Physical, chemical, and functional properties of neuronal membranes vary between species of Antarctic notothenioids differing in thermal tolerance

Biederman, Amanda; Kuhn, Donald M.; O’Brien, Kristin M.; Crockett, Elizabeth L.

doi:10.1007/s00360-019-01207-x

Cited by 15 publications

(7 citation statements)

References 54 publications

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“…Here, we showed that the loss of synapsin, the specific protein maintaining synapse organization [28], was responsible for synapse impairment in the spinal cord. FM1-43 photobleaching tests showed that Eg5 inhibition abrogated fluorescence recovery, suggesting the roles of Eg5 in maintaining membrane [40]. Therefore, membrane fluidity perturbation induced by Eg5 inhibition contributed to synapse impairment.…”

Section: Discussionmentioning

confidence: 97%

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Inhibitions and Down-Regulation of Motor Protein Eg5 Expression in Primary Sensory Neurons Reveal a Novel Therapeutic Target for Pathological Pain

Wei

Yang

et al. 2022

Neurotherapeutics

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The motor protein Eg5, known as kif11 or kinesin-5, interacts with adjacent microtubules in the mitotic spindle and plays essential roles in cell division, yet the function of Eg5 in mature postmitotic neurons remains largely unknown. In this study, we investigated the contribution and molecular mechanism of Eg5 in pathological pain. Pharmacological inhibition of Eg5 and a specific shRNA-expressing viral vector reversed complete Freund’s adjuvant (CFA)-induced pain and abrogated vanilloid receptor subtype 1 (VR1) expression in dorsal root ganglion (DRG) neurons. In the dorsal root, Eg5 inhibition promoted VR1 axonal transport and decreased VR1 expression. In the spinal cord, Eg5 inhibition suppressed VR1 expression in axon terminals and impaired synapse formation in superficial laminae I/II. Finally, we showed that Eg5 is necessary for PI3K/Akt signalling-mediated VR1 membrane trafficking and pathological pain. The present study provides compelling evidence of a noncanonical function of Eg5 in primary sensory neurons. These results suggest that Eg5 may be a potential therapeutic target for intractable pain.

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Section: Discussionmentioning

confidence: 97%

“…FM1-43 photobleaching tests showed that Eg5 inhibition abrogated fluorescence recovery, suggesting the roles of Eg5 in maintaining membrane fluidity. Membrane fluidity contributes to the formation and function of synapses [ 40 ]. Therefore, membrane fluidity perturbation induced by Eg5 inhibition contributed to synapse impairment.…”

Section: Introductionmentioning

confidence: 99%

Inhibitions and Down-Regulation of Motor Protein Eg5 Expression in Primary Sensory Neurons Reveal a Novel Therapeutic Target for Pathological Pain

Wei

Yang

et al. 2022

Neurotherapeutics

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“…Each animal was used in a single behavioral experiment without repetition. Once animals reached endpoint criteria, they were promptly removed from the tank and transferred to other participants of the project for euthanasia and collection of tissues for biochemical assays [ 31 , 32 ]. The amount of time elapsed from the onset of LOE before euthanasia was always less than three minutes.…”

Section: Methodsmentioning

confidence: 99%

“…coriiceps were fed fish muscle blocks once every 2–3 days. Icefish do not feed in captivity [ 19 , 31 , 32 ], and these animals were used within two weeks of capture. A part of the legacy protocol, this was based on prior experience and on the notion that metabolic rate of icefishes is lower than other notothenioids and that they are thought to feed at relatively broad time intervals.…”

Section: Methodsmentioning

confidence: 99%

Antarctic teleosts with and without hemoglobin behaviorally mitigate deleterious effects of acute environmental warming

