Physical, Chemical, Molecular, and Immunological Methods

Influence of an enrichment step on Salmonella sp. detection by fluorescent in situ hybridization on pork samples

“…Some authors (Jay, 2000;Olsen et al, 1995) considered that the direct detection of food borne pathogens in food samples, without a previous pre-enrichment step, is a difficult task, due to its presence in a low number. However, slaughtered pork samples probably have enough bacteria to be directly detected trough FISH, since the results of this study revealed a high number of positive samples both over fresh and pre-enriched samples that were not significantly different.…”