Physical and Biological Characterization of Superparamagnetic Iron Oxide- and Ultrasmall Superparamagnetic Iron Oxide-Labeled Cells

Sun, Rui; Dittrich, J; Le-Huu, Martin; Mueller, Margareta M.; Bedke, Jens; Kartenbeck, Juergen; Lehmann, Wolf D.; Krueger, Ralf; Bock, Michael; Huss, Ralf; Seliger, Christian; Gröne, Herrmann Josef; Misselwitz, Bernd; Semmler, Willi; Kießling, Fabian

doi:10.1097/01.rli.0000162925.26703.3a

Cited by 88 publications

(83 citation statements)

References 31 publications

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“…Results revealed a positive induction within 7 days, demonstrated by staining of cartilage-specific proteogylcans. A mean cellular iron content of 24 pg was found in co-labeled MSC, this finding was similar to reported values for magnetic cell labeling without toxic effects [10,41,43]. Therefore, it was assumed that the co-labeling approach described above was an applicable method to study MSC in-vivo by MRI and to validate their localization by histology.…”

Section: Discussionsupporting

confidence: 82%

“…Furthermore, labeling approaches vary in terms of cell incubation and the possible use of transfection agents [9]. All the different magnetic labeling techniques result in a considerable increase of the cellular iron content up to 100 pg per cell [7] compared to a physiological cellular iron content of generally less than 1 pg [10]. The large amount of intracellular iron oxide particles and the use of high-resolution T2*-weighted gradient echo sequences permits cellular MRI techniques to be very sensitive.…”

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confidence: 99%

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Magnetic Resonance Imaging of Single Co-Labeled Mesenchymal Stromal Cells after Intracardial Injection in Mice

Salamon

Wicklein

Didié

et al. 2014

Fortschr Röntgenstr

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Ziel: Etablierung einer Doppelmarkierung mesenchymaler Stromazellen (MSZ) zur in-vivo Detektion einzelner MSZ mittels MRT und zur histologischen Validierung. Material und Methoden: Murine MSZ wurden mit fluoreszierenden Eisenmikropartikeln und Carboxyfluorescein Succinimidyl Ester (CFSE) markiert. Der zelluläre Eisengehalt wurde mittels Atomabsorptionsspektrometrie bestimmt. Zellprolieferation und Expression charakteristischer Oberflächenmarker wurden mittels Durchflusszytometrie bestimmt. Die chondrogene Differenzierungskapazität wurde überprüft. Verschiedene Zellanzahlen (n1 = 5000, n2 = 15 000, n3 = 50 000) wurden bei 12 Mäusen in den linken Herzventrikel injiziert. Es erfolgte die sequenzielle MRT der Tiere an einem klinischen 3.0 T MRT. Zur histologischen Validierung wurden Kryostatschnitte fluoreszensmikroskopisch untersucht. Ergebnisse: Die magnetische und fluoreszierende Doppelmarkierung von MSZ wurde etabliert (mittlerer zellulärer Eisengehalt 23,6 ± 4,3 pg). Durchflusszytometrisch zeigten sich ähn-liche Zellprolieferationsraten und Rezeptorexpressionsprofile von markierten und unmarkierten MSZ. Die chondrogene Differenzierung der doppelt markierten MSZ wurde verifiziert. Nach Zellinjektion zeigten sich im MRT multiple Signalauslöschungen im Hirn und geringer in der Niere. Im Hirn fanden sich durchschnittlich 4,6 ± 1,2 (n1), 9,0 ± 3,6 (n2) und 25,0 ± 1,0 (n3) Signalauslöschungen pro Schicht. Durchschnittlich fanden sich 8,7 ± 3,1 (n1), 22,0 ± 6,1 (n2) und 89,8 ± 6,5 (n3) Zellen pro korrespondierendem Kryostatschnitt. Die statistische Korrelation der mittels MRT detektierten Signalauslöschungen und der histologisch nachgewiesenen Zellen ergab einen Korrelationskoeffizienten von r = 0,91. Schlussfolgerung: Die Studie zeigt die erfolgreiche magnetische und fluoreszierende DoppelmarkieAbstract ! Purpose: The aim of this study was to establish co-labeling of mesenchymal stromal cells (MSC) for the detection of single MSC in-vivo by MRI and histological validation. Materials and Methods: Mouse MSC were co-labeled with fluorescent iron oxide micro-particles and carboxyfluorescein succinimidyl ester (CFSE). The cellular iron content was determined by atomic absorption spectrometry. Cell proliferation and expression of characteristic surface markers were determined by flow cytometry. The chondrogenic differentiation capacity was assessed. Different amounts of cells (n1 = 5000, n2 = 15 000, n3 = 50 000) were injected into the left heart ventricle of 12 mice. The animals underwent sequential MRI on a clinical 3.0 T scanner (Intera, Philips Medical Systems, Best, The Netherlands). For histological validation cryosections were examined by fluorescent microscopy. Results: Magnetic and fluorescent labeling of MSC was established (mean cellular iron content 23.6 ± 3 pg). Flow cytometry showed similar cell proliferation and receptor expression of labeled and unlabeled MSC. Chondrogenic differentiation of labeled MSC was verified. After cell injection MRI revealed multiple signal voids in the brain and fewer signal voids...

