Physical compensation of phase curvature in digital holographic microscopy by use of programmable liquid lens

Doblas, Ana; Hincapie, Diego; Saavedra, Genaro; Martı́nez-Corral, Manuel; Garcı́a-Sucerquia, Jorge

doi:10.1364/ao.54.005229

Cited by 30 publications

(4 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Single-shot physical methods have been proposed to avoid this limitation. They involve inserting an equivalent non-telecentric imaging system in the reference arm [22] or illuminating the sample with a converging spherical wave whose focus is conjugated with the front focus of the tube lens for full compensation [23].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive tool for a phase compensation reconstruction method in digital holographic microscopy operating in non-telecentric regime

Bogue-Jimenez,

Trujillo,

Doblas

2023

PLoS ONE

Self Cite

View full text Add to dashboard Cite

Quantitative phase imaging (QPI) via Digital Holographic microscopy (DHM) has been widely applied in material and biological applications. The performance of DHM technologies relies heavily on computational reconstruction methods to provide accurate phase measurements. Among the optical configuration of the imaging system in DHM, imaging systems operating in a non-telecentric regime are the most common ones. Nonetheless, the spherical wavefront introduced by the non-telecentric DHM system must be compensated to provide undistorted phase measurements. The proposed reconstruction approach is based on previous work from Kemper’s group. Here, we have reformulated the problem, reducing the number of required parameters needed for reconstructing phase images to the sensor pixel size and source wavelength. The developed computational algorithm can be divided into six main steps. In the first step, the selection of the +1-diffraction order in the hologram spectrum. The interference angle is obtained from the selected +1 order. Secondly, the curvature of the spherical wavefront distorting the sample’s phase map is estimated by analyzing the size of the selected +1 order in the hologram’s spectrum. The third and fourth steps are the spatial filtering of the +1 order and the compensation of the interference angle. The next step involves the estimation of the center of the spherical wavefront. An optional final optimization step has been included to fine-tune the estimated parameters and provide fully compensated phase images. Because the proper implementation of a framework is critical to achieve successful results, we have explicitly described the steps, including functions and toolboxes, required for reconstructing phase images without distortions. As a result, we have provided open-access codes and a user interface tool with minimum user input to reconstruct holograms recorded in a non-telecentric DHM system.

show abstract

Section: Introductionmentioning

confidence: 99%

Comprehensive tool for a phase compensation reconstruction method in digital holographic microscopy operating in non-telecentric regime

Bogue-Jimenez,

Trujillo,

Doblas

2023

PLoS ONE

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, realizing this condition in an off-axis geometry is difficult due to the presence of high-density fringes over the entire FoV of the camera. Dobas et al [17] proposed the use of an electrically controlled liquid lens to produce a spherical wavefront, depending on the microscope objective used, to cancel out the phase perturbation at the image plane. Ferraro et al [18] described a numerical posteriori procedure -a double exposure method in which the phase map reconstructed from a reference calibration hologram (without the object) is subtracted from the phase map reconstructed from the hologram containing the object.…”

Section: Introductionmentioning

confidence: 99%

Sampling moiré method: a tool for sensing quadratic phase distortion and its correction for accurate quantitative phase microscopy

Jayakumar¹,

Ahmad²,

Mehta³

et al. 2020

Opt. Express

View full text Add to dashboard Cite

The advantages of quantitative phase microscopy (QPM) such as label-free imaging with high spatial sensitivity, live cell compatibility and high-speed imaging makes it viable for various biological applications. The measurement accuracy of QPM strongly relies on the shape of the recorded interferograms, whether straight or curved fringes are recorded during the data acquisition. Moreover, for a single shot phase recovery high fringe density is required. The wavefront curvature for the high-density fringes over the entire field of view is difficult to be discerned with the naked eye. As a consequence, there is a quadratic phase aberration in the recovered phase images due to curvature mismatch. In the present work, we have implemented sampling moiré method for real-time sensing of the wavefront curvature mismatch between the object and the reference wavefronts and further for its correction. By zooming out the interferogram, moiré fringes are generated which helps to easily identify the curvature of the fringes. The wavefront curvature mismatch correction accuracy of the method is tested with the help of low temporal coherent light source such as a white light (temporal coherence ∼ 1.6 µm). The proposed scheme is successfully demonstrated to remove the quadratic phase aberration caused due to wavefront mismatch from an USAF resolution target and the biological tissue samples. The phase recovery accuracy of the current scheme is further compared with and found to better than the standard method called principle component analysis. The proposed method enables recording of the corrected wavefront interferogram without needing any additional optical components or modification and also does not need any post-processing correction algorithms. The proposed method of curvature compensation paves the path for a high-throughput and accurate quantitative phase imaging.

show abstract

“…9 Similarly, by inserting an electrically tunable/adjustable lens in the illumination path or in the reference arm, the parabolic phase factor or total phase distortions can be canceled out. [10][11][12] Besides, a common-path interferometer with one singlecube beam splitter is also demonstrated to physically compensate phase aberrations. 13 As for the latter scheme of multiple recordings, although the double exposure method is capable of eliminating all of the aberrations, a second object-free reference hologram has to be captured.…”

Section: Introductionmentioning

confidence: 99%

Automatic compensation of phase aberrations in digital holographic microscopy based on sparse optimization

2019

View full text Add to dashboard Cite

show abstract

Physical compensation of phase curvature in digital holographic microscopy by use of programmable liquid lens

Cited by 30 publications

References 31 publications

Comprehensive tool for a phase compensation reconstruction method in digital holographic microscopy operating in non-telecentric regime

Comprehensive tool for a phase compensation reconstruction method in digital holographic microscopy operating in non-telecentric regime

Sampling moiré method: a tool for sensing quadratic phase distortion and its correction for accurate quantitative phase microscopy

Automatic compensation of phase aberrations in digital holographic microscopy based on sparse optimization

Contact Info

Product

Resources

About