Phylogeographic relationships within the Mediterranean turbot inferred by mitochondrial DNA haplotype variation

Abstract

The Mediterranean turbot Psetta maxima consists of two main genetically distinct lineages (western Mediterranean and 'eastern secluded Mediterranean' basins) as investigated by mitochondrial DNA analysis. Within the latter lineage, most haplotypes from the Sea of Azov were endemic and more than half of them derived from a single ancestral haplotype shared among all the eastern Mediterranean areas. There was no relation between morphotype variation in bony tubercles and mitochondrial genealogy. # 2004 The Fisheries Society of the British Isles

Mitochondrial Control Region DNA Variation in Turbot Populations from the Bulgarian and Romanian Black Sea Coasts

“…the determined Kimura 2-parameter /K2P/ interhaplotype genetic distances ranged from zero to 0.017 (Se = 0.007), with an average value equal to 0.008 (Se = 0.002). the maximum and average values of the K2P genetic distance calculated for the studied haplotypes were significantly lower than those for the 'Pmax' set of turbot cR haplotypes reported by Suzuki et al (16), (K2P max = 0.044, Se = 0.011; K2P avr = 0.014, Se = 0.003). the 'Pmax' set resembles turbot samples collected from a geographically broader area including the Atlantic ocean, western and eastern Mediterranean and related seas.…”

“…Dover sole (15), Japanese flounder (6) and willowy flounder (21). Despite the observed very low interhaplotype genetic distance the present study showed a high level of haplotype diversity with an average value of 0.47 (36 haplotypes for 76 analyzed samples) versus 0.42 (28 haplotypes for 66 turbot samples) for the Pmax haplotypes, Suzuki et al (16). the main reason is that the majority of the identified haplotypes originate from a small set of dominant haplotypes following single nucleotide substitution.…”

“…taken together the results from these studies suggest that turbot populations are related to high gene flow rate combined with low level of adaptive polymorphisms and genetic differentiation for some coastal areas. An important result from the studies of turbot populations along the european coastal areas is the observed relatively low genetic variability in comparison to other flatfish species, suggesting historically closer bottlenecks along the turbot evolution (2,16). the earlier turbot studies, which were based on morphological characterization, proposed distinct differentiation between and within the studied population and existence of turbot sub-species (19).…”

“…the earlier turbot studies, which were based on morphological characterization, proposed distinct differentiation between and within the studied population and existence of turbot sub-species (19). however, Suzuki et al (16) found no relation between morphotype variation in bony tubercles and mitochondrial genealogy. Within the same study the authors proposed that the Mediterranean turbot populations belong to two main genetically distinct lineages, 'western Mediterranean' and 'eastern secluded Mediterranean basins'.…”

“…in europe, the species inhabits wide coastal areas from the northeast Atlantic and Baltic Sea down to the Mediterranean and the Black Sea. the local turbot populations were studied using a wide range of morphological parameters, biochemical (3,7,18) and molecular markers (2,11,16,20). Several recent studies of natural turbot populations demonstrated a weak genetic structure among the populations from the northeast Atlantic and the low but significant genetic differentiation of turbot from the Baltic Sea (5,12,20).…”