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Section: Discussionsupporting

confidence: 82%

mentioning

confidence: 99%

Magnetic Resonance Imaging of Single Co-Labeled Mesenchymal Stromal Cells after Intracardial Injection in Mice

Salamon

Wicklein

Didié

et al. 2014

Fortschr Röntgenstr

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“…Particles may also penetrate cells by still other mechanisms (Rothen-Rutishauser et al 2006). The efficiency of nanoparticle incorporation is known to depend on size (Rejman et al 2004;Chithrani et al 2006), shape (Chithrani et al 2006), surface charge (Labhasetwar et al 1998;Arbab et al 2003;Oyewumi et al 2004;Sun et al 2005) and surface chemistry (Labhasetwar et al 1998;Holzapfel et al 2006;Lorenz et al 2006;Nativo et al 2008) of the particles.…”

Section: Cellular Uptake Of Fept Nanoparticlesmentioning

confidence: 99%

Quantitative analysis of the protein corona on FePt nanoparticles formed by transferrin binding

Jiang

Weise

Hafner

et al. 2009

J. R. Soc. Interface.

204

206

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Nanoparticles are finding a rapidly expanding range of applications in research and technology, finally entering our daily life in medical, cosmetic or food products. Their ability to invade all regions of an organism including cells and cellular organelles offers new strategies for medical diagnosis and therapy (nanomedicine), but their safe use requires a deep knowledge about their interactions with biological systems at the molecular level. Upon incorporation, nanoparticles are exposed to biological fluids from which they adsorb proteins and other biomolecules to form a 'protein corona'. These nanoparticle -protein interactions are still poorly understood and quantitative studies to characterize them remain scarce. Here we have quantitatively analysed the adsorption of human transferrin onto small (radius approx. 5 nm) polymer-coated FePt nanoparticles by using fluorescence correlation spectroscopy. Transferrin binds to the negatively charged nanoparticles with an affinity of approximately 26 mM in a cooperative fashion and forms a monolayer with a thickness of 7 nm. By using confocal fluorescence microscopy, we have observed that the uptake of FePt nanoparticles by HeLa cells is suppressed by the protein corona compared with the bare nanoparticles.

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“…Recently, a number of reports described labeling and tracking of cells through the use of SPIO nanoparticles or fluorescent dyes (Soriano et al, 1992;Hill et al, 2003;Kraitchman et al, 2003;Werner et al, 2003;Bos et al, 2004;Kong et al, 2004a,b;Corti et al, 2005;Sun et al, 2005Sun et al, , 2008Ju et al, 2007). Moreover, the small animal in vivo imaging systems permit sensitive and quantitative spatial monitoring of markers in whole animals.…”

Section: Discussionmentioning

confidence: 99%

In Vivo Tracking of Dual‐Labeled Mesenchymal Stem Cells Homing Into the Injured Common Carotid Artery

Cao

Shi

Zhang

et al. 2009

The Anatomical Record

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The aim of this study is to conduct in vivo, noninvasive magnetic resonance imaging of labeled rat bone mesenchymal stem cells (BMSCs) as they home into the site of injured common carotid artery following allograft transplantation. Our study was approved by the Institutional Committee on Animal Research. Purified rat BMSCs were dual labeled with superparamagnetic iron oxide (SPIO) particle and fluorescent DiI dye, and subsequently transplanted into recipient rats injured in the left common carotid arteries. Immediately before and 3 hr, 3, 7 and 12 days after transplantation, the labeled cells were monitored in vivo using a 7T micromagnetic resonance imaging (7T micro-MRI) scanner. The signal-tonoise ratios (SNRs) at the injured sites were corroborated with histological examination using Prussian blue staining and fluorescent imaging. Rat BMSCs were labeled with SPIO and DiI at 100% efficiency. When compared with the baseline level before transplantation, the SNR decreased significantly on Days 3 and 7 after injection in the experimental group (Dunnet t test, P < 0.05), whereas insignificant differences were observed after 3 hr and 12 days (Dunnet t test, P > 0.05). In the control group, no significant differences in SNR were found among different time points (ANOVA, P > 0.05). Histological analyses illustrated that red fluorescence and Prussian blue-positive cells were mainly distributed around the lesion areas of injured common carotid arteries. Rat BMSCs can be efficiently labeled with SPIO and DiI, and the directional homing of labeled cells to the site of injured common carotid arteries after intravascular transplantation could be tracked in vivo with 7T micro-MRI. Anat

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Physical and Biological Characterization of Superparamagnetic Iron Oxide- and Ultrasmall Superparamagnetic Iron Oxide-Labeled Cells

Cited by 88 publications

References 31 publications

Magnetic Resonance Imaging of Single Co-Labeled Mesenchymal Stromal Cells after Intracardial Injection in Mice

Magnetic Resonance Imaging of Single Co-Labeled Mesenchymal Stromal Cells after Intracardial Injection in Mice

Quantitative analysis of the protein corona on FePt nanoparticles formed by transferrin binding

In Vivo Tracking of Dual‐Labeled Mesenchymal Stem Cells Homing Into the Injured Common Carotid Artery

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