Physcomitrella patens auxin conjugate synthetase (GH3) double knockout mutants are more resistant to Pythium infection than wild type

Mittag, Jennifer; Šola, Ivana; Rusak, Gordana; Ludwig‐Müller, Jutta

doi:10.1016/j.jplph.2015.05.015

Cited by 24 publications

(28 citation statements)

References 45 publications

(74 reference statements)

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“…Similar studies were reported that auxin influx carrier LAX supported vascular bundle development [18,42]. In addition, the active state of IAA was inactivated by GH3 genes [26,43]. Further our results proved that the PBZ treatment might change the auxin pathway genes activity to reduce IAA content, and could affect the distribution of transverse vascular bundles, cell division and cell elongation on the surface fruits, thus regulating the bulged surface in pear fruits.…”

Section: Pbz Reduced the Content Of Iaa Bulged Sruface Of Fruitssupporting

confidence: 88%

Comparative analysis of transcrptome profiling to identify genes involved in bulged surface of pear fruits (Pyrus bretschneideri Rehd. cv. Yuluxiangli)

Ding

Song

et al. 2020

Preprint

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Background: Pear (Pyrus spp.) belongs to the genus Pyrus, in the family Rosaceae. Some varieties of pear fruits exhibit bulged surface, which seriously affect the quality and commodity value. In this study, we performed anatomical, physiological and transcriptomic analysis, to explore the mechanism of paclobutrazol (PBZ) on the bulged surface of pear fruits. Results: The vascular bundles of flesh were more evenly distributed and the cells arranged more compactly with smaller size. However, the auxin (IAA) content of flesh decreased significantly when treated with PBZ. Further, the GO and KEGG analysis of differentially expressed genes (DEGs) showed that auxin, phenylpropanoid metabolic pathways and transcriptional factor genes were significantly enriched on the relieved bulged surface of pear fruits. The promoter region analysis of selected DEGs showed that some genes contained auxin responded cis-elements. Conclusion: We conclude that PBZ might play a negative role in the cell division, cell elongation and vascular bundle development on bulged surface of pear fruits through the involvement of auxin related genes. This study will provide theoretical basis for the regulation of bulged surface of pear fruits by growth retardant agent.

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Section: Pbz Reduced the Content Of Iaa Bulged Sruface Of Fruitssupporting

confidence: 88%

Comparative analysis of transcrptome profiling to identify genes involved in bulged surface of pear fruits (Pyrus bretschneideri Rehd. cv. Yuluxiangli)

Ding

Song

et al. 2020

Preprint

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“…In P. patens , auxin levels increase in P.c. carotovorum elicitor-treated and Pythium -infected tissues (Mittag et al, 2015; Alvarez et al, 2016). Furthermore, the use of a moss reporter line harboring the auxin inducible promoter GH3 (indole-3-acetic acid amino acid conjugate synthetases) fused to β-glucuronidase, shows that auxin signaling is induced in response to P.c.…”

Section: Functional Evolution Of Hormonal Pathways and Their Role In mentioning

confidence: 99%

“…Furthermore, the use of a moss reporter line harboring the auxin inducible promoter GH3 (indole-3-acetic acid amino acid conjugate synthetases) fused to β-glucuronidase, shows that auxin signaling is induced in response to P.c. carotovorum elicitors and the pathogens P. irregulare , P. debaryanum , and C. gloeosporoides (Mittag et al, 2015; Reboledo et al, 2015; Alvarez et al, 2016). Like in A. thaliana where auxin induces the expression of genes and activates defense, auxin has been associated to P. patens defense against pathogens (Mittag et al, 2015).…”

Section: Functional Evolution Of Hormonal Pathways and Their Role In mentioning

confidence: 99%

“…carotovorum elicitors and the pathogens P. irregulare , P. debaryanum , and C. gloeosporoides (Mittag et al, 2015; Reboledo et al, 2015; Alvarez et al, 2016). Like in A. thaliana where auxin induces the expression of genes and activates defense, auxin has been associated to P. patens defense against pathogens (Mittag et al, 2015). High auxin levels present in P. patens GH3 double knockout plants increase resistance against P. debaryanum (Mittag et al, 2015), and auxin induces the expression of the defense gene PAL.1 (Reboledo et al, 2015).…”

Section: Functional Evolution Of Hormonal Pathways and Their Role In mentioning

confidence: 99%

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Adaptation Mechanisms in the Evolution of Moss Defenses to Microbes

León

Montesano

2017

Front. Plant Sci.