et al. 2021

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Recent studies forecast that many ectothermic animals, especially aquatic stenotherms, may not be able to thrive or even survive predicted climate change. These projections, however, generally do not call much attention to the role of behavior, an essential thermoregulatory mechanism of many ectotherms. Here we characterize species-specific locomotor and respiratory responses to acute ambient warming in two highly stenothermic Antarctic Notothenioid fishes, one of which (Chaenocephalus aceratus) lacks hemoglobin and appears to be less tolerant to thermal stress as compared to the other (Notothenia coriiceps), which expresses hemoglobin. At the onset of ambient warming, both species perform distinct locomotor maneuvers that appear to include avoidance reactions. In response to unavoidable progressive hyperthermia, fishes demonstrate a range of species-specific maneuvers, all of which appear to provide some mitigation of the deleterious effects of obligatory thermoconformation and to compensate for increasing metabolic demand by enhancing the efficacy of branchial respiration. As temperature continues to rise, Chaenocephalus aceratus supplements these behaviors with intensive pectoral fin fanning which may facilitate cutaneous respiration through its scaleless integument, and Notothenia coriiceps manifests respiratory-locomotor coupling during repetitive startle-like maneuvers which may further augment gill ventilation. The latter behaviors, found only in Notothenia coriiceps, have highly stereotyped appearance resembling Fixed Action Pattern sequences. Altogether, this behavioral flexibility could contribute to the reduction of the detrimental effects of acute thermal stress within a limited thermal range. In an ecologically relevant setting, this may enable efficient thermoregulation of fishes by habitat selection, thus facilitating their resilience in persistent environmental change.

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“…The answer may lie in the large dimensional changes the heart must endure with each heartbeat, because their cardiac stroke volume is routinely 8 times larger and maximum _ Q is up to 2.4 times larger than that of redblooded species (Egginton et al, 2019). However, higher membrane fluidity in icefishes is also observed in synaptic membranes from the brain (Biederman et al, 2019b). Thus, although enhanced fluidity may represent a liability in the face of a warming climate, it may also confer physiological benefits (i.e.…”

Section: Do Biophysical Properties Of Biological Membranes Underpin Thermal Tolerance?mentioning

confidence: 99%

Resilience of cardiac performance in Antarctic notothenioid fishes in a warming climate

O’Brien

Joyce

Crockett

et al. 2021

Journal of Experimental Biology

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Warming in the region of the Western Antarctic Peninsula is occurring at an unprecedented rate, which may threaten the survival of Antarctic notothenioid fishes. Herein, we review studies characterizing thermal tolerance and cardiac performance in notothenioids – a group that includes both red-blooded species and the white-blooded, haemoglobinless icefishes – as well as the relevant biochemistry associated with cardiac failure during an acute temperature ramp. Because icefishes do not feed in captivity, making long-term acclimation studies unfeasible, we focus only on the responses of red-blooded notothenioids to warm acclimation. With acute warming, hearts of the white-blooded icefish Chaenocephalus aceratus display persistent arrhythmia at a lower temperature (8°C) compared with those of the red-blooded Notothenia coriiceps (14°C). When compared with the icefish, the enhanced cardiac performance of N. coriiceps during warming is associated with greater aerobic capacity, higher ATP levels, less oxidative damage and enhanced membrane integrity. Cardiac performance can be improved in N. coriiceps with warm acclimation to 5°C for 6–9 weeks, accompanied by an increase in the temperature at which cardiac failure occurs. Also, both cardiac mitochondrial and microsomal membranes are remodelled in response to warm acclimation in N. coriiceps, displaying homeoviscous adaptation. Overall, cardiac performance in N. coriiceps is malleable and resilient to warming, yet thermal tolerance and plasticity vary among different species of notothenioid fishes; disruptions to the Antarctic ecosystem driven by climate warming and other anthropogenic activities endanger the survival of notothenioids, warranting greater protection afforded by an expansion of marine protected areas.

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Physical, chemical, and functional properties of neuronal membranes vary between species of Antarctic notothenioids differing in thermal tolerance

Cited by 15 publications

References 54 publications

Inhibitions and Down-Regulation of Motor Protein Eg5 Expression in Primary Sensory Neurons Reveal a Novel Therapeutic Target for Pathological Pain

Inhibitions and Down-Regulation of Motor Protein Eg5 Expression in Primary Sensory Neurons Reveal a Novel Therapeutic Target for Pathological Pain

Antarctic teleosts with and without hemoglobin behaviorally mitigate deleterious effects of acute environmental warming

Resilience of cardiac performance in Antarctic notothenioid fishes in a warming climate

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