Physical and genetic studies of Newcastle disease virus: Evidence for multiploid particles

Dahlberg, John E.; Simon, Edward H.

doi:10.1016/0042-6822(69)90185-8

Cited by 46 publications

(21 citation statements)

References 18 publications

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“…The major peak indicated R264, whereas the minor one indicated the wt G264 (overlooked in our initial examination), suggesting that the P3-2 virus contained two types of genomes differing in the F gene. As paramyxoviruses are known to have the ability to include several genomes in one viral particle, called polyploidy [12][13][14] , our observation is not unexpected.…”

Section: Fusion Assay Inconsistent With One Recombinant Virussupporting

confidence: 54%

Cooperation between different RNA virus genomes produces a new phenotype

2012

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Section: Fusion Assay Inconsistent With One Recombinant Virussupporting

confidence: 54%

Cooperation between different RNA virus genomes produces a new phenotype

2012

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“…Paramyxoviruses encode their genomes on a single strand of negative-sense RNA. Evidence for multiploid paramyxoviruses, including NDV, emerged in the mid-20th Century (4). Notably, the paramyxoviral ribonucleoproteins (vRNPs) are characteristically disordered, in contrast to some other negative-sense RNA viruses, like influenza A virus with its distinctive "7 ϩ 1" RNP arrangement, and more research is needed to refine our understanding of paramyxovirus genomic content (6,8,10,14).…”

mentioning

confidence: 99%

A Majority of Infectious Newcastle Disease Virus Particles Contain a Single Genome, while a Minority Contain Multiple Genomes

Goff

Gao

Palese

2012

J Virol

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Paramyxoviruses produce pleiomorphic particles containing variable amounts of genetic material that correlate with virion diameter by electron microscopy. However, the infectious nature of these particles is unknown, and functional genomes are indistinguishable from defective RNA. A quantitative approach to paramyxovirus packaging revealed a majority of infectious Newcastle disease viruses contain one functional genome. Virions encapsidating two or three genomes (approximately 25% of total) were also observed by utilizing three different recombinant viruses expressing unique fluorescent reporters.

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“…Previous studies showed that some paramyxoviruses in the order Mononegavirales produce significant amounts of polyploid particles (4,7,11,22). Rager et al and others have suggested that segmented RNA genomes may have evolved from such nonsegmented genomes incorporated in the polyploid particles by the reduction of redundant genetic regions (4,7,11,22).…”

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confidence: 99%

Generation of Measles Virus with a Segmented RNA Genome

Takeda

Nakatsu

Ohno

et al. 2006

J Virol

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Viruses classified in the order Mononegavirales have a single nonsegmented RNA molecule as the genome and employ similar strategies for genome replication and gene expression. Infectious particles of Measles virus (MeV), a member of the family Paramyxoviridae in the order Mononegavirales, with two or three RNA genome segments (2 seg-or 3 seg-MeV) were generated using a highly efficient reverse genetics system. All RNA segments of the viruses were designed to have authentic 3 and 5 self-complementary termini, similar to those of negative-stranded RNA viruses that intrinsically have multiple RNA genome segments. The 2 seg-and 3 seg-MeV were viable and replicated well in cultured cells. 3 seg-MeV could accommodate up to six additional transcriptional units, five of which were shown to be capable of expressing foreign proteins efficiently. These data indicate that the MeV genome can be segmented, providing an experimental insight into the divergence of the negative-stranded RNA viruses with nonsegmented or segmented RNA genomes. They also illustrate a new strategy to develop mononegavirus-derived vectors harboring multiple additional transcriptional units.

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Physical and genetic studies of Newcastle disease virus: Evidence for multiploid particles

Cited by 46 publications

References 18 publications

Cooperation between different RNA virus genomes produces a new phenotype

Cooperation between different RNA virus genomes produces a new phenotype

A Majority of Infectious Newcastle Disease Virus Particles Contain a Single Genome, while a Minority Contain Multiple Genomes

Generation of Measles Virus with a Segmented RNA Genome

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