Physical and genetic mapping of the genomes of five Mycoplasma hominis strains by pulsed-field gel electrophoresis

Ladefoged, Søren; Christiansen, Gunna

doi:10.1128/jb.174.7.2199-2207.1992

Cited by 99 publications

(97 citation statements)

References 38 publications

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“…(ii) The second method was similar to that of Ladefoged et al [8]. Chromosomal DNA was completely cleaved with the followmg restriction enzymes; AccI, BarnHI.…”

Section: Methodsmentioning

confidence: 99%

Physical map of the extremely thermophilic bacterium Thermus thermophilus HB27 chromosome

et al. 1993

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“…(ii) The second method was similar to that of Ladefoged et al [8]. Chromosomal DNA was completely cleaved with the followmg restriction enzymes; AccI, BarnHI.…”

Section: Methodsmentioning

confidence: 99%

Physical map of the extremely thermophilic bacterium Thermus thermophilus HB27 chromosome

et al. 1993

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“…horninis PG21. However, DNA probes from the gene (termed p120) encoding P120 hybridized to DNA from all 19 M. horninis strains including PG21, and on the chromosomal maps of five M. horninis strains (PG21,4195,132,93 and 7488) the pl20 gene was located at similar positions close to the gyrB gene (Ladefoged & Christiansen, 1992). To study whether this variable, surface-localized protein was recognized by the human humoral immune response we studied this system in detail.…”

Section: Introductionmentioning

confidence: 99%

The Mycoplasma hominis P120 membrane protein contains a 216 amino acid hypervariable domain that is recognized by the human humoral immune response

1997

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The epitope for mAb 26.7D was mapped to the hypervariable domain by expression of various parts of this domain in Escherichia coli using expression vector systems. A polyclonal antiserum (pAb 121) generated against the hypervariable region of Pl20 from PG2l identified the Pl20 homologue in M. hominis PG21. Fusion proteins of the hypervariable and constant parts of the proteins were constructed and tested for reactivity with 21 human sera. Twelve sera reacted with the 7488 hypervariable fusion protein, but only four reacted with the PG2l hypervariable fusion protein. No reactivity was seen with a fusion protein containing part of the constant region of P120. Gene fragments amplified from 18 M. hominis isolates by PCR confirmed the heterogeneity of the hypervariable domain. Based on restriction endonuclease cleavage patterns of the hypervariable domain the 18 isolates could be divided into four classes. Reactivity with both mAb 26.71) and pAb 121 confirmed these classes. The hypervariable, but not the constant, part of PI20 was recognized by the human humoral immune response. Such a variable domain may be important in evasion of the host's immune response, and thus aid survival of the micro-organism.

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“…This can be explained by the fact that these sites were mainly located in the vicinity of the rrn operons which are known to have a higher G+C content -47 mol% in the case of the FD-P 16S rDNA, as is the case for other mollicute genomes (Barré et al, 2004;Razin et al, 1998;Sirand-Pugnet et al, 2007). The concentration of G+C-rich restriction sites in the vicinity of the rrn operons has also been observed on physical maps of Mycoplasma hominis (Ladefoged & Christiansen, 1992) and Spiroplasma citri strains (Ye et al, 1992). 51/37 600 bp, i.e.…”

Section: Discussionmentioning

confidence: 88%

“…In addition, the Mycoplasma mycoides subsp. mycoides Y and M. hominis physical maps showed frequencies of restriction sites containing one A and one T (BamHI, KpnI, SalI and XhoI) six to ten times lower than the theoretical ones (Ladefoged & Christiansen, 1992;Pyle & Finch, 1988). This phenomenon could be due to methylation of the majority of these restriction sites.…”

Section: Discussionmentioning

confidence: 93%

A chromosome map of the Flavescence dorée phytoplasma

et al. 2008

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The Flavescence doré e phytoplasma (FD-P), a non-cultivable, plant-pathogenic bacterium of the class Mollicutes, is the causal agent of a quarantine disease affecting vineyards of southern Europe, mainly in southern France and northern Italy. To investigate FD-P diversity and phytoplasma genetic determinants governing the FD-P life cycle, a genome project has been initiated. A physical map of the chromosome of FD-P strain FD92, purified from infected broad beans, was constructed by performing restriction digests of the chromosome and resolving the fragments by PFGE. Single and double digestions of the chromosome with the enzymes SalI, BssHII, MluI and EagI were performed and used to map 13 restriction sites on the FD-P chromosome. The size of the chromosome was calculated to be 671 kbp. Southern blot analyses using cloned phytoplasma probes were carried out to assist in the arrangement of contiguous restriction fragments and to map eight genetic loci, including the two rRNA operons, the tuf, uvrB-degV and secY-map (FD9) genes, the FD2 marker and two orphan sequences (FDDH29 and FDSH05) isolated through subtractive suppression hybridization.

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Physical and genetic mapping of the genomes of five Mycoplasma hominis strains by pulsed-field gel electrophoresis

Cited by 99 publications

References 38 publications

Physical map of the extremely thermophilic bacterium Thermus thermophilus HB27 chromosome

Physical map of the extremely thermophilic bacterium Thermus thermophilus HB27 chromosome

The Mycoplasma hominis P120 membrane protein contains a 216 amino acid hypervariable domain that is recognized by the human humoral immune response

A chromosome map of the Flavescence dorée phytoplasma

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