Physical approaches for drug delivery

Shinde, Pallavi; Kumar, Amogh; Kavitha,; Dey, Koyel; Mohan, L.; Kar, Siddhartha; Barik, Tarun Kumar; Sharifi‐Rad, Javad; Nagai, Masao; Santra, Tuhin Subhra

doi:10.1016/b978-0-12-817776-1.00007-9

Cited by 27 publications

(14 citation statements)

References 42 publications

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“…Performance, efficiency, and sustainability of industrial processes that involve multiphase flows are determined by the way different phases are contacted. Examples can be found in (bio) chemical engineering, , medicine, , food production processes, , mining, , and water and wastewater treatment , among others. , There, very often the gas phase is dispersed into the continuous phase in the form of bubbles. Reducing the bubble size at the same gas flow increases mass transfer.…”

Section: Introductionmentioning

confidence: 99%

A Mechanistic Model for Bubble Formation from Microscale Orifices under Constant Gas Flow Conditions

et al. 2021

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We developed a mechanistic model for calculation of bubble volume from orifices in the range from 0.03 mm to 0.193 mm under the constant gas flow conditions in a quiescent liquid. It is known that for such small orifices, the mechanism of bubble formation is highly dependent on the gas momentum force and the liquid inertia force. Accordingly, the model incorporates these forces to calculate the bubble volume in three consecutive stages. Moreover, the model includes the influence of the bubble base expansion and bubble rising induced liquid velocity on the formation of bubbles. Eventually the model is validated with our own experimental data using air and deionized water. Experimental validation of the model confirms that the maximum deviation of the model is less than 10%.

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Section: Introductionmentioning

confidence: 99%

A Mechanistic Model for Bubble Formation from Microscale Orifices under Constant Gas Flow Conditions

et al. 2021

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“…Genes delivered via cationic polymers may be targeted to endolysosomes and result in endocytic degradation [114]. On the other hand, physical transfection methods use physical energy to create temporary pores on the cell membrane that allow foreign biomolecules into the cells by simple diffusion [115][116][117]. In the last two decades, due to the rapid development of micro-and nano-technologies, many physical techniques can deliver different sized biomolecules in different cell types (at a single-cell level) with high transfection efficiency and high cell viability [3,6,7].…”

Section: Single-neuron Transfection Methodsmentioning

confidence: 99%

A Single-Neuron: Current Trends and Future Prospects

Gupta

Balasubramaniam

Chang

et al. 2020

Cells

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The brain is an intricate network with complex organizational principles facilitating a concerted communication between single-neurons, distinct neuron populations, and remote brain areas. The communication, technically referred to as connectivity, between single-neurons, is the center of many investigations aimed at elucidating pathophysiology, anatomical differences, and structural and functional features. In comparison with bulk analysis, single-neuron analysis can provide precise information about neurons or even sub-neuron level electrophysiology, anatomical differences, pathophysiology, structural and functional features, in addition to their communications with other neurons, and can promote essential information to understand the brain and its activity. This review highlights various single-neuron models and their behaviors, followed by different analysis methods. Again, to elucidate cellular dynamics in terms of electrophysiology at the single-neuron level, we emphasize in detail the role of single-neuron mapping and electrophysiological recording. We also elaborate on the recent development of single-neuron isolation, manipulation, and therapeutic progress using advanced micro/nanofluidic devices, as well as microinjection, electroporation, microelectrode array, optical transfection, optogenetic techniques. Further, the development in the field of artificial intelligence in relation to single-neurons is highlighted. The review concludes with between limitations and future prospects of single-neuron analyses.

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“…Considering these limitations, physical approaches for drug delivery have been developed over the years. − One of the most promising physical methods is electroporation; this is an easy-to-use technique, which enables gene transfer in different cell types within a short process time with controllable pore size and reduced cytosolic leakage. − However, it involves pH variation, electric field distortion, and electrolysis effect that reduce the cell viability. ,,, Microinjection technique uses a micropipette to inject biomolecules into the living cell, but it is time-consuming, limited by low-throughput delivery, and requires a skilled person for operation. The sonoporation technique generates acoustic pressure to disrupt the plasma membrane for molecular delivery.…”

Section: Introductionmentioning

confidence: 99%

Infrared Pulse Laser-Activated Highly Efficient Intracellular Delivery Using Titanium Microdish Device

Shinde

Kar

Mohan

et al. 2020

ACS Biomater. Sci. Eng.

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We report infrared (IR) pulse laser-activated highly efficient parallel intracellular delivery by using an array of titanium microdish (TMD) device. Upon IR laser pulse irradiation, a two-dimensional array of TMD device generated photothermal cavitation bubbles to disrupt the cell membrane surface and create transient membrane pores to deliver biomolecules into cells by a simple diffusion process. We successfully delivered the dyes and different sizes of dextran in different cell types with variations of laser pulses. Our platform has the ability to transfect more than a million cells in a parallel fashion within a minute. The best results were achieved for SiHa cells with a delivery efficiency of 96% and a cell viability of around 98% for propidium iodide dye using 600 pulses, whereas a delivery efficiency of 98% and a cell viability of 100% were obtained for dextran 3000 MW delivery using 700 pulses. For dextran 10,000 MW, the delivery efficiency was 92% and the cell viability was 98%, respectively. The device is compact, easy-to-use, and potentially applicable for cellular therapy and diagnostic purposes.

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Physical approaches for drug delivery

Cited by 27 publications

References 42 publications

A Mechanistic Model for Bubble Formation from Microscale Orifices under Constant Gas Flow Conditions

A Mechanistic Model for Bubble Formation from Microscale Orifices under Constant Gas Flow Conditions

A Single-Neuron: Current Trends and Future Prospects

Infrared Pulse Laser-Activated Highly Efficient Intracellular Delivery Using Titanium Microdish Device

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