Physical and Biochemical Characteristics of Biological Fluids in Rats with Modeled Thermal Injury

Abstract

Lactate dehydrogenase activity, crystallogenic and initiator characteristics of biological substrates were evaluated by enzymological and crystalloscopic analysis of rat serum and urine. Changes in these characteristics of biological media in combined thermal injury were shown. This approach is effective for evaluation of the metabolic status in rats with experimental burn disease.

Estimation of Effectiveness of Local Reactive Oxygen Species for Stimulation of Regenerative Processes in Experimental Burn Wound

“…Under ether anesthesia, all animals were once subjected to thermal trauma with the help of an experimental installation for applying a contact thermal burn. As a result, a contact thermal burn of the III degree was inflicted on the dorsal part, which is 20% of the entire skin surface [25][26][27]. The verification of the depth of the burn was carried out by us in previous studies on the basis of histological examination of wound tissue in the dynamics of the wound process [25][26][27].…”

“…As a result, a contact thermal burn of the III degree was inflicted on the dorsal part, which is 20% of the entire skin surface [25][26][27]. The verification of the depth of the burn was carried out by us in previous studies on the basis of histological examination of wound tissue in the dynamics of the wound process [25][26][27]. Animals' euthanasia was realized with use of lethal dose of anesthetics.…”

“…The experiments were carried out on 45 white Wistar rats weighing 160-180 g, randomized into 3 groups. Under ether anesthesia, thermal trauma was inflicted on all animals-a contact thermal burn of the III degree of the posterior surface of the body on an area of 20% of body square [25][26][27]. The animals of the first group (control) had their burn wounds treated daily with Levomecol; in the second group, with ozonated oil (OOM) [10,12]; in the third group, an ozone-oxygen gas mixture was subcutaneously injected into the edges of the wounds and the wounds were treated with Levomecol [25][26][27][28].…”

Влияние Динитрозильных Комплексов Железа На Параметры Окислительного Метаболизма При Экспериментальной Термической Травме

“…Èçâåñòíî, ÷òî îaeîãîâàÿ áîëåçíü ïðåäñòàâëÿåò ñîáîé êîìïëåêñíóþ îòâåòíóþ ðåàêöèþ ïðàêòè÷åñêè âñåõ ñèñòåì îðãàíèçìà íà çíà÷èìóþ òåðìè÷åñêóþ òðàâìó [1 -6]. Ñèñòåìíîñòü âîçíèêàþùèõ ïðè ôîðìèðîâàíèè äàííîé ïàòîëîãèè íàðóøåíèé ïðåäóñìàòðèâàåò ðàçâèòèå îêèñëèòåëüíîãî ñòðåññà (ÎÑ), íàëè÷èå êîòîðîãî ïðè îaeîãîâîé áîëåçíè ïðîäåìîíñòðèðîâàíî ðàíåå íàìè è äðóãèìè èññëåäîâàòåëÿìè [2,7,8].  ñâîþ î÷åðåäü, ÎÑ, ñóùåñòâåííî ñíèaeàþùèé àäàïòàöèîííûé ïîòåíöèàë îðãàíèçìà, â òîì ÷èñëå â óñëîâèÿõ òåðìè÷åñêîé òðàâìû, ñïîñîáåí îêàçûâàòü íåãàòèâíîå âëèÿíèå íà ñêîðîñòü ðåãåíåðàòèâíûõ ïðîöåññîâ, òåìïû êóïèðîâàíèÿ ýíäîãåííîé èíòîêñèêàöèè è òå÷åíèå îaeîãîâîé áîëåçíè â öåëîì [5,9,10].…”

“…Ýêñïåðèìåíòû ñ èñïîëüçîâàíèåì ýêñïåðèìåíòàëüíûõ aeèâîòíûõ ïðîâîäèëèñü ñ ñîáëþäåíèåì ïðèíöèïîâ ãóìàííîñòè, èçëîaeåííûõ â äèðåêòèâàõ Åâðîïåéñêîãî ñîîáùåñòâà (¹ 86/609/ÅÅÑ, Ñòðàñáóðã, 1986). Áûëî ñôîðìèðîâàíî 3 ãðóïïû aeèâîòíûõ: ïåðâóþ (èíòàêòíóþ) ãðóïïó ñîñòàâèëè 10 êðûñ, ñ êîòîðûìè íå ïðîâîäèëè íèêàêèõ ìàíèïóëÿöèé; âî âòîðîé (êîíòðîëüíîé) è òðåòüåé (îïûòíîé) ãðóïïàõ aeèâîòíûì (n = 10 â êàaeäîé) ìîäåëèðîâàëè òÿaeåëóþ òåðìè÷åñêóþ òðàâìó, âêëþ÷àâøóþ êîíòàêòíûé îaeîã íà ïëîùàäè 20 % ïîâåðõíîñòè òåëà è òåðìîèíãàëÿöèîííîå ïîðàaeåíèå, ïî ñîáñòâåííîé ìåòîäèêå [8], çàòåì åaeåäíåâíî îñóùåñòâëÿëè ìåñòíîå ëå÷åíèå îaeîãà äî âûâåäåíèÿ aeèâîòíûõ èç ýêñïåðèìåíòà. Êðîìå òîãî, êðûñàì êîíòðîëüíîé è îïûòíîé ãðóïïû åaeåäíåâíî âíóòðèáðþøèííî ââîäèëè ïî 3 ìë èçîòîíè÷åñêîãî ðàñòâîðà õëîðèäà íàòðèÿ, äëÿ aeèâîòíûõ òðåòüåé ãðóïïû äîïîëíèòåëüíî ñîäåðaeàùèé 0,3 ììîëü/ë ÄÍÊAE ñ ãëóòàòèîíîâûìè ëèãàíäàìè (îäíîêðàòíàÿ äîçà ñîåäèíåíèÿ -2,86 ìêã/ã ìàññû aeèâîòíîãî; ïî [13].…”