Arntzen, ). Similarly, any parapatric species contact zone may travel the landscape.…”

Section: Discussionmentioning

confidence: 99%

Hybrid zone formation and contrasting outcomes of secondary contact over transects in common toads

Arntzen

Vries²,

Canestrelli³

et al. 2017

Molecular Ecology

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Much progress in speciation research stems from documenting patterns of morphological and genetic variation in hybrid zones. Contrasting patterns of marker introgression in different sections of the contact can provide valuable insights on the relative importance of various evolutionary mechanisms maintaining species differences in the face of hybridization and gene flow and on hybrid zone temporal and spatial dynamics. We studied species interactions in the common toads Bufo bufo and B. spinosus in France and northwestern Italy using morphological and molecular data from the mitochondrial and nuclear genomes in an extensive survey, including two independent transects west and east of the Alps. At both, we found sharp, coincident and concordant nuclear genetic transitions. However, morphological clines were wider or absent and mtDNA introgression was asymmetric. We discuss alternative, nonexclusive hypotheses about evolutionary processes generating these patterns, including drift, selection, long-distance dispersal and spatial shifts in hybrid zone location and structure. The distribution of intraspecific mtDNA lineages supports a scenario in which B. bufo held a local refugium during the last glacial maximum. Present-day genetic profiles are best explained by an advance of B. spinosus from a nearby Iberian refugium, largely superseding the local B. bufo population, followed by an advance of B. bufo from the Balkans, with prongs north and south of the Alps, driving B. spinosus southwards. A pendulum moving hybrid zone, first northwards and then southwards, explains the wide areas of introgression at either side of the current position of the contact zones.

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“…Zeisset and Beebee ; Vörös et al. ). Forward primers were labelled with fluorescent dyes (Fam‐6, NED, PET or VIC), reverse primers contained a GTTT pigtail sequence on their 5′ end to increase the accuracy of genotyping.…”

Section: Methodsmentioning

confidence: 99%

“…Microsatellites have proven to have the potential in resolving recent (at least since the last glacial maxima) evolutionary history of amphibians (e.g. Zeisset and Beebee 2001;V€ or€ os et al 2016). Forward primers were labelled with fluorescent dyes (Fam-6, NED, PET or VIC), reverse primers contained a GTTT pigtail sequence on their 5 0 end to increase the accuracy of genotyping.…”

Section: Sampling and Laboratory Analysesmentioning

confidence: 99%

Increased genetic structuring of isolatedSalamandra salamandrapopulations (Caudata: Salamandridae) at the margins of the Carpathian Mountains

Vörös

Ursenbacher

Kiss

et al. 2016

J Zool Syst Evol Res

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The traditional southern Pleistocene refugia hypothesis in Europe has lately been challenged for several animal and plant species. The Carpathian Basin, especially at the marginal regions, is one of the recently recognized biodiversity hotspots in Europe. Marginal populations are prone to have lower genetic diversity and higher genetic differentiation than central populations. Here, we examined one mitochondrial DNA fragment (D‐loop) and nine nuclear (microsatellite) loci to describe the genetic diversity and phylogeographical pattern of fire salamander (Salamandra salamandra) populations in the Carpathian Basin with focusing on the southern margins of the Western Carpathians, where isolated populations of this species are present. Analyses of microsatellites indicated reduced genetic diversity for most of the isolated populations. Based on the mitochondrial DNA, only two haplotypes were found, whereas the analyses with the nuclear markers revealed a more recent genetic split between Western (Alpine) and Eastern (Carpathian) populations, and separated the Apuseni Mountains population (part of the Western Carpathians). Using approximate Bayesian computation analyses, we identified the most probable colonization scenario for the isolated North Hungarian Carpathian Basin populations. The split between isolated salamander populations from the central populations in the Carpathian Mountains dates back to the beginning of the Late Pleistocene, while the split between most of the Hungarian populations can be associated with the Last Glacial Maximum. We found evidence for long‐time isolation between the marginal Carpathian Basin and central populations. Our results also show that S. salamandra survived glacial periods in the temperate forests of north‐east Pannonia (North Hungarian Mountains), confirming that the Carpathian Basin served as important northerly refugia during the Pleistocene climatic oscillations.

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Phylogeographic analysis reveals northerly refugia for the riverine amphibianTriturus dobrogicus(Caudata: Salamandridae)

Cited by 34 publications

References 88 publications

Species‐specific habitat preferences do not shape the structure of a crested newt hybrid zone (Triturus cristatus x T. carnifex)

Species‐specific habitat preferences do not shape the structure of a crested newt hybrid zone (Triturus cristatus x T. carnifex)

Hybrid zone formation and contrasting outcomes of secondary contact over transects in common toads

Increased genetic structuring of isolatedSalamandra salamandrapopulations (Caudata: Salamandridae) at the margins of the Carpathian Mountains

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