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Bryophytes, including mosses, liverworts and hornworts are early land plants that have evolved key adaptation mechanisms to cope with abiotic stresses and microorganisms. Microbial symbioses facilitated plant colonization of land by enhancing nutrient uptake leading to improved plant growth and fitness. In addition, early land plants acquired novel defense mechanisms to protect plant tissues from pre-existing microbial pathogens. Due to its evolutionary stage linking unicellular green algae to vascular plants, the non-vascular moss Physcomitrella patens is an interesting organism to explore the adaptation mechanisms developed in the evolution of plant defenses to microbes. Cellular and biochemical approaches, gene expression profiles, and functional analysis of genes by targeted gene disruption have revealed that several defense mechanisms against microbial pathogens are conserved between mosses and flowering plants. P. patens perceives pathogen associated molecular patterns by plasma membrane receptor(s) and transduces the signal through a MAP kinase (MAPK) cascade leading to the activation of cell wall associated defenses and expression of genes that encode proteins with different roles in plant resistance. After pathogen assault, P. patens also activates the production of ROS, induces a HR-like reaction and increases levels of some hormones. Furthermore, alternative metabolic pathways are present in P. patens leading to the production of a distinct metabolic scenario than flowering plants that could contribute to defense. P. patens has acquired genes by horizontal transfer from prokaryotes and fungi, and some of them could represent adaptive benefits for resistance to biotic stress. In this review, the current knowledge related to the evolution of plant defense responses against pathogens will be discussed, focusing on the latest advances made in the model plant P. patens.

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“…P. patens responds to pathogens, including fungi, oomycetes, and bacteria, by activating defenses including production of reactive oxygen species (ROS), cell wall fortifications, hormonal perturbations, and expression of defenserelated genes (Lehtonen et al, 2012;Mittag et al, 2015;Oliver et al, 2009;Ponce de León et al, 2007. However, PTI components have not been identified in P. patens or other bryophytes.…”

Section: Introductionmentioning

confidence: 99%

An Innate Immunity Pathway in the Moss Physcomitrella patens

et al. 2016

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MAP kinase (MPK) cascades in Arabidopsis thaliana and other vascular plants are activated by developmental cues, abiotic stress, and pathogen infection. Much less is known of MPK functions in nonvascular land plants such as the moss Physcomitrella patens. Here, we provide evidence for a signaling pathway in P. patens required for immunity triggered by pathogen associated molecular patterns (PAMPs). This pathway induces rapid growth inhibition, a novel fluorescence burst, cell wall depositions, and accumulation of defense-related transcripts. Two P. patens MPKs (MPK4a and MPK4b) are phosphorylated and activated in response to PAMPs. This activation in response to the fungal PAMP chitin requires a chitin receptor and one or more MAP kinase kinase kinases and MAP kinase kinases. Knockout lines of MPK4a appear wild type but have increased susceptibility to the pathogenic fungi Botrytis cinerea and Alternaria brassisicola. Both PAMPs and osmotic stress activate some of the same MPKs in Arabidopsis. In contrast, abscisic acid treatment or osmotic stress of P. patens does not activate MPK4a or any other MPK, but activates at least one SnRK2 kinase. Signaling via MPK4a may therefore be specific to immunity, and the moss relies on other pathways to respond to osmotic stress.

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Physcomitrella patens auxin conjugate synthetase (GH3) double knockout mutants are more resistant to Pythium infection than wild type

Cited by 24 publications

References 45 publications

Comparative analysis of transcrptome profiling to identify genes involved in bulged surface of pear fruits (Pyrus bretschneideri Rehd. cv. Yuluxiangli)

Comparative analysis of transcrptome profiling to identify genes involved in bulged surface of pear fruits (Pyrus bretschneideri Rehd. cv. Yuluxiangli)

Adaptation Mechanisms in the Evolution of Moss Defenses to Microbes

An Innate Immunity Pathway in the Moss Physcomitrella patens

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