Physical and genetic analyses of streptococcal plasmid pAM beta 1 and cloning of its replication region

LeBlanc, Donald J.; Lee, L N

doi:10.1128/jb.157.2.445-453.1984

Cited by 104 publications

(23 citation statements)

References 34 publications

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“…A total of 41 E. faecium isolates from the feces of different healthy students were used as control strains. The laboratory strains and plasmids used in this study were E. faecalis FA2-2 (Rif r , Fus r ) [17], JH2SS (Str r , Spc r ) [18], E. faecium BM4105RF (Rif r , Fus r ) [15], BM4105SS (Str r , Spc r ) [15], plasmid pMG1 (gentamicin resistances) (65.1 kbp) [14], pAD1 [19][20][21][22][23][24][25], pPD1 [26][27][28], pAM373 [29], pAMb1 [30], pIP501 [31,32] and pAM120 [33]. The media used in this study were Todd-Hewitt broth (THB) and agar plates (Difco Laboratories, Detroit, Mich.).…”

Section: Bacteria Plasmid and Mediamentioning

confidence: 99%

Drug resistance ofEnterococcus faeciumclinical isolates and the conjugative transfer of gentamicin and erythromycin resistance traits

Takeuchi

Tomita

Fujimoto

et al. 2005

FEMS Microbiology Letters

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Drug resistance and the transferability of resistance were examined in 218 Enterococcus faecium clinical isolates obtained from in-patients of a Japanese university hospital between 1990 and 1999. One hundred and sixty one isolates (73.9%) were drug-resistant and 127 (58.2%) isolates were resistant to two or more drugs. Vancomycin resistant E. faecium (VRE) was not isolated. The transferability of drug-resistance to an E. faecium strain was examined by broth or filter mating. Six (12.5%) of the 48 gentamicin resistance traits, and fifty (50%) of the 101 erythromycin resistance traits were transferred by filter mating. The gentamicin resistance traits of five isolates and the erythromycin resistance traits of four isolates were transferred to the recipient strains by both broth mating and filter mating at a frequency of about 10(-6) and 10(-5) per donor cell, respectively. The five gentamicin resistant strains were shown to harbor pMG1-like plasmids on the basis of their Southern hybridization with pMG1 (65.1 kbp, Gm(r)), which transfers efficiently between enterococci by broth mating. Each of the four erythromycin resistant transconjugants obtained by broth mating harbored a large conjugative plasmid (more than 100 kbp). The plasmids showed no homology with well-characterized enterococcal conjugative plasmids such as pAD1, pPD1, pAM(beta)1, pIP501 and pMG1 by Southern hybridization. Of the erythromycin resistance traits that transferred only by filter mating, it was found that the erythromycin resistance trait was conferred by a 47-kbp transposable element that transferred from the chromosome of the donor strain to different sites within the pheromone responsive plasmid pAD1 (60 kbp) of the recipient strain, suggesting that the erythromycin resistance trait was encoded on a conjugative transposon, which was named Tn950.

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Section: Bacteria Plasmid and Mediamentioning

confidence: 99%

Drug resistance ofEnterococcus faeciumclinical isolates and the conjugative transfer of gentamicin and erythromycin resistance traits

Takeuchi

Tomita

Fujimoto

et al. 2005

FEMS Microbiology Letters

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“…The most frequently used shuttle vectors in strain ATCC 824 are derived from either Enterococcus faecalis pAM␤1 (Leblanc and Lee, 1984), C. butyricum pCBU2 (Luczak et al, 1985), or Bacillus subtilis pIM13 (Monod et al, 1986). Both replicative and non-replicative plasmids (used for insertional gene inactivation) carry genes specifying resistance to erythromycin.…”

Section: Introductionmentioning

confidence: 99%

Genetic manipulation of acid and solvent formation in Clostridium acetobutylicum ATCC 824

Green

Bennett

1998

Biotechnol. Bioeng.

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The genes coding for enzymes involved in butanol or butyrate formation were subcloned into a novel Escherichia coli-Clostridium acetobutylicum shuttle vector constructed from pIMP1 and a chloramphenicol acetyl transferase gene. The resulting replicative plasmids, referred to as pTHAAD (aldehyde/alcohol dehydrogenase) and pTHBUT (butyrate operon), were used to complement C. acetobutylicum mutant strains, in which genes encoding aldehyde/alcohol dehydrogenase (aad) or butyrate kinase (buk) had been inactivated by recombination with Em r constructs. Complementation of strain PJC4BK (buk mutant) with pTHBUT restored butyrate kinase activity and butyrate production during exponential growth. Complementation of strain PJC4AAD (aad mutant) with pTHAAD restored NAD(H)-dependent butanol dehydrogenase activity, NAD(H)-dependent butyraldehyde dehydrogenase activity and butanol production during solventogenic growth. The development of an alternative selectable marker makes it is possible to overexpress genes, via replicative plasmids, in mutant strains that lack specific enzyme activities, thereby expanding the number of possible genetic manipulations that can be performed in C. acetobutylicum.

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“…The additional 2.OX1O6 fragment may thus originate from an erm gene insert of about O.8X1O6 on the 1.3X106 Hind111 fragment. A more detailed comparison of the HindIII-generated restriction fragment patterns for plasmids pSE703, pSE702 and pAMPl (27) revealed two common fragments, of 2.37X lo6 and 2.1 X lo6, respectively, in all three: one of 2.03X lo6 in pSE702 and pAMPl but not in pSE703, and one of 1.3X lo6 in pSE703 which was not present in pSE702. This led to the assumption that Fig.…”

Section: Restriction Enzyme Analysis and Hybridizationmentioning

confidence: 97%

Characterization of an erythromycin resistance (erm) plasmid in Streptococcus pyogenes

Schalén

Gebreselassie

Ståhl

1995

APMIS

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Three erythromyxin‐resistant Swedish isolates of Streptococcus pyogenes, representing different T‐types, were studied. Two of the strains showed constitutive high‐level (MIC>200 μg/ml) and one showed moderate (MIC 6.4 μg/ml) resistance; the latter strain was sensitive to lincosamide and clindamycin, and resistance was not induced by erythromycin. In each of the strains, a plasmid with an estimated Mw of 17.6±0.9×106 was isolated in addition to smaller cryptic plasmids. The three plasmids pSE701, pSE702 and pSE703 had very similar restriction enzyme cleavage patterns. Novobiocin curing of the high‐level resistance strain ER559 showed the resistance to be linked to its 17.6×106 plasmid, pSE703. Furthermore, by electroporation this rather large plasmid was reintroduced into an erythromycin‐sensitive cured derivative, acquiring resistance, and the plasmid was again recovered from the transconjugant. One of the plasmids, pSE702, was shown by filter mating to be conjugative within S. pyogenes. DNA‐DNA hybridization showed that the resistance determinant of the present three isolates was related to the erm gene on plasmid pAMpl of Enterococcus faecalis but not to that of plasmid pE194 of Staphylococcus aureus. The copy numbers of pSE702 and pSE703, derived from the two high‐level resistant strains, were 11±3 and 17±5 compared to 2±1 for pSE701, derived from the moderately resistant strain, possibly accounting for the phenotypic variation observed. The plasmids pSE702 and pAMpM showed about 80% homology in DNA‐DNA hybridization tests and high similarity in their restriction maps.

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Physical and genetic analyses of streptococcal plasmid pAM beta 1 and cloning of its replication region

Cited by 104 publications

References 34 publications

Drug resistance ofEnterococcus faeciumclinical isolates and the conjugative transfer of gentamicin and erythromycin resistance traits

Drug resistance ofEnterococcus faeciumclinical isolates and the conjugative transfer of gentamicin and erythromycin resistance traits

Genetic manipulation of acid and solvent formation in Clostridium acetobutylicum ATCC 824

Characterization of an erythromycin resistance (erm) plasmid in Streptococcus pyogenes

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