Elevated blood pressure (BP) during childhood and adolescence increases the risk of hypertension in later life. Although physical activity is known to positively moderate BP, data regarding this relationship are limited in prepubertal children. We aimed to assess the association between a range of physical activities (including indoor and outdoor activity) and BP in a large community-based sample of prepubertal schoolchildren. Eligible year-1 schoolchildren (n¼1765; mean age 6.7 ± 0.4 years) from a random cluster sample of 34 schools in Sydney, Australia, were examined. Parents completed detailed questionnaires about their child's activity. Height and weight were measured, and body mass index (BMI) was calculated. BP was measured using a standard protocol, and elevated BP was defined using published guidelines. Physical activity was classified as low, medium or high (that is, as tertiles). After adjusting for age, sex, ethnicity, height, BMI, parental qualifications and family history of hypertension and/or cardiovascular disease, children in the highest tertile of outdoor and indoor activities had significantly lower diastolic BP (B1.5 mm Hg; P trend ¼0.01) and systolic BP (B1.3 mm Hg; P trend ¼0.03), respectively, compared with those in the lowest tertile (reference). Linearly, time spent in indoor activities (each hour per day) was associated with B2.4 mm Hg decrease in diastolic BP (P¼0.001). Physical activity was independently associated with lower BP in this sample of prepubertal children. The findings emphasize the importance of ensuring regular physical activity programs in primary schools to potentially reduce the risk of elevated BP in childhood and in later life. Keywords: blood pressure; children; physical activity; prepuberty; Sydney Childhood Eye Study INTRODUCTION Elevated blood pressure (BP) during childhood and adolescence is associated with increased risk of hypertension and cardiovascular disease (CVD), 1 and the development of early pathological lesions of atherosclerosis. 2,3 Thus, the recognition and management of elevated BP at an early age, and the identification of modifiable risk factors, may be an important strategy for limiting the overall public health disease burden caused by hypertension. 4,5 Physical activity is a key component of the therapeutic lifestyle changes recommended for preventing and treating elevated BP and hypertension in children and adolescence. 6,7 There is evidence that levels of physical activity, aerobic fitness and CVD risk factors track from childhood and adolescence into adulthood. 8 A number of observational studies have investigated associations between physical activity and BP in children and adolescents, 9-12 but the findings from these studies have been equivocal. Recently, a UK study of 5505 children aged 11-12 years showed that higher levels of physical activity were associated with lower BP, and the authors concluded that the volume of activity may be more important than the intensity. 10 In contrast, several studies reported no or a positive associat...