Physical and genetic mapping of barley (
            <i>Hordeum vulgare</i>
            ) germin-like cDNAs

Druka, Arnis; Kudrna, David; Kannangara, C. Gamini; Wettstein, Diter von; Kleinhofs, A.

doi:10.1073/pnas.022627999

Cited by 58 publications

(58 citation statements)

References 39 publications

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“…Sequencing of this 680-bp cDNA revealed 100 and 99% nucleotide identity with the barley GerB and GerF genes, respectively. These two paralogs are part of a gene family in barley that encodes GLPs (Wu et al 2000;Druka et al 2002). Southern blot analysis of Morex genomic DNA, hybridized with the 680-bp GerB cDNA, confirmed that GerB belongs to a relatively small gene family ( Figure 1A).…”

Section: Resultsmentioning

confidence: 87%

“…Confounding effects of gene conversion events cannot be dismissed since GerB and GerF are two closely linked loci (Druka et al 2002). However, high protein homogeneity has been maintained in this barley gene family; K a /K s ratios between unlinked gene family members are ,0.3 for all pairs tested (supplemental Table 1 at http://www.genetics.org/supplemental/), which strongly indicates high function constraint of protein evolution.…”

Section: Resultsmentioning

confidence: 99%

“…Also, sequence analysis of barley EST libraries did not strictly discriminate the expression patterns of these two genes, due to their highly conserved nature (Druka et al 2002). In this report, the use of single-stranded conformation polymorphism (SSCP) analysis of RT-PCR products allowed us to elucidate the developmental and tissue-specific expression patterns of each gene, providing evidence of both overlapping redundancy and early subfunctionalization.…”

mentioning

confidence: 99%

“…GerB and GerF are two closely linked loci on barley chromosome 1 (7H) bin 8 and belong to the HvGER-III subfamily (Druka et al 2002). Both genes share high nucleotide sequence identity in coding and noncoding regions.…”

mentioning

confidence: 99%

“…One hundred twenty-four germin and germin-like barley cDNAs have been grouped according to their amino acid homology into five subfamilies, designated HvGER-I to HvGER-V (Druka et al 2002). GerB and GerF are two closely linked loci on barley chromosome 1 (7H) bin 8 and belong to the HvGER-III subfamily (Druka et al 2002).…”

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confidence: 99%

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Spatial and Temporal Divergence of Expression in Duplicated Barley Germin-Like Protein-Encoding Genes

et al. 2006

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Subfunctionalization is the process by which a pair of duplicated genes, or paralogs, experiences a reduction of individual expression patterns or function while still reproducing the complete expression pattern and function of the ancestral gene. Two germin-like protein (GLP)-encoding genes, GerB and GerF, are paralogs that belong to a small gene family in barley (Hordeum vulgare). Both genes share high nucleotide sequence similarity in coding and noncoding regions and encode identical apoplastic proteins. The use of RNA gel blots, coupled with single-stranded conformation polymorphism (SSCP) analysis of RT–PCR products, elucidated the developmental and tissue-specific expression patterns of each gene. Individual expression patterns provided evidence of both overlapping redundancy and early subfunctionalization. GerB is predominantly expressed in developing shoots, while GerF is predominantly expressed in seedling roots, developing spikes, and pericarp/testa. GerF promoter deletion studies located a region (−356/−97) responsible for high promoter activity and showed the ability of GerB and GerF upstream regions to drive gfp expression in coleoptiles, epicarps, and lemma/palea of developing spikes. The observed expression patterns are consistent with proposed roles in plant development and defense mechanisms for this gene family. These roles may explain why redundancy has been selectively maintained in this duplicate gene pair.

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Section: Resultsmentioning

confidence: 87%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Spatial and Temporal Divergence of Expression in Duplicated Barley Germin-Like Protein-Encoding Genes

et al. 2006

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A rapid identification of barley varieties using DNA-AFLP

Xue

Chu

2015

J. Inst. Brew.

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Barley (Hordeum vulgare L.) is one of the most ancient crops in the world, and is mainly used in feed, as a grain and as industrial material for beer, medicine and health protection. Its purity can affect beer taste and quality, thus it is important to set up rapid and accurate identification methods for the malting industry. In this study, 27 domestic and imported malt barley varieties were studied. Three pair primers of EcoR I + 3/Mse I + 3 were selected from 64 different primer combinations for DNA–AFLP (amplified fragment length polymorphisms) and an AFLP fingerprinting map of the 27 domestic and imported malt barley varieties was constructed, with the primer combination of E‐AGG/M‐CAA amplification after polyacrylamide gel electrophoresis. With this fingerprinting map, it was possible to distinguish all of the 27 malt barley varieties with 10 specific loci. This could be useful for the identification of malt barley varieties in the beer industry. Copyright © 2015 The Institute of Brewing & Distilling

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Molecular Maps in Cereals: Methodology and Progress

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Physical and genetic mapping of barley ( Hordeum vulgare ) germin-like cDNAs

Cited by 58 publications

References 39 publications

Spatial and Temporal Divergence of Expression in Duplicated Barley Germin-Like Protein-Encoding Genes

Spatial and Temporal Divergence of Expression in Duplicated Barley Germin-Like Protein-Encoding Genes

A rapid identification of barley varieties using DNA-AFLP

Molecular Maps in Cereals: Methodology and Progress

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