Physical basis of some membrane shaping mechanisms

Šimunović, Mijo; Prévost, Coline; Callan-Jones, Andrew; Bassereau, Patricia

doi:10.1098/rsta.2016.0034

Cited by 43 publications

(39 citation statements)

References 97 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ability of proteins to generate membrane curvature has been extensively explored both experimentally and theoretically over the past decade (for recent reviews, see Jarsch et al, 2016;McMahon and Boucrot, 2015;Simunovic et al, 2016). Less emphasis has been put on the isotropic versus anisotropic character of the local membrane shape created by each of the specific curvature-generating proteins.…”

Section: Membrane Curvature Generationmentioning

confidence: 99%

Membrane remodeling in clathrin-mediated endocytosis

Haucke

Kozlov

2018

Journal of Cell Science

109

View full text Add to dashboard Cite

Clathrin-mediated endocytosis is an essential cellular mechanism by which all eukaryotic cells regulate their plasma membrane composition to control processes ranging from cell signaling to adhesion, migration and morphogenesis. The formation of endocytic vesicles and tubules involves extensive protein-mediated remodeling of the plasma membrane that is organized in space and time by protein-protein and protein-phospholipid interactions. Recent studies combining high-resolution imaging with genetic manipulations of the endocytic machinery and with theoretical approaches have led to novel multifaceted phenomenological data of the temporal and spatial organization of the endocytic reaction. This gave rise to various - often conflicting - models as to how endocytic proteins and their association with lipids regulate the endocytic protein choreography to reshape the plasma membrane. In this Review, we discuss these findings in light of the hypothesis that endocytic membrane remodeling may be determined by an interplay between protein-protein interactions, the ability of proteins to generate and sense membrane curvature, and the ability of lipids to stabilize and reinforce the generated membrane shape through adopting their lateral distribution to the local membrane curvature.

show abstract

Section: Membrane Curvature Generationmentioning

confidence: 99%

Membrane remodeling in clathrin-mediated endocytosis

Haucke

Kozlov

2018

Journal of Cell Science

109

View full text Add to dashboard Cite

show abstract

“…The capability to measure or control membrane tension by micropipette aspiration is also useful when combined with other methods. For instance, tube or tether pulling, either with molecular motors, micropipiette, or an optical trap can be used to study curvature sensitive processes such as lipid sorting or protein binding . However, a drawback of micropipette aspiration experiments is the heterogeneity of applied membrane tension due to contact with the pipette tip.…”

Section: Experimental Systems For the Study Of Lipid Bilayersmentioning

confidence: 99%

Systems biology of cellular membranes: a convergence with biophysics

Chabanon

Stachowiak

Rangamani

2017

WIREs Mechanisms of Disease

View full text Add to dashboard Cite

Systems biology and systems medicine have played an important role in the last two decades in shaping our understanding of biological processes. While systems biology is synonymous with network maps and ‘-omics’ approaches, it is not often associated with mechanical processes. Here, we make the case for considering the mechanical and geometrical aspects of biological membranes as a key step in pushing the frontiers of systems biology of cellular membranes forward. We begin by introducing the basic components of cellular membranes, and highlight their dynamical aspects. We then survey the functions of the plasma membrane and the endomembrane system in signaling, and discuss the role and origin of membrane curvature in these diverse cellular processes. We further give an overview of the experimental and modeling approaches to study membrane phenomena. We close with a perspective on the converging futures of systems biology and membrane biophysics, invoking the need to include physical variables such as location and geometry in the study of cellular membranes.

show abstract

“…Here we show that the dynamics of reshaping conforms a very rich process with many intermediate steps, including phase separation between isotropic and nematic phases, and with major reshaping processes occurring at low coverage and curvature. The curvature sensing and membrane reshaping properties of BAR proteins have been extensively studied on highly curved tubes (up to 100 nm in diameter), mostly in equilibrium 4,29,30,31 . However, many cell studies pointed out the role of BAR proteins acting on lower curvature lipid structures 32 .…”

Section: Mainmentioning

confidence: 99%

Dynamic Mechanochemical feedback between curved membranes and BAR protein self-organization

Roux

Tozzi

Walani

et al. 2020

Preprint

View full text Add to dashboard Cite

In many physiological situations, BAR proteins interact with, and reshape, pre-existing curved membranes, contributing to essential cellular processes. However, the non-equilibrium and timedependent process of reshaping, and its dependence on initial membrane shape, remains largely unknown. Here we explain, both experimentally and through modelling, how a BAR protein dynamically interacts with mechanically bent lipid membranes. We capture protein binding to curved membranes, and characterize a variety of dynamical reshaping events depending on membrane shape and protein arrangement. The events can be generally understood by an isotropic-to-nematic phase transition, in which low curvature templates with isotropic protein orientation progress towards highly curved lipid tubes with nematic protein arrangement. Our findings also apply in cells, where mechanical stretch triggers BAR-protein-membrane interactions that enable potential mechanotransduction mechanisms. Our results characterize and broaden the reshaping processes of BAR proteins on mechanically constrained membranes, demonstrating the interplay between membrane mechanical stimuli and BAR protein response.

show abstract

Physical basis of some membrane shaping mechanisms

Cited by 43 publications

References 97 publications

Membrane remodeling in clathrin-mediated endocytosis

Membrane remodeling in clathrin-mediated endocytosis

Systems biology of cellular membranes: a convergence with biophysics

Dynamic Mechanochemical feedback between curved membranes and BAR protein self-organization

Contact Info

Product

Resources

About