Physical characterization of hydroxyapatite porous scaffolds for tissue engineering

Teixeira, Sérgio; Rodrı́guez, Miguel Á.; Pena, P.; Aza, Antonio H. De; Aza, S. De; Ferraz, Maria Pia; Monteiro, Fernando J.

doi:10.1016/j.msec.2008.09.052

Cited by 112 publications

(64 citation statements)

References 22 publications

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“…34 The samples were evaluated under compressive mechanical testing, with a maximum compressive stress value of 0.07 6 0.03 N/mm 2 . These values are in accordance with the literature.…”

Section: Scaffold Characterizationmentioning

confidence: 99%

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In vivo evaluation of highly macroporous ceramic scaffolds for bone tissue engineering

Teixeira

Fernandes

Leusink

et al. 2009

J Biomedical Materials Res

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During the last decades, different materials of both natural and synthetic origin have been developed with the aim of inducing and controlling osteogenic differentiation of mesenchymal stem cells (MSCs). In order for that to happen, it is necessary that the material to be implanted obey a series of requirements, namely: osteoconduction, biocompatibility, and biodegradability. Additionally, they must be low-priced, easy to produce, shape, and store. Hydroxyapatite (HA) is a well known ceramic with a composition similar to the mineral component of bone and is highly biocompatible and easy to obtain and/or process. On the other hand, collagen is the main structural protein present in the human body and bone. In this study, a polymer replication method was applied and a highly porous HA scaffold was produced. Collagen was later incorporated to improve the biological properties of the scaffold while resembling the bone composition. The scaffolds were characterized by means of scanning electron microscopy, Fourier transform infrared spectroscopy and energy dispersive spectroscopy. In vitro and in vivo testing was performed in all scaffolds produced. The goal of this study was to evaluate the in vivo osteogenic potential of MSCs from two different species seeded on the different HA basedporous scaffolds with collagen type I. The resultsindicate that all scaffolds exhibit relevant bone formation, being more prominent in the case of the HA scaffolds.

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“…34 The samples were evaluated under compressive mechanical testing, with a maximum compressive stress value of 0.07 6 0.03 N/mm 2 . These values are in accordance with the literature.…”

Section: Scaffold Characterizationmentioning

confidence: 99%

“…35 In addition, the results have indicated that the fracture is initiated at the scaffolds inner core and rupture was due to the reduced pore strut thickness. 34 To observe the presence of collagen on the HA scaffolds, energy dispersive spectroscopy (EDS) characterization was performed (Fig. 2).…”

Section: Scaffold Characterizationmentioning

confidence: 99%

In vivo evaluation of highly macroporous ceramic scaffolds for bone tissue engineering

Teixeira

Fernandes

Leusink

et al. 2009

J Biomedical Materials Res

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“…Therefore, a composite biomaterial of HA and chitosan is expected to show increased osteoconductivity and biodegradation together with sufficient mechanical strength for orthopedic use. In recent years, the interest in biomaterials such as hydroxyapatite and chitosan has increased significantly, evidenced by the significant growth in the number of scientific articles reporting their characterization and evaluation [8].…”

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confidence: 99%

Chitosan/hydroxyapatite scaffolds for tissue engineering manufacturing method effect comparison

Escobar-Sierra

Martins

Orozco

2015

Rev. Fac. Ing. Antioquia

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The regeneration of bone is one of the main challenges of modern medicine because many diseases, including trauma and tumor, can cause bone defects. Tissue engineering (TE) is a promising approach to cure these bone diseases because it allows the reconstruction of tissue by colonization and proliferation of healthy cells in an artificial extracellular matrix (scaffolds). The aim of this project was to prepare chitosan/hydroxyapatite CH/HA scaffolds, using various ratios and two different methods: powder hydroxyapatite (commercial) and in situ hydroxyapatite, and then compare their properties. The morphology, chemical composition and mechanical properties were evaluated by Scanning Electron Microscopy (SEM), X-ray diffraction (XRD), Raman spectroscopy, and compression tests. The scaffolds obtained showed an interconnected porous structure. The scaffolds with chitosan and hydroxyapatite manufacturing by in situ protocol, have better applications in tissue engineering, because they have a better morphology and allow the cell growth. These scaffolds are suitable for tissue engineering.----------Keywords: bone, chitosan, hydroxyapatite in situ, hydroxyapatite in powder, scaffolds chitosan/ hydroxyapatite, tissue engineering

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“…Os microporos observados contribuem diretamente para formação de interconectividade entre os poros [20]. A Figura 6 apresenta a distribuição diferencial do tamanho de poros dos scaffolds desenvolvidos.…”

Section: Espectroscopia No Infravermelho Com Transformada De Fourier unclassified

Desenvolvimento de biocerâmicas porosas de hidroxiapatita para utilização como scaffolds para regeneração óssea

Fook

Aparecida²,

Fook

2010

Matéria (Rio J.)

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Biocerâmicas porosas tem aplicações biomédicas importantes como preenchimento de defeitos ósseos e scaffolds na engenharia de tecidos. A hidroxiapatita (HA, Ca10(PO4)6(OH)2) que apresenta semelhança química e estrutural com a fase mineral dos ossos e dos dentes, é biocompatível e osteocondutiva, e tem excelente afinidade química e biológica com os tecidos ósseos. Este trabalho teve como objetivo desenvolver biocerâmicas porosas HA para utilização como scaffold para regeneração óssea empregando-se a técnica de réplica da esponja polimérica. A pasta biocerâmica de HA foi obtida por via úmida utilizando hidróxido de cálcio [Ca(OH)2] e ácido fosfórico (H3PO4) e impregnada em esponjas de poliuretano com diferentes densidades. Tratamento térmico a 600°C por 1h foi realizado para eliminação da esponja seguido da sinterização a 1100°C por 2 horas. Os scaffolds apresentaram a HA como fase majoritária, elevada porosidade (> 70%) e poros com tamanhos variando na ordem de macro (>100μm) e microporosidade (1-20μm), sendo estes fatores adequados para a aplicação como scaffolds para regeneração óssea.

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Physical characterization of hydroxyapatite porous scaffolds for tissue engineering

Cited by 112 publications

References 22 publications

In vivo evaluation of highly macroporous ceramic scaffolds for bone tissue engineering

In vivo evaluation of highly macroporous ceramic scaffolds for bone tissue engineering

Chitosan/hydroxyapatite scaffolds for tissue engineering manufacturing method effect comparison

Desenvolvimento de biocerâmicas porosas de hidroxiapatita para utilização como scaffolds para regeneração óssea